Browsing by Author "Failing, Klaus H."

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    Effect of experimental endotoxemia on thrombelastography parameters, secondary and tertiary hemostasis in dogs
    (Wiley, 2011) Failing, Klaus H.; Moritz, Andreas; Bauer, Natali B.; Eralp, Oya; Yılmaz, Zeki; Uludağ Üniversitesi/Veterinerlik Fakültesi/Dahiliye Anabilim Dalı.; 0000-0002-4242-8609; 0000-0001-9836-0749; AAG-2943-2020; 24472964600; 35944810500
    Background Thrombelastography (TEG) and indicators of secondary and tertiary hemostasis might be altered in dogs with endotoxemia. Hypothesis Endotoxemia influences measures of coagulation in dogs. Animals Ten healthy cross-bred dogs. Material and Methods Prospective laboratory study between controls (n = 5) receiving 0.9% saline IV and the study group (n = 5) treated with low-dose lipopolysaccharide (0.02 mg/kg IV). Physical examination and sampling for measurement of leukocytes, platelets, and coagulation variables were performed at time points 0, 1, 4, and 24 hours. Coagulation variables included kaolin-activated TEG, 1-stage prothrombin time (OSPT), activated partial thromboplastin time (aPTT), fibrinogen, factor VIII, antithrombin, protein C, protein S, activated protein C (APC)-ratio calculated from aPTT with and without presence of APC), and D-Dimers. Results Endotoxemia-induced clinical signs included lethargy (n = 5/5), diarrhea (n = 4/5), emesis (n = 4/5), and abdominal pain (2/5). After 1 hour there was severe leukopenia (2.5 +/- 0.7 x 109/L; mean +/- SD, P < .0001) and a 2.2-fold increase in D-Dimers (0.81 +/- 0.64 mg/L, P < .0001). After 4 hours there was hyperthermia (40.3 +/- 0.4 degrees C, P < .0001) and increases in OSPT (10.5 +/- 2.7 seconds, P < .0001), aPTT (16.7 +/- 5.2 seconds, P = 0.002). A significant decrease in fibrinogen (1.5 +/- 1.0 g/L, P = 0.001), protein C (31 +/- 33%, P <.0001), protein S (63 +/- 47%, P < .0001), TEG alpha (58 +/- 19, P = .007), and TEG maximal amplitude (50 +/- 19 mm, P = .003) was seen compared with the controls. APC-ratio rose significantly (2.5 +/- 0.2, P < .0001) without exceeding the reference interval (n = 4/5). Conclusion and Clinical Importance D-Dimers are the earliest indicator for endotoxemia-associated coagulation abnormalities followed by decreased protein C concentration. APC-ratio and TEG were not good screening variables.
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    Relation between intravascular electrolyte level and course of parturition in dairy cows
    (Georg Thieme, 2013) Failing, Klaus H.; Bostedt, H.; İntaş, Kamil Seyrek; İntaş, Deniz Seyrek; Mecitoğlu, Gülnaz Yılmazbaş; Uludağ Üniversitesi/Veterinerlik Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; Uludağ Üniversitesi/Veterinerlik Fakültesi/Cerrahi Anabilim Dalı.; 0000-0003-2817-3221; AAG-4440-2019; AAH-7292-2019; 6603409870; 6506990178; 36457964000
    Objective: To determine the intravascular electrolyte status in dairy cows with respect to age and different courses of parturition to clarify etiological factors influencing peri- or intrapartal imbalances of electrolyte homeostasis. Material and methods: A total of 64 cows at birth were evaluated (primiparous n = 34, pluriparous n = 30). Thirty-three cows showed normal delivery, while 31 cows had a complicated birth. Blood samples were collected intra partum (i. p.) and 2, 4, 6, 8, 12, 16,24 h post partum (p. p.) as well as [2, 3, 4, 5, 7, 10 days p. p. and levels of total calcium (Ca-total), ionized calcium (Ca-ion), inorganic phosphate (P-a), Mg, Na, K, Cl were determined. Results: The results revealed that electrolytes show great fluctuation during and immediately p. p. in relation to age. Already during parturition pluriparous, cows had a lower Catotal and P-a concentration compared to primiparous animals. Cows with dystocia exhibited a more intensive and longer lasting decrease of Ca compared to cows with normal birth. In relation to age and severity of birth P-a concentration showed a differing but basically typical course for this electrolyte. Mg, Na, K and Cl concentrations were higher during and immediately after birth compared to p. p. values. Until day 10 p. p. these electrolyte concentrations declined more in older cows with dystocia compared to younger animals. However, the influence of dystocia on concentration of these electrolytes was milder in contrast to Ca and P-a. Conclusions: In summary, primarily older cows are predisposed to imbalances of electrolyte, homeostasis intra partum and at the beginning of the lactation. These, changes are potentiated in case of complications during parturition. Intravascular Ca-total, Ca-ion as well as P-a are most severely affected. Clinical relevance: These results may constitute the basis for a comprehensive metaphylaxis during the peripartal period, especially in cows after dystocia, to positively influence the early convalescence phase.