Browsing by Author "ARDIÇLI, SENA"

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Analysis of bovine beta-casein a1 and a2 allele frequency in holstein-friesian cows by real-time pcr with fluorescent hybridization probes
(Univ Zagreb Vet Faculty, 2023-01-01) Ardıçlı, Sena; ARDIÇLI, SENA; Balcı, Faruk; BALCI, ÖMER FARUK; Şamlı, Hale; ŞAMLI, HALE; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı.; 0000-0003-2758-5945; 0000-0003-2382-1330; O-3394-2019
A2 milk popularity is increasing across the world and novel molecular techniques have been evaluated to develop reliable methods. This study aimed to genotype Holstein-Friesian cows concerning their A1/A2 status using Real-time PCR assay with the specifically designed FRET hybridization probes. In this context, DNA samples were obtained from 310 Holstein-Friesian milk samples. Concerning the Real-time PCR assay, the melting temperature of each amplicon was analyzed and the melting data was converted to a derivative plot using the LightCycler 480 System. The sensor probe was designed to match the wild-type sequence in the target DNA. In the Real-time PCR assay, the melting peaks obtained in the Real-time PCR assay were highly decisive and consistent for each genotype regarding CCT?CAT alteration. The results indicated a remarkably high frequency of the A2 allele (68%) and a considerable frequency of heterozygous animals (0.41). Population genetic analysis showed intermediate levels of genetic variability and biodiversity. The A2-herd conversion process is a complex process consisting of genetic testing of both cows and calves, evaluating replacement rates, and the conversion of heterozygotes by using A2-genotyped bull semen. In this sense, the key point is a reliable and rapid genotyping method to produce A1-free milk. This study suggests that Real-time PCR assay with the specifically designed FRET hybridization probes is a preferable method for A2 genotyping, and may be useful for further studies and instructive for companies or breeders who aim to produce A2 milk.
Analysis of FecB, BMP15 and CAMP gene mutations in sakiz sheep
(Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 2015-07-01) Dincel, Deniz; Ardıçlı, Sena; Soyudal, Bahadir; Er, Mehlika; Alpay, Fazlı; Şamlı, Hale; Balcı, Faruk; DİNÇEL, DENİZ; ARDIÇLI, SENA; Soyudal, Bahadir; Er, Mehlika; ALPAY, FAZLI; ŞAMLI, HALE; BALCI, ÖMER FARUK; Uludağ Üniversitesi/Veteriner Fakültesi; 0000-0002-8015-9032; 0000-0003-2758-5945; 0000-0003-2382-1330; O-3394-2019; AAE-4562-2019; AAH-6488-2021; AAH-6192-2021; FXS-6181-2022; CNK-1840-2022; I-5009-2019
Fertility traits, such as the ovulation rate and the number of offspring at birth, are genetically regulated by fecundity genes. This study was performed to identify DNA polymorphisms in Booroola (FecB), Galway (FecX(G)), Inverdale (FecX(I)) and Calpastatin (CAST) genes in Sakiz sheep. A total of 71 ewes were genotyped for gene or allelic polymorphisms in the genes listed above using the PCR-RFLP method. The results obtained from this study indicated that all of the Sakiz ewes sampled were non-carriers for FecB, FecX(G) or FecX(I) mutations. However, genotypic frequencies in the CAST gene were 0.59, 0.36 and 0.05 for AA, AB and BB, respectively. A significant deviation from Hardy-Weinberg equilibrium for the CAST gene was not observed in the investigated breed (P>0.05). As a result, more extensive screening is required as tests for newly discovered mutations are developed. Additionally, this study is the first to report a genetic polymorphism in the CAST gene in Sakiz sheep.
Association of the caprine calpastatin Msp polymorphism with growth and reproduction traits in Saanen goats
(Hellenic Veterinary Medical Soc, 2021-07-01) Ardıçlı, Sena; Üstüner, Hakan; Arslan, Oğuzhan; ARDIÇLI, SENA; ÜSTÜNER, HAKAN; ARSLAN, OĞUZHAN; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Zootekni Anabilim Dalı; 0000-0003-2758-5945; 0000-0002-1439-8502; O-3394-2019; GRY-9670-2022; JHO-8192-2023
This study was designed to evaluate the effects of calpastatin (CAST) Mspl polymorphism on some growth and reproduction traits, including birth weight, first breeding weight, litter size, and average daily weight gain in Saanen goats. In this sense, blood samples obtained from 73 purebred female Saanen goats were used for genotyping. Genomic DNA was isolated by the phenol-chloroform method and used to determine CAST genotypes, including MM, MN, and NN, by means of the PCR-RFLP method. The population genetic parameters were estimated based on allelic distribution and the data were statistically analysed using analysis of variance (ANOVA) using a general linear model (GLM). Results revealed that N allele frequency was remarkably high (0.64) and the MM genotype was not present. The frequency of the heterozygous genotype was 59.62%. Concerning ANOVA results, significant differences were found between genotypes of the CAST locus concerning birth weight (P<0.05). In this respect, animals with the NN genotype were associated with higher birth weight means (2.85 +/- 0.29 kg) compared to heterozygous animals (2.53 +/- 0.24 kg). There was no significant association between the CAST marker and any of the remaining phenotypic traits evaluated. The present results suggest that focusing on this genomic region may be particularly useful in improving birth weight in goats which can be considered as an early indicator of post-weaning animal growth and survival.
Beef colour defined based on ph in holstein bull carcasses
(Agricultural Research Communication Centre, 2019-11-01) ARDIÇLI, SENA; DİNÇEL, DENİZ; Balci, Faruk; BALCI, ÖMER FARUK; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı.; 0000-0003-2758-5945; 0000-0002-8015-9032; 0000-0003-2382-1330; AAH-6192-2021; O-3394-2019
The present study was carried out on 510 Holstein-Fresian bulls and it aimed to evaluate the effects of season, age at slaughter and pH on meat colour characteristics. An additional aim of this analysis was to determine the impact of environmental factors including season and slaughter age on meat pH. A cluster analysis applied to all the colour coordinates divided the samples into six groups according to their ultimate pH. L*, a*, b* and C* values were significantly higher in cattle slaughtered in the winter season. Moreover, a significant effect of age at slaughter on colour coordinates was found with the exception of a* value. Meat from bulls slaughtered at 16/17 months of age was characterized by brighter colour. Among the pH groups, the highest value of L* was found in meat with pH<5.40, whereas, the highest a* and C* in pH 5.415.50. The present results may be useful and indicative for both beef industry and future studies on meat colour.
Comparison of cytological, microbiological and histopathological findings of genital tracts in cows with different degree perineal conformation disorder
(Sivar-soc Italiana Veterinari Animali Reddito, 2021-02-01) Intas, Kamil Seyrek; Salci, Emsal Sinem Ozdemir; ÖZDEMİR SALCI, EMSAL SİNEM; Yavas, Ozkan; YAVAŞ, ÖZKAN; Ardicli, Ozge Yilmaz; Sonmez, Gursel; SÖNMEZ, GÜRSEL; Demirbilek, Serpil Kahya; KAHYA DEMİRBİLEK, SERPİL; Ardicli, Sena; ARDIÇLI, SENA; Bursa Uludağ Üniversitesi/Veteriner Fakültesi.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı.; 0000-0001-6077-0478; 0000-0003-2758-5945; KHD-4075-2024; O-3394-2019; AAG-7421-2021; AAH-5294-2021
The aim of this study is to investigate cytological, microbiological and histopathological findings of the genital tracts in cows with different degree perineal conformation (PC) disorder. Totally, 28 cows brought the slaughterhouse were materials of the study. Information about to age, body weight, reproductive status, number of parturitions, days in milk (DIM) and the reason for slaughtering of the cows was obtained in the anamnesis learned from the owners. Before slaughtering, cows were evaluated in terms of PC and then they were divided into four groups with equal numbers of cows according to PC disorders: group I (GRI) normal; group II (GRII) mild; group III (GRIII) moderate and group IV (GRIV) severe. PC disorders of the cows were graded on a scale in terms of vulvar angle, vulvar length over ischial arch, depth of anus and perineal length. A body condition score (BCS) was also evaluated in the cows. The perineal region and vagina were inspected for vaginal discharge and vaginal mucosal appearance, respectively. Pneumovagina was classified as negative, suspicious and positive. Sterile swab samples were taken from vagina, cervix and uterus for bacteriological culture and identification. For cytological examination, smears were taken from vagina, cervix and uterus. Tissue samples were taken from vagina, cervix and uterus for histopathological examination. Statistically, Pearson's correlation, Fisher's exact tests and regression analysis were performed for all data. Clinically, characters of the vaginal discharge (serous, foamy, mucous, purulent and urine-mixed) and vaginal hyperemia increased in GRIII and GRIV. Microbiologically, as parallel to the PC disorder, E. coli in Enterobacteriaceae family was the most common bacterium in Group III and Group IV. As PC disorder in the groups increased, cytological examination findings were found to be significant, similar to clinical and microbiological examination. However, histopathological examination gave more meaningful results in groups. Statistic results pointed out that difference between the groups in terms of vaginal mucosa color, cervical and uterine microbiology was significant. In conclusion, PC disorder in cows constitutes predisposition for many genital canal diseases that may be the cause of infertility. The presence of genital canal diseases can be suspected in cattle using the PC scale. Therefore, it is appropriate to evaluate the health status of the genital canal with other diagnostic methods (microbiology, cytology and histopathology), especially in cows with PC disorder.
Determination of the alterations in quality parameters and consumer preference of dry-aged beef based on different periods of ageing using a purposive incubator
(Hellenic Veterinary Medical Soc, 2021-01-01) Üstüner, Hatice; Ardıçlı, Sena; Arslan, O.; Üstüner, Hatice; ARDIÇLI, SENA; Arslan, O.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Zootekni Anabilim Dalı; O-3394-2019; GRY-9670-2022; O-3394-2019; JHO-8192-2023
The aim of this study was to evaluate beef quality traits including pH, water holding capacity, cooking loss, meat colour, and Warner-Bratzler shear force in dry-aged beef and to determine the most favorable ageing time based on beef quality parameters and consumer acceptability. In this respect, longissimus dorsi samples were obtained from Simmental bulls and stored for an ageing period up to 21-days, and thus, the beef quality evaluation was performed at 0-day, 7-day, 14-day, and 21-day of the experimental period. Results revealed that the lowest shear force value was observed in beef samples on day-14 whereas the lowest pH value was determined in samples on day 21. Moreover, water holding capacity and beef colour values were significantly differentiated based on dry-ageing (P<0.05). In sensory panel evaluation, a significant difference is found only in meat colour rating (P<0.05). There was no significant difference between ageing periods and cooking loss. The most important technical point is that increasing dry-ageing time from 14 to 21 days did not desirably affect quality traits and sensory scores. Hence, dry-ageing for 14 days seemed to be the most economically efficient application. Taken altogether, the present results suggest that the potential for use of dry-ageing should be considered as an alternative method to produce high-quality beef with respect to the optimum ageing process.
Determination the effect of CSN1S1, CSN3 and AGPAT6 genes and lactation rank on physicochemical properties of goat milk
(Sivar-soc Italiana Veterinari Animali Reddito, 2020-08-01) Deniz, Dincel; DİNÇEL, DENİZ; Sena, Ardicli; ARDIÇLI, SENA; Hale, Samli; ŞAMLI, HALE; Faruk, Balci; BALCI, ÖMER FARUK; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı.; 0000-0002-8015-9032; 0000-0003-2758-5945; AAH-6192-2021; O-3394-2019
The physiochemical properties of milk are important factors in terms of the process of dairy production, quality, and profitability. However, the knowledge about the effect of genetic factors such as CSN1S1, CSN3, and AGPAT6 genes on these traits is insufficient. The objective of this study was to determine the effect of these genes and lactation rank (LR) on total acidity, citric acid, density (density), free fatty acid (FFA), freezing point degree (FPD) and urea parameters. A total of fifty (n=50) Saanen goats, which is known as the highest milk-producing breed within the goats were genotyped using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) methods. For genetic analysis, the blood samples were taken from the jugular vein by aseptic conditions. The DNA isolations were performed by the phenol-chloroform method from blood samples. Milk samples were collected during the lactation and evaluated by Fourier transform infrared (FTIR) spectroscopy for the physicochemical properties of Saanen goat milk. The statistical analysis was carried out using the least-squares of the GLM procedures. The results indicated that no significant differences were confirmed between the investigated genes with the physicochemical properties of goat milk. However, the effect of the AGPAT6 gene on density and the effect of LR on citric acid composition was found tended to be significant. Moreover, we determined the significant correlations between the physicochemical properties in the present study. According to the results, acidity was correlated with the citric acid (-0.304), density (0.641), FFA (-0.332) significantly. Other significant correlations were observed between the citric acid and FPD (0.275), LR (-0.313); the density and FFA (-0.315), FPD (0.436); the urea and LR (-0.369). These results and novel correlations may be useful for future studies on evaluating the potential impact of AGPAT6 gene on these traits to achieve breeding and commercial goals in the goat production and dairy industry.
Dietary intake of Spirulina platensis alters HSP70 gene expression profiles in the brain of rats in an experimental model of mixed stress
(Indian Acad Sciences, 2022-10-26) Seyidoğlu, Nilay; ARDIÇLI, SENA; Ardıçlı, Sena; Gurbanli, Rovshan; Köseli, Eda; Aydin, Cenk; AYDIN, CENK; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/; 0000-0003-2758-5945; 0000-0002-4812-4024; 0000-0002-3090-0099; KIC-9651-2024; O-3394-2019; JTT-0803-2023; AAS-2344-2020; GRJ-2026-2022
Spirulina platensis has gradually gained more attention for its therapeutic, antioxidant, and anti-inflammatory potential worldwide. However, the current molecular knowledge about the effects of spirulina on stress-related genes is rather limited. The effects of dietary intake of spirulina on the HSP70 gene expression were assessed in a controlled in vivo experimental design. Moreover, alterations in serum corticosterone levels, IL-2, IL-4, IFN-gamma, triglyceride, ALT, AST, relative gene expression values, and the correlations between them were evaluated. A total of 36 rats were divided into four groups: control group, stress-only group, spirulina group, and spirulina+stress group. To control the dose administration, S. platensis was applied by a gastric gavage in stress groups. Crowded environment stress and hosting alone stress were applied to the stress-only group and spirulina + stress group. RNA was extracted from brain samples using TRlpure and the relative gene expression assessment was performed using Roche-LightCycler-480-II real-time PCR-System. Gene expression values were remarkably different among the four experimental groups. The differences between stress-only and the spirulina groups were statistically significant (P<0.05). The correlation between the HSP70 gene expression and the IFN-gamma was found to be statistically significant (P<0.05; r=0.50). Results indicate a novel effect of spirulina on the HSP70 expression related to the stress-response. Data presented in this study may be useful for further studies to define not only the molecular genetic aspects through dietary S. platensis but also the effects of spirulina on stress-response and animal welfare.
Evaluation of candidate gene effects and environmental factors on reproductive performance of holstein cows
(South African Journal Of Animal Sciences, 2019-01-01) Ardıçlı, Sena; ARDIÇLI, SENA; Şamlı, Hale; ŞAMLI, HALE; Dinçel, Deniz; DİNÇEL, DENİZ; Soyudal, B.; Balcı, F.; BALCI, ÖMER FARUK; Bursa Uludağ Üniversitesi/Veteriner Fakültesi; 0000-0003-2758-5945; 0000-0002-8015-9032; 0000-0003-2382-1330; O-3394-2019; AAH-6488-2021; AAH-6192-2021
This study investigated the impact of nine polymorphisms located in the CSN2, CSN3, CSN1S1, CSN1S2, and parity on reproductive traits. The analysis was conducted on 165 Holstein-Friesian cows. Genotypes were identified using PCR-RFLP. The data of reproductive traits for four lactations were evaluated. Statistical analysis was carried out using least squares of the GLM procedures. Results indicated that CSN2 had significant effects on days before first insemination and first insemination to pregnancy interval. The SNP at the CSN3 was significantly associated with gestation length. A novel effect of OLR1-C223A on age at first calving was observed in the present study. Moreover, DGAT1 and LGB markers were significantly associated with calving interval and days before first insemination, respectively. In addition, significant environmental effects were as follows: calving year with days before first oestrus, days open, and first insemination to pregnancy interval; season with days open, first insemination to pregnancy interval, and calving interval; parity with days before first oestrus and days open. The present results and novel associations may therefore be useful and indicative for future studies on a genetic basis of cattle reproduction traits.
Evaluation of some environmental and genetic factors csn3 and agpat6 gene on milk yield and composition in saanen goats
(Univ Zagreb Vet Faculty, 2021-01-01) Dincel, Deniz; DİNÇEL, DENİZ; Ardicli, Sena; ARDIÇLI, SENA; Samli, Hale; ŞAMLI, HALE; Ogan, M. Mustafa; Balci, Faruk; BALCI, ÖMER FARUK; Bursa Uludağ Üniversitesi/Veteriner Fakültesi.; 0000-0002-8015-9032; 0000-0003-2758-5945; 0000-0003-2382-1330; AAH-6192-2021; O-3394-2019
This study was designed to determine the effect of some environmental factors on milk yield and composition in Saanen goats and the effect of CSN3 and AGPAT6 gene polymorphisms on milk production traits. Saanen goats were genotyped for milk traits, and the samples were collected during the lactation, and the milk yield/compositions of each goat were specified. In terms of the CSN3 and AGPAT6 genes, the genotypes were defined by PCR-RFLP. The milk yield (MY), lactation length (LL), protein, fat, total solid (TS), solid not fat (SNF), casein, lactose rates and yields were as follows; 388.9 +/- 17.5 kg, 243 +/- 5.81 days, 3.29 +/- 0.05%, 3.59 +/- 0.07%, 11.60 +/- 0.13%, 8.43 +/- 0.06%, 2.57 +/- 0.04%, 4.31 +/- 0.03%, 11.21 +/- 0.47 kg, 12.79 +/- 0.64 kg, 40.95 +/- 1.82 kg, 29.53 +/- 1.31 kg, 8.82 +/- 0.37 kg, 15.40 +/- 0.74 kg respectively. The determined genotype and allele frequencies of CSN3 and AGPAT6 gene were as follows; FF (%99.20), MF (%0.80), F (0.996), M (0.004); GG (%12), GC (%43.20), CC (%44.80), G (0.336), C (0.664). The impacts of lactation length on all milk yield parameters (protein, fat, TS, SNF, casein, lactose. MY) (P<0.001); the age on all milk yield parameters (P<0.001) and the protein, fat, SNF, casein rates (P<0.05), the TS rate and LL (P<0.01) were found to be significant. The birth type was not found to significantly affect any milk yield parameters. Although Saanen goats showed the variation for the AGPAT6 gene (g.9263C>G), the herd was found to be monomorphic (FF) for the CSN3 gene. The effect of the AGPAT6 gene on milk traits in Saanen goats was determined not to be statistically significant. Environmental factors, such as maternal age and lactation length, were found to significantly affect some milk traits in Saanen goats. So consideration of factors such as maternal age or lactation length could be useful for improving breeding strategies for dairy goats.
Fattening performance and carcass traits of imported simmental bulls at different initial fattening age
(Sivar-soc Italiana Veterinari Animali Reddito, 2020-08-01) Ustuner, Hakan; ÜSTÜNER, HAKAN; Ardicli, Sena; ARDIÇLI, SENA; Arslan, Oznur; Brav, Fahir Cankat; Bursa Uludağ Üniversitesi/Veteriner Fakültesi; 0000-0003-2758-5945; O-3394-2019; AAG-9127-2021
Fattening performance is one of the most important traits in cattle breeding. These traits are closely associated with adequate initial weights and slaughter endpoints. In this context, the aim of this study was to investigate optimum initial fattening age of imported Simmental young bulls and to provide a detailed analysis of initial fattening age effects on fattening performance and carcass characteristics. Seventy five animals were purchased from a single commercial farm where they had been reared under identical production conditions and were allocated into four groups according to age and live-weight as follows: Group I (4 months of age), Group II (6 months of age), Group III (8 months of age), and Group IV (10 months of age). All animals were housed in semi-open pens and were fed ad libitum with the same appropriate diet for 12 months. The animals were slaughtered in a commercial abattoir according to standard routines and carcass traits including hot and chilled carcass weights, dressing percentage, and chiling loss were determined. Morever, performance traits including total weight gain, feed conversion rate, average daily weight gain, and dry matter intake were estimated. Results revealed that the initial fattening age of bulls showed significant effects on total weight gain and chilled carcass dressing. In this context, Group I was characterized by the highest total weight gain, whereas, Group IV had the highest values for chilled carcass dressing. During early fattening periods, first three periods, average daily weight gain was significantly different among the treatments. This study pointed out that, concerning importation, younger Simmentals with the initial fattening age of four months may be more suitable and profitable for medium-term fattening. The present results may have a potential to influence adequate management practices in Simmental farms, and moreover, the recent strategies for importation of cattle.
Gender determination by PCR assay for the sex-determining region Y(SRY) gene amplification in linnaeus’s two-toed sloth (Choloepus Didactylus)
(Bursa Uludağ Üniversitesi, 2023-06-15) Bozkurt, Berkay; Vatansever, Ezgi; ARDIÇLI, SENA; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı; 0000-0003-2758-5945
In Linnaeus’s two-toed sloths (Choloepus didactylus), there is no distinct sexual dimorphism. It is an obstacle for gender determination fromthe external genitalia, especially in newborns or young sloths. Hence, easy, rapid, and reliable genetics-based methods for gender identificationof the sloths are needed to continue captive breeding more successfully. In this study, a PCR-based technique that allows gender determinationof two-toed sloths by using a sex-determining region Y (SRY) gene marker was described. The hair samples from young (suspectgender) and adult sloths (known gender) were used in genetic analysis. Initially, genomic DNA was isolated from hair root samples using Roche high pure PCR template preparation kit. The SRY primers were specifically designed based on the NCBI and Ensembl databases, andthey were verified with the BLAST program concerning the two-toed sloth genome. PCR amplification with the SRY-specific primers wascarried out by a programmable thermal cycler device using FastStart High Fidelity PCR System, Roche dNTPack. The samples were then electrophoresedon 2% agarose gels and were visualized by a gel documentation and analysis system. A specific band in the electrophoresis patternis diagnostic for a male individual with a partial SRY region. Hence, the analysis demonstrated that the samples belonged to a male two-toedsloth. Two-toed sloth species are commonly preferred animals in zoos. Gender determination is inevitable for these animals in captivity tobe raised successfully and healthily. Molecular genetic techniques allow high efficiency in taxonomic evaluations and gender identification inspecies that do not display sexual dimorphism. The PCR assay described here may be helpful for a rapid genetic analysis that can be widelyused in gender determination for two-toed sloths.
Genetic variation in the bovine myogenic determination factor 1 (g.782g> A polymorphism) and its influence on carcass traits in Turkish grey steppe cattle
(TUBİTAK, 2021-01-01) Ardıçlı, Sena; Çobanoglu, Özden; ARDIÇLI, SENA; ÇOBANOĞLU, ÖZDEN; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı.; 0000-0003-2758-5945; O-3394-2019
MyoD1 gene is a member of the myogenic differentiation gene family, which plays a crucial role in growth and development. There is a lack of information about the MyoD1 variants and their phenotypic influences in Turkish Grey Steppe cattle. Therefore the present study aimed to determine the genetic variability of MyoD1 g.782G>A polymorphism and to evaluate its effects on carcass characteristics in Turkish Grey Steppe cattle. Analyses were conducted on a total of 142 Turkish Grey Steppe bulls. The PCR-RFLP technique was used for genotyping of the g.782G>A polymorphism in exon 1 of the MyoD1 gene. The population genetic parameters including compatibility with Hardy-Weinberg equilibrium, heterozygosity, number of effective alleles, the polymorphism information content, and the fixation index were calculated. Statistical analysis was carried out using the least square methods of the general linear model procedure. Results revealed that BB was the preponderant genotype (41.55%) but the AA genotype exhibited a remarkable close frequency (39.44%). Accordingly, allelic frequencies were very close to each other (A:0.49; B:0.51). The chi 2 test revealed that the corresponding MyoD1 locus did not conform to Hardy-Weinberg equilibrium (p < 0.001). Heterozygosity, number of effective alleles, and the polymorphism information content values were 0.4998, 1.9992, and 0.3749, respectively. Moreover, the fixation index value was found to be 0.6195. These results indicated that MyoD1 g.782G>A polymorphism is a mildly informative genetic marker for Turkish Grey Steppe cattle. Statistical analysis indicated that the studied MyoD1 locus was significantly associated with chilling loss, carcass bone content, and carcass length (p < 0.05). The AA genotype was characterized by a higher chilling loss percentage but lower carcass length and bone content compared to BB and heterozygous genotypes. This study may provide valuable information regarding cattle carcass assessment and improvement through marker-assisted selection.
The association between the STAT1 g.3141C>T polymorphism and reproductive performance in high-yielding holstein-friesian dairy cows
(Sivar-soc Italiana Veterinari Animali Reddito, 2023-04-01) Güçlü, Şevket; Dursun, Serdar; Kocaer, İsmail İlker; GÜMEN, AHMET; Aldevir, Özgür; Gümen, Ahmet; ARDIÇLI, SENA; Ardıçlı, Sena; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi.; 0000-0002-3607-4365; 0000-0003-2758-5945; O-3394-2019
In dairy cattle, selection programs have mainly focused on high milk production which led to significant improvements in yield. However, it has also caused serious problems in bovine fertility. Reproductive performance is increasing in popularity worldwide. Therefore, this study aimed to evaluate the effects of g.3141C>T polymorphism of bovine STAT1 gene on reproductive traits in high-yielding Holstein-Friesian cows. The data of 4800 cows were used and the initial experimental population consisted of 500 purebred cows housed in three free-stall barns. All animals were fed the same diets and had the same management procedures. The phenotypic traits analyzed in this study were total milk yield, 305-day milk yield, days open, the number of inseminations, and culling rates based on repeat-breeding. Body condition scores, lactation season, and lactation rank were also evaluated in statistical models. Initially, all of the cows were ranked by a selection index based on individual milk yield records and health traits. Next, a total of 75 cows were selected and genotyped for the STAT1 marker located in 3'UTR by the PCR-RFLP method. Genotype-phenotype association analysis was carried out by the least-squares method as applied in a general linear model (GLM) procedure with Tukey's test as a post-hoc comparison. The association between the cull rates and the genotypes was evaluated by Pearson's chi-square test. Population genetics parameters including heterozygosity (He), homozygosity (Ho), number of effective alleles (Ne), and the polymorphic information content (PIC) were evaluated and the deviation from Hardy-Weinberg Equilibrium (HWE) was tested. Results revealed that g.3141C>T polymorphism exhibited admissible levels of population parameters (He=0.4801; Ne=1.9231) indicating that this marker is moderately informative for the selected population (PIC=0.3648). There was a deviation from HWE (P<0.001). In GLM, the association between the STAT1 marker and the number of inseminations was found to be statistically significant (P<0.05). The TT animals were characterized by the highest number of inseminations (3.71 +/- 0.73). On the other hand, heterozygous animals were shown to be associated with desirable reproduction performance. This is a critical result because the STAT1 g.3141C>T marker is included in many SNP-panels or SNP-chips for its previously reported effects on milk yield. To the best of our knowledge, this study has shown a novel effect of this STAT1 marker on the number of inseminations per conception. Considering the TT genotype has a frequency of 26.67%, ignoring this association can lead to a significant reproduction performance decrease on a herd basis. Moreover, there was a significant association between the STAT1 and cull rates (P<0.01). There was no association between the STAT1 and any other traits analyzed. This study demonstrates novel effects of the STAT1 gene, and hence, may contribute to the adequate genotypic evaluation of dairy cattle reproduction performance.
The effects of calcineurin inhibitors on sperm dna fragmentation in male kidney transplant recipients
(Oxford Univ Press, 2015-05-01) Ersoy, Alparslan; Samli, Hale; Gul, Cuma Bulent; Samli, Murat; Ardicli, Sena; Balci, Faruk; ERSOY, ALPARSLAN; ŞAMLI, HALE; ARDIÇLI, SENA; BALCI, ÖMER FARUK; Uludağ Üniversitesi/Tıp Fakültesi/Nefroloji Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı.; 0000-0003-2758-5945; 0000-0003-2382-1330; AAH-6488-2021; O-3394-2019; AAH-5054-2021; I-5009-2019
The variation in the beta-casein genotypes and its effect on milk yield and genomic values in holstein-friesian cows
(Taylor & Francis Inc, 2023-10-12) Aksu, Emrah; Ardıçlı, Sena; ARDIÇLI, SENA; Aldevir, Özgür; Gümen, Ahmet; GÜMEN, AHMET; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Jinekoloji ve Doğum Anabilim Dalı.; 0000-0003-2758-5945; O-3394-2019
The A2 milk marker is gaining popularity worldwide; thus, many farms plan to convert their dairy cattle herds to the A2A2 genotype. Variation in beta-casein genotypes needs to be monitored in large dairy cattle populations. Therefore we aimed to evaluate the genotypic distributions, population genetics, and diversity parameters in Holstein-Friesian cows. A total of 1200 cattle were genotyped using the Affymetrix (R) Axiom (R) array system. We performed an association analysis regarding the CSN2 genotypes and phenotypic traits, including lactation and test-day milk yield. We next evaluated the effects of the genotypes considering the genetic merit of the animals. Animals were grouped based on their PTAs for milk production, fat, protein, and daughter pregnancy rate. Thus, we tested the genotype x genetic merit interaction for significance. The A2 allele frequency is remarkably high (0.68), and the heterozygous genotype is predominant (46.25%). The marker showed intermediate variability and diversity levels, indicating a considerable frequency of the A1A1 genotype (9.33%) remains in the population. ANOVA results showed no significant association between the CSN2 genotypes and milk yield traits. A similar finding is valid for the genotype x genetic merit regarding the genomic test results. The data presented here may be helpful for further investigations and applications on A2 milk production.