Browsing by Author "Ata, Nezih"
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Publication Associations between CYP17 gene polymorphisms, temperament and maternal behavior in ewes, and growth in their lambs(Elsevier Science, 2021-06-30) Karaca, Serhat; Şer, Gazel; Ülker, Hasan; Yılmaz, Onur; Çakmakcı, Cihan; Ata, Nezih; Sarıbey, Muammer; ÜLKER, HASAN; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Zootekni Bölümü.; JJB-9576-2023This study was conducted to determine temperament and maternal care classes, CYP17 gene polymor-phisms, and cortisol status of a group of Norduz ewes. Additionally, the relationships between these fac-tors, their effects on lambs survival and growth characteristics were investigated. A total of 72 ewes were subjected to arena and isolation box tests to determine their temperament classes. The distance the ewe retreated from the lamb during tagging was utilized to assess ewe maternal care classes. Ewes were genotyped for CYP17 628 SNP. Blood samples were collected between lambing and weaning to measure cortisol concentrations of ewes. The survival and growth data for the lambs of these ewes were recorded until weaning. Two temperament (low reactive and high reactive) and 2 maternal care classes (strong and moderate) were present. Two CYP 17 SNP 628 alleles (A and G) and 3 genotypes (AA, AG, and GG) were identified. For the distribution of CYP17 genotype frequencies within the population, the temperament or maternal care classes were not different (P > 0.05). The temperament of ewes did not affect mater-nal care in our flock. Positive partial correlations between cortisol and total cross, distance to stimulus, and sniffing were determined (P < 0.05). CYP17 genotype was an effective factor for some behavioral traits (i.e., total cross) and cortisol release (P < 0.001). Neither temperament (P = 0.636) nor maternal care classes (P = 0.985) differed with cortisol concentrations. CYP17 genotype, cortisol status, tempera-ment or maternal care class of ewes did not affect birth or weaning weight of their lambs (P > 0.05). Although there was no significant relationship among CYP17 genotype, maternal care, and lamb survival and growth, the effect of CYP17 genotype on some behavioral traits and cortisol release was found to be significant. Further studies with larger animal populations are required to elucidate the possible use of CYP17 SNP 628 genotypes as genetic markers for temperament classification.Item Associations between GH, PRL, STAT5A, OPN, PIT-1, LEP and FGF2 polymorphisms and fertility in holstein-friesian heifers(Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 2017) Yılmaz, Onur; Okut, Hayrettin; Ata, Nezih; Öner, Yasemin; Yılmazbaş, Mecitoğlu Gülnaz; Keskin, Abdulkadir; Uludağ Üniversitesi/Ziraat Fakültesi/Zooteknik, Biyometri ve Genetik Bölümü.; Uludağ Üniversitesi/Veteriner Fakültesi.; 0000-0002-2904-8986; 0000-0003-2817-3221; ABB-3181-2020; AAG-4440-2019; 9634793800; 36457964000; 24473229800In this study, it was aimed to investigate polymorphisms in seven genes (GH, PRL, STAT5A, OPN, PIT-1, LEP and FGF2) related to reproductive traits in dairy heifers. Frequency distributions of the genotypes between fertile and repeat breeder heifers groups were investigated. Allele effects on fertility were also analyzed. Blood samples were taken from a total of 160 Holstein-Friesian heifers and they were divided into two groups according to their artificial insemination numbers (AI). The heifers becoming pregnant after the first AI were used as the fertile heifers (FH, n= 80) and the heifers with 3 or more equal AIs were accepted as the repeat breeder heifers (RBH, n= 80). All the animals were genotyped by the PCR-RFLP method for seven genes and the association works were performed for 145 animals (RBH, n= 79; FH n= 66). For all loci investigated, two alleles and three genotypes were found for overall population with the exception that PRL locus had two alleles and two genotypes. The chi-square test (.2) revealed that the whole population and the two groups separately were at Hardy-Weinberg equilibrium. The genotype distributions of PIT-1 and STAT5A conspicuously differed between the FH and the RBH groups; however, these differences were not found significant. Association of GH-AB genotype was found significant on AI number for the first pregnancy. Mixed effect logistic regression model was used to investigate the allele effects on fertility. No linkage disequilibrium was detected between the investigated loci.Publication Genetic identification and characterisation of some Turkish sheep(Elsevier, 2021-06-14) Alarslan, Emre; Ata, Nezih; Yılmaz, Onur; Öner, Yasemin; Kaptan, Cüneyt; Savaş, Türker; Yılmaz, Alper; ÖNER, YASEMİN; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Zootekni Bölümü.; 0000-0002-2904-8986; ABB-3181-2020Turkey's biological diversity is considerable because of its biogeographical positions, therefore it is plentiful in terms of diversity in animal genetic resources. Genetic diversity is important in sustainable breeding and conservation programs in livestock. The aim of this study was to determine the genetic structure of Yalova genotype, Kivircik, Tahirova and Es,me sheep breed by using twenty microsatellite markers. A total of 223 Turkish native unrelated sheep (Yalova, Kivircik, Tahirova and Es,me) which were raised in sixteen flocks located in four different provinces (Kirklareli, canakkale, Yalova ve Us,ak) were investigated. A total of 543 alleles were found. The number of loci showed deviations from Hardy-Weinberg equilibrium ranged from 11 (Kivircik) to 17 (Yalova). Although a total of 183 private alleles were detected, only 21 of them had allele frequency above 0.05. The number of observed alleles differed from 42 (for OarFCB20 and ILSTS5) to 20 (for OarCP34 and DRBP1). The highest Ne and PIC values (19.23 and 0.95, respectively) were also observed for ILSTS5 locus. While expected heterozygosity (He) ranged between 0.83-0.95, observed heterozygosity differed from 0.69-0.95. These results can be used for the initiation of registration studies for Yalova after combining phenotypic data evaluations.Publication Genome-wide association studies of preweaning growth and in vivo carcass composition traits in esme sheep(Wiley, 2021-07-22) Yılmaz, Onur; Kızılaslan, Mehmet; Arzık, Yunus; Behrem, Sedat; Ata, Nezih; Karaca, Orhan; Elmacı, Cengiz; Cemal, İbrahim; ELMACI, CENGİZ; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Zootekni ve Hayvan Besleme Bölümü.; 0000-0003-4819-0221; V-7882-2019Sheep are considered as a major contributor of global food security. Moreover, sheep preweaning growth traits as well as in vivo carcass composition traits such as ultrasonic measurements of Longissimus dorsi muscle depth (UMD) and back-fat thickness (UFD) are crucially important indicators of meat yield and hot carcass composition. Despite their relative importance for productivity and profitability of a sheep production system, detected QTL for these traits are quite scarce. Therefore, we implemented GWAS for these traits using animal mixed model-based association approach provided by GenABEL in Esme sheep. Three genome-wide and 14 individual chromosome-wide associated SNPs were discovered. As a result, ESRP1, LOC105613082, ZNF641, DUSP5, TEAD1, SMOX, PTPRT, RALYL, POM121C, PHIP, LOC101106051, ZIM3, PEG3, TRPC7, FBXL4, LOC105610397, LOC105616489 and DNAAF2 were suggested as candidates. Some of the discovered genes and involved pathways were already annotated to contribute growth and development in various species including human, mice and cattle. All in all, the results of this study are expected to strongly contribute to shed a light on the underlying molecular mechanisms behind growth and carcass composition traits, with potential implications on studies aiming faster genetic improvement, targeted low-resolution SNP panel designs and genome-editing studies.