Browsing by Author "Demirbilek, Serpil Kahya"

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A surveillance for avian coronavirus infectious bronchitis virus, infectious laryngotracheitis virus, avian metapneumovirus, and avian reovirus in poultry fllocks with respiratory signs in Turkiye
(Tubitak Scientific & Technological Research Council Turkey, 2022-01-01) Çöven, Fethiye; Ardıçlı, Özge; ARDIÇLI, ÖZGE; Demirbilek, Serpil Kahya; KAHYA DEMİRBİLEK, SERPİL; Çarlı, Kamil Tayfun; CARLI, KAMİL TAYFUN; Bursa Uludağ Üniversitesi/Veteriner Fakültesi.; 0000-0001-6077-0478; 0000-0003-1928-7630; AAG-7421-2021
In this study, avian coronavirus infectious bronchitis virus (IBV), infectious laryngotracheitis virus (ILTV), avian metapneumovirus (AMPV), and avian reovirus (ARV) were evaluated in broiler and layer flocks. For this purpose, tracheal swabs from 48 broiler and 45 layer flocks with respiratory signs were inoculated SPF embryonated chicken eggs for virus isolation. The viruses were identified by real-time PCR. Results showed that the most common virus in both broiler and layer farms was IBV with incidence rates of 58.33% and 46.67%, respectively. ILTV, AMPV, and ARV incidences in the samples were found to be 22.22%, 13.33%, and 4.44% in layer flocks while 2.08%, 8.33%, and 20.83% in broilers, respectively. The numbers of IBV+AMPV and IBV+ARV coinfections were 5 (11.11%) and 1 (2.22%) in layers, whereas, 1 (2.08%) and 5 (10.42%) broilers, respectively. In addition, 2 broiler flocks (4.17%) had triple infection with IBV, AMPV, and ARV. ILTV was detected always alone from the samples of layer and broiler flocks. Sequencing of S1 gene of selected IBV TR/L45 and TR/B42 isolates showed similarities with IS/1494/06 (HM131453) at the rates of 98.98% and 99.69%, respectively, while TR/L37, TR/L38, and TR/L39 isolates were identical to 4/91 (KF377577) vaccine strain at the rates of 99.01%, 99.01%, and 98.76%, respectively. Sequencing analysis of the ICP4 and TK genes of ILTV isolates revealed that they were all field strains with low virulence. The present data represent actual information on the genotypes of IBV and ILTV circulating in poultry flocks in Turkiye.
Comparison of cytological, microbiological and histopathological findings of genital tracts in cows with different degree perineal conformation disorder
(Sivar-soc Italiana Veterinari Animali Reddito, 2021-02-01) Intas, Kamil Seyrek; Salci, Emsal Sinem Ozdemir; ÖZDEMİR SALCI, EMSAL SİNEM; Yavas, Ozkan; YAVAŞ, ÖZKAN; Ardicli, Ozge Yilmaz; Sonmez, Gursel; SÖNMEZ, GÜRSEL; Demirbilek, Serpil Kahya; KAHYA DEMİRBİLEK, SERPİL; Ardicli, Sena; ARDIÇLI, SENA; Bursa Uludağ Üniversitesi/Veteriner Fakültesi.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı.; 0000-0001-6077-0478; 0000-0003-2758-5945; KHD-4075-2024; O-3394-2019; AAG-7421-2021; AAH-5294-2021
The aim of this study is to investigate cytological, microbiological and histopathological findings of the genital tracts in cows with different degree perineal conformation (PC) disorder. Totally, 28 cows brought the slaughterhouse were materials of the study. Information about to age, body weight, reproductive status, number of parturitions, days in milk (DIM) and the reason for slaughtering of the cows was obtained in the anamnesis learned from the owners. Before slaughtering, cows were evaluated in terms of PC and then they were divided into four groups with equal numbers of cows according to PC disorders: group I (GRI) normal; group II (GRII) mild; group III (GRIII) moderate and group IV (GRIV) severe. PC disorders of the cows were graded on a scale in terms of vulvar angle, vulvar length over ischial arch, depth of anus and perineal length. A body condition score (BCS) was also evaluated in the cows. The perineal region and vagina were inspected for vaginal discharge and vaginal mucosal appearance, respectively. Pneumovagina was classified as negative, suspicious and positive. Sterile swab samples were taken from vagina, cervix and uterus for bacteriological culture and identification. For cytological examination, smears were taken from vagina, cervix and uterus. Tissue samples were taken from vagina, cervix and uterus for histopathological examination. Statistically, Pearson's correlation, Fisher's exact tests and regression analysis were performed for all data. Clinically, characters of the vaginal discharge (serous, foamy, mucous, purulent and urine-mixed) and vaginal hyperemia increased in GRIII and GRIV. Microbiologically, as parallel to the PC disorder, E. coli in Enterobacteriaceae family was the most common bacterium in Group III and Group IV. As PC disorder in the groups increased, cytological examination findings were found to be significant, similar to clinical and microbiological examination. However, histopathological examination gave more meaningful results in groups. Statistic results pointed out that difference between the groups in terms of vaginal mucosa color, cervical and uterine microbiology was significant. In conclusion, PC disorder in cows constitutes predisposition for many genital canal diseases that may be the cause of infertility. The presence of genital canal diseases can be suspected in cattle using the PC scale. Therefore, it is appropriate to evaluate the health status of the genital canal with other diagnostic methods (microbiology, cytology and histopathology), especially in cows with PC disorder.
Comparison of mycoplasma gallisepticum infection in different samples and ages of chicken breeder flocks
(Facta-fundacio Arnco Ciencia Tecnologia Avicolas, 2020-01-01) Demirbilek, Serpil Kahya; KAHYA DEMİRBİLEK, SERPİL; Ardıçlı, Özge; ARDIÇLI, ÖZGE; Çarlı, Kamil Tayfun; CARLI, KAMİL TAYFUN; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.; 0000-0001-6077-0478; 0000-0001-6045-8644; AAG-7421-2021; AAH-2842-2021
This study aimed to compare method-based and newly developed sample-based methods for Mycoplasma gallisepticum (MG) detection in different samples of breeder flocks suffering from respiratory disease problems by using culture, real-time PCR (rPCR) and ELISA from chicks and embryonated eggs. Overall, 450 samples of 19-day-old chicken embryo's trachea, 450 samples of 8-day-old chicken tracheal swabs and 900 blood samples of 20-, 27-, 34-, 40- and 46-week-old breeder chickens from 5 flocks were sampled for 26 weeks, and were all tested for MG by culture, MG-rPCR and MG-ELISA. Culturing assays and rPCR were applied to 450 mixture samples from 19-day-old chicken embryo's trachea and 450 tracheal swab samples (each pooled into groups of 3) from 8-day-old chicks from the same flocks. Also, 900 blood samples from the same 5 breeder flocks suffering from respiratory disease problems were tested by MG-ELISA.In individual sample-based analyses, 55 (18.3%) of the 300 pooled swab samples were positive for MG using culture methods, and 106 (35.3%) of the same samples were found positive by rPCR (sensitivity, specificity). The ELISAs indicated that 252 (28%) of the 900 breeding blood samples were MG seropositive. Using age-based analyses, the most positive period was 46 weeks, followed by 40 weeks, 34 weeks, 27 weeks and at least 20 weeks, in order of decreasing seropositivity. When comparing the culture and rPCR results of the two different sampling methods, chicken embryo's trachea yielded more positive results than did tracheal swabs from the same flocks. In conclusion, rPCR is a highly specific, sensitive and reliable method for MG identification.
Comparison of the efficacy of enrofloxacin and lactobacillus plantarum cell-free supernatant treatments on vaginitis in ewes
(Bursa Uludağ Üniversitesi, 2022-08-10) Güner, Barış; Kısadere, İhsan; Tavşanlı, Hakan; Demirbilek, Serpil Kahya; Keskin, Abdulkadir; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi.; 0000-0001-6138-7163; 0000-0001-7149-0009
The aim of the study was to investigate the effect of different intravaginal treatment strategies on the vaginal discharge score, vaginal microbiota, bacterial and Enterobacteriaceae counts in nulliparous Merino ewes. All ewes (n=45) received intravaginal sponges containing 60 mg medroxyprogesterone acetate for 7 days and allocated into three equal groups (n=15). Sponges were injected Lactobacillus plantarum cell-free supernatant (SUPER), enrofloxacin (ENRO), or physiologic saline (CON) prior to sponge insertion. At sponge removal, 500 IU equine chorionic gonadotropin (eCG) were administered in all ewes. For the detection of vaginal microbiota, bacterial and Enterobacteriaceae counts, samples were collected prior to sponge insertion, at sponge withdrawal, and 48 h later after sponge withdrawal. Vaginal discharge score was not different in ENRO (2.26±0.18) and SUPER (2.20±0.14) compared to CON (2.46±0.16). The time-dependent alteration was significant for the mean bacterial and Enterobacteriaceae count in all groups (P<0.05). Bacterial counts were found to be lower in ENRO (5.50±0.17) than SUPER (6.31±0.19) and CON (6.07±0.15) at sponge removal (P<0.05). In addition, SUPER (3.74±0.21) and ENRO (3.49±0.27) had lower Enterobacteriaceae counts compared to CON (4.78±0.21) at sponge removal (P<0.01). The most frequently isolated bacteria species were Trueperella pyogenes (28.9%) and Escherichia coli (46.7%). In conclusion, treatment with enrofloxacin or cell-free supernatant decreased the Enterobacteriaceae counts in ewes. Comprehensive studies are needed to assess the effectiveness of lactic acid bacteria as an antibiotic-free treatment strategy on vaginitis in ewes that were synchronized with progesterone impregnated intravaginal sponge.
Comparison of the endocrinological and immunological results of different induction of parturition methods in ewes
(Georg Thieme Verlag, 2018-02) Salcı, Emsal Sinem Özdemir; Demirbilek, Serpil Kahya; Güneş, Nazmiye; Goncagül, Gülşen; Uzabacı, Ender; Carlı, Tayfun; Seyrek, Kamil İntaş; Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Biyokimya Anabilim Dalı.; Uludağ Üniversitesi/Meslek Yüksekokulu/Mennan Pasinli.; Uludağ Üniversitesi/Veteriner Fakültesi/Biyoistatistik Anabilim Dalı.; 0000-0002-9634-0055; AAH-2842-2021; AAK-6555-2021; AAH-7292-2019; AAH-5294-2021; AAI-1930-2021; 55921595900; 35368679200; 6506008074; 8350555500; 55347697800; 7005947635; 6603409870
Objective: This study aimed to compare different induction of parturition (IP) methods in terms of endocrinological and immunological parameters in ewes and lambs. Materials and methods: A total of 24 ewes and their respective 24 lambs were studied. Groups of six ewes were induced on the 138th day: groups I (control), II (dexamethasone), III (aglepristone) and IV (aglepristone + dexamethasone). Blood samples were taken from the ewes from IP to 2 days postpartum at 12-hour intervals for the prolactin and oxytocin analyses. Colostrum and blood samples were collected from the lambs at 0, 12, 24, 36 and 48 hours for the IgG analysis. The prolactin, oxytocin and IgG levels were measured by ELISA. Results: A significant difference was found in prolactin levels in all groups and in the colostral IgG level in group IV with respect to the sampling time. Additionally, a significant difference in prolactin level was found at 24 hours postpartum between group IV and groups I and III. A significant difference in the colostral IgG level was determined at 24 hours after parturition between group III and the other groups and at 48 hours after parturition between groups II and III. A positive and significant correlation between the colostral and serum IgG levels of the lambs was found at 24 hours in groups I and II. Conclusion and clinical relevance: Although varying individual results were found within the groups, the endocrinological and immunological results did not suggest any superiority among the IP methods. Considering the presented study results, a single dose of aglepristone may be used alone or in combination with dexamethasone for IP.
How do we use molecular knowledge in diagnosis and control of pandemic avian viruses?
(Aves, 2022-01-01) Ardıçlı, Özge; Demirbilek, Serpil Kahya; Coven, Fethiye; Çarlı, Kamil Tayfun; ARDIÇLI, ÖZGE; KAHYA DEMİRBİLEK, SERPİL; CARLI, KAMİL TAYFUN; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.; 0000-0001-6077-0478; 0000-0003-1928-7630; AAG-7421-2021; E-3867-2010; CNE-1191-2022
Pandemic respiratory viruses of poultry have caused significant economic losses in the poultry industry since the 1930s, and molecular and genetic techniques are widely used for diagnosis and control of the infections. Knowledge of changes in the genetic and antigenic characteristics of the pandemic viruses during the time can be really important for human pandemic viruses such as severe acute respiratory syndrome virus-coronavirus-2 and human influenza virus. The use of these techniques plays a vital role in preventing the faulty results and the possible financial losses that may occur due to the limited findings obtained from conventional laboratory tests. In the light of this information, the purpose of this review is to provide an up-to-date assessment of the diagnosis and prevention of major respiratory viruses in poultry and a general and field-oriented scientific perspective that may be useful in the industry. In this context, current approaches for diagnosis and vaccination applications developed using molecular methods based on avian coronavirus infectious bronchitis virus, avian paramyxovirus-1 virus, and avian influenza virus, which are pandemic, are discussed, and solution suggestions for an effective fight are presented.
Identification and antimicrobial susceptibility of microbial agents of otitis externa in dogs
(Polish Soc Veterinary Sciences Editorial Office, 2019-02-01) Demirbilek, Serpil Kahya; KAHYA DEMİRBİLEK, SERPİL; Yılmaz, Özge; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/ Mikrobiyoloji Anabilim Dalı.; 0000-0001-6077-0478; AAH-2842-2021; AAG-7421-2021
In this study, a total of 277 unmedicated dogs with otitis externa were used. Overall, 413 agents were isolated from 277 ear swab samples; 52.7% of the cases were mono-infections (146 cases), and 37.1% of the cases were poly-infections (103 cases). In 10.1% (28) of the cases, neither bacteria nor yeasts were isolated. Coagulase-positive Staphylococcus spp. were the most frequently isolated bacteria and were found in 90 (21.8%) of the samples. Fifty-eight samples, (14%) were positive for Staphylococcus aureus, 51 (12.3%) for Pseudomonas aeruginosa, 27 (6.5%) for Proteus mirabilis, 27 (6.5%) for Malassezia pachydermatis, 21 (5%) for Corynebacterium spp., 21 (5%) for beta-haemolytic Streptococcus spp., 15 (3.6%) for Staphylococcus pseudointermedius, 12 (2.9%) for Proteus spp., 12 (2.9%) for Escherichia coli, 9 (2.1%) for Acinetobacter calcoaceticus, 7 (1.6%) for Trichophyton mentagrophytes, 5 (1.2%) for Staphylococcus auricularis, and 46 (11.1%) for different bacteria and yeasts. A total of 14 different bacteria and yeasts were isolated and identified. Kirby-Bauer antibiotic susceptibility testing was carried out for 10 different antibiotics. The bacterial isolates were found to be resistant to amoxicillin-clavulanic acid (45%), gentamycin (28%), ampicillin/cloxacillin (69%), tobramycin (28%), amikacin (23%), enrofloxacin (47%), chloramphenicol (58%), doxycycline (65%), lincomycin/spectinomycin (58%) and polymyxin B (62%). In conclusion, it is important to test the antimicrobial sensitivity of aetiological agents of otitis externa before treatment so as to prevent the development of antibiotic resistance in bacteria and yeasts.
Isolation and biotyping of Brucella melitensis from aborted sheep and goat fetuses
(TÜBİTAK, 2009) Büyükcangaz, Esra; Şen, Ayşin; Demirbilek, Serpil Kahya; Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.; 0000-0003-4337-577X; 0000-0002-9212-8743; AAL-2323-2020; AAH-1820-2021; AAH-2842-2021; 25649139400; 7401592869; 35368679200
The possible role of Brucella spp. in 65 abortion cases, 55 from sheep and 10 from goats, occurring in the birth seasons of 2004 and 2005 in northwestern Turkey was investigated. Colony morphology, agglutination by acriflavin, H2S production, CO2 requirement, dye sensitivity in thionin, basic fuchsin, growth characteristics in streptomycin, lysis with Tbilisi phage, and agglutination with monospecific A- and M antisera were examined for identification and biotyping. The isolates from 21 of 55 sheep and 1 of 10 goat abortion cases were identified as Brucella melitensis. Biotyping revealed that 14, 6, and 1 of 22 Brucella spp. were B. melitensis biotype 3, B. melitensis biotype 1, and B. melitensis biotype 2, respectively. One strain isolated from a sheep abortion case was H2S- producing and was identified as atypical B. melitensis.
Kedi ve köpeklerde Salmonella taşıyıcılık oranı ve antibiyotik duyarlılıklarının belirlenmesi
(Bursa Uludağ Üniversitesi, 2024-05-30) Yıldız, Merve; Demirbilek, Serpil Kahya; Bursa Uludağ Üniversitesi::Enstitüler::Sağlık Bilimleri Enstitüsü / Veterinerlik Mikrobiyolojisi Anabilim Dalı; 0000-0003-2883-2338
Salmonelloz gıda kaynaklı zoonotik bir hastalık olarak kabul edilmesine rağmen, evcil hayvanlar sahipleriyle yakın temas halinde olmaları nedeniyle antimikrobiyal dirençli Salmonella bakterilerinin insanlara yayılmasında önemli bir rol oynayabilirler. Bu çalışmanın amacı, Türkiye'deki evcil kedi ve köpeklerde Salmonella prevalansını, risk faktörlerini, bazı virülans faktörlerini, serotiplerini ve antimikrobiyal direnç profillerini belirlemek ve halk sağlığı riskini değerlendirmek ve ayrıca, Salmonella-pozitif ve Salmonella-negatif hayvanların bağırsaklarındaki laktik asit bakterilerinin(LAB) makroskopik karşılaştırmasını yapmaktır. Üç yüz kırk sekiz rektal sürüntüörneğinde Salmonella'nın tanımlanmasında; Uluslararası Standartlar Örgütü (ISO) 6579-1:2017 ve Gıda ve İlaç İdaresi (FDA) yöntemleri olmak üzere 2 kültür yöntemi etkinliği karşılaştırılmıştır. Elde edilen Salmonella’ların identifikasyonu BD Phoenixotomatize sistemi ve 16S rDNA gen analizi ile gerçekleştirilmiştir. Pozitif izolatlarWhite-Kauffmann-Le Minor şemasına göre lam aglütinasyon yöntemi kullanılarak serotiplendirilmiş ve bazı virülans genlerinin (invA ve stn) varlığı polimeraz zincir reaksiyonu (PCR) ile değerlendirilmiştir. Antimikrobiyal aktivite, Kirby-Bauer diskdifüzyon yöntemiyle, Clinical and Laboratory Standards Institute (CLSI) kılavuzlarına göre test edilmiştir. Salmonella prevalansı köpeklerde %5,73 (9/157) ve kedilerde %0,0 (0/191) olarak bulunmuştur. Sekiz (8/9) izolat ISO yöntemiyle ve 5 (5/9) izolat FDA yöntemiyle kültüre edilebilmiştir. Makroskopik sonuçlar Salmonella etkenlerinin bağırsaklardaki LAB üzerinde herhangi bir etkisi olmadığını ortaya koymuştur. Üç farklı Salmonella serotipi tespit edilmiş ve tüm izolatlar virülans genleri açısındanpozitif bulunmuştur. Antibiyotik direnç profili, izolatların %11,1'inin MDR (Multidrug resistance) olduğunu ve en yüksek direncin siprofloksasin için bulunduğunu göstermiştir. Köpek izolatlarında MDR dirençli S. Virchow ve karbapenem dirençli S. Enteritidis tespit edilmiştir. Çiğ et tüketimi ile Salmonellataşıyıcılığı arasında ilişki anlamlı bulunmuştur (p<0.01). Çalışma sonucunda, köpeklerin Salmonella enfeksiyonunun potansiyel taşıyıcıları olabileceği sonucuna varılmıştır. Salmonella' nın ishalli köpekler yerine sağlıklı köpeklerden izole edilmesi, asemptomatik taşıyıcılığa dikkat çekmiştir. Zoonotik Salmonella izolatları arasında direncin ortaya çıkması halk sağlığı için de salmonellozun önemli bir tehdit olduğunu ortaya çıkarmıştır.
Pathogens isolated from bovine clinical mastitis and their antimicrobial resistance
(Polish Soc Veterinary Sciences Editorial Office, 2022-01-01) Ardıçlı, Özge; Demirbilek, Serpil Kahya; Carlı, Kamil Tayfun; ARDIÇLI, ÖZGE; KAHYA DEMİRBİLEK, SERPİL; CARLI, KAMİL TAYFUN; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı; 0000-0001-6077-0478; AAG-7421-2021; E-3867-2010; CNE-1191-2022
This study aimed to isolate aerobic and microaerophilic bacteria from mastitis milk samples, as well as to determine their antibiotic resistance. A total of 196 bovine mastitis milk samples were tested by standard bacteriological methods and with API identification test kits. Antimicrobial susceptibility testing was performed by the Kirby-Bauer disk diffusion method. The results revealed that the predominant isolate was S. aureus, with an isolation rate of 28%, followed by Streptococcus spp. (27%) and E. coli (19%). Isolation rates for Corynebacterium spp., Mycoplasma spp., and Pseudomonas aeruginosa were 11%, 6%, and 4%, respectively. Compared to the bacteria mentioned above, lower percentages were observed for Trueperella pyogenes (2%), Pasteurella multocida (2%), and Klebsiella pneumoniae (1%). A broad evaluation of antimicrobial resistance showed that the pathogens were resistant to tetracycline (68.63%), oxytetracycline (41.57%), ampicillin (39.08%), ceftiofur (38.1%), cephalexin (32.26%), penicillin (31.25%), amoxicillin/clavulanic acid (24.53%), enrofloxacin (24.44%), gentamycin (23.68%), and trimethoprim/sulfamethoxazole (22.09%). This study demonstrated that the sources of bacteria isolated from mastitis bovine milk samples were both contagious and environmental. More importantly, the present results demonstrate a critically high antimicrobial resistance in dairy cattle. For instance, E. coli isolates showed a crucial resistance to commonly used and recommended antimicrobials, including ceftiofur (100%), cephalexin (83.33%), and tetracycline (94.44%). The results of this study may provide valuable information about clinical aspects of bovine mastitis infections and current antimicrobial resistance levels in dairy cattle.
Serum C-reactive protein, erythrocyte sedimentation rate, and white blood cell count in romanov sheep with infectious keratoconjunctivitis
(Hellenic Veterinary, 2018) Akgül, Gülşah; Akgül, Mustafa Barış; Irak, Kıvanç; Çelik, Özgür Yaşar; Demirbilek, Serpil Kahya; Demirbilek, Serpil Kahya; Uzabacı, Ender; Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Bioistat Bölümü.; AAH-2842-2021
This study aimed to evaluate the use of erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) and white blood cell count (WBC) as markers of the severity of inflammation in the eyes of the Romanov breed sheep with infectious keratoconjunctivitis. A total of 10 Romanov breed sheep between the 1.5 and 2 years of age, including the ones carrying infectious keratoconjunctivitis (G1, n = 5) and healthy ones (G2, n = 5), which were housed under the same care and nutrition conditions were examined, in a sheep breeding enterprise within the boundaries of Siirt province. Staphylococcus aureus sp., Clostridium spp., and Penicillium spp. were detected based on the microscopic morphology of the colonies in swabs collected from the eyes of sick animals. Biochemical tests were performed relevant to the suspected agents while no growth was detected in the swabs of the control group. There was a statistically significant difference in serum CRP and WBC levels between the G1 and G2 groups (p<0.05). No statistically significant difference was found between the values of the other parameters tested. Higher levels of CRP and WBC were determined in sheep having infectious keratoconjunctivitis, are compared to those in healthy animals.