Browsing by Author "Akşit, Hasan Z."

Filter results by typing the first few letters
Now showing 1 - 2 of 2
  • No Thumbnail Available
    Item
    Apoptosis rate in malignant mammary tumours in bitches
    (Ecole Nationale Veterinaire Toulouse, 2011-03) Seyrek, Kamil; Köse, Hayrullah; Akşit, Hasan Z.; Bıldık, Ayşegül; Sandıkçı, Mustafa; İntaş, Kamil Seyrek; Özalp, Gözde Rabia; Mecitoğlu, Gülnaz Yılmazbaş; Özyiǧit, Musa Özgür; Uludağ Üniversitesi/Veterinerlik Fakültesi/Doğum, Jinekoloji ve Üreme Anabilim Dalı.; Uludağ Üniversitesi/Veterinerlik Fakültesi/Patoloji Anabilim Dalı.; 0000-0003-2817-3221; AAH-7292-2019; AAG-4440-2019; AAR-6478-2021; 6603409870; 23985710500; 36457964000; 6507338060
    As the equilibrium between cell division and cell death (apoptosis) determines the growth of a tumour, the aim of the study was to evaluate the apoptosis frequency in dog mammary tumours both by the TdT-mediated dUTP-biotin nick end Libelling (TUNEL) method and by the ELISA detection of nucleosoms in cell homogenates. For that, apoptosis rates were investigated in 35 malignant tumours (16 malignant mixed tumours, 12 lobular adenocarcinomas, 3 cirrhotic adenocarcinomas and 4 myoepitheliomas) and in 10 normal mammary tissues. Whereas the apoptotic cells were found abundant and homogeneously distributed throughout the healthy breast tissues by the TUNEL method, they appeared less numerous in malignant tumours and were essentially located within mammary acini in few areas. The average amounts of nucleosoms proportional to the absorbance at 405 nm in the ELISA test in cell homogenates from malignant tumours were lower than in the healthy mammary tissues but according to the tumour type, the difference with controls was significant only for the malignant mixed tumours (P < 0.05). These results show that apoptosis rate tended globally to decrease in dog malignant mammary tumours, favouring in this way the selection and expansion of apoptosis-resistant neoplastic cells.
  • No Thumbnail Available
    Item
    Protective effect of 2-aminoethyl diphenylborinate on acute ischemia-reperfusion injury in the rat kidney
    (Elsevier, 2014-04) Yıldar, Murat; Akşit, Hasan Z.; Korkut, Oǧuzhan; Sunay, Bahar; Seyrek, Kamil; Özyiǧit, Musa Özgür; Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; AAR-6478-2021; 6507338060
    Background: To investigate the protective effect of 2-aminoethyl diphenylborinate (2-APB) against ischemia-reperfusion (I/R) injury in the rat kidney by an experimental study. Materials and methods: Thirty male Sprague-Dawley rats were randomly divided into the following three groups: (1) sham group, (2) I/R group, and (3) I/R + 2-APB group. Renal I/ R injury was induced by clamping the left renal pedicle for 45 min after right nephrectomy, followed by 3 h of reperfusion. The therapeutic agent 2-APB was administered intravenously at a dose of 2 mg/kg 10 min before renal ischemia. Glutathione, superoxide dismutase, total antioxidant capacity, malondialdehyde, tumor necrosis factor a, interleukin 6, aspartate aminotransferase, alanine aminotransferase, and creatinine levels were measured from blood samples, and the rats were sacrificed subsequently. Tissue samples were scored histopathologically. Visualization of apoptotic cells was performed using the terminal deoxynucleotidyl transferase dUTP nick end labeling staining method. Results: 2-APB significantly reduced serum malondialdehyde, tumor necrosis factor a, interleukin 6, aspartate aminotransferase, alanine aminotransferase, and creatinine levels in the I/R injury group. However, glutathione, superoxide dismutase, and total antioxidant capacity levels increased significantly. Histopathologic scores were significantly better and the rate of apoptosis was lower in the 2-APB group. Conclusions: 2-APB reduces oxidative stress and damage caused by renal I/R injury. The results of this study demonstrate that 2-APB can be used as an effective agent against I/R injury in the kidney.