Browsing by Author "Bagheri, Abdolreza"
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Item Biochemical changes and SDS-PAGE analyses of chickpea (cicer arietinum L.) genotypes in response to salinity during the early stages of seedling growth(Uludağ Üniversitesi, 2014-07-04) Arefian, Mohammad; Vessal, Saeedreza; Bagheri, AbdolrezaSalinity is one of the most serious abiotic stresses for plants, causing other subsequent consequences such as oxidative stress, which eventually leads to cell death. Measured various biochemical parameters in chickpea genotypes were performed under various NaCl concentrations (0, 8 and 12 dS.m-1 ( in controlled condition at 21 and 28-day after sowing (DAS). After determination of tolerant (MCC544) and susceptible (MCC806) genotypes and also the best differential salt concentration, SDS-PAGE was used to compare protein profiling in these two genotypes in 3 time points with four replicates. Proline and protein contents were significantly higher in MCC544 as much as 27 fold (for proline) and 30% (for protein) increase over control in 28 DAS at 12 dS.m-1 of salt. The leaf soluble carbohydrates increased significantly in MCC544 and MCC760, compared with others. The minimum decline of electrolyte leakages (6%) and malondialdehyde (MDA) content was belonged to MCC760 while MCC806 genotype showed the highest decrease rate (more than 20%). Total leaf chlorophyll content decreased in all genotypes. More strong and positive correlations between parameters was recorded in tolerant genotypes which resulted in membrane and osmotic balance. Analyses of SDS-PAGE revealed that more rapid accumulation and/or less degradation of proteins occurred in higher molecular weight proteins. Moreover, the response of genotypes through protein changes before 96 h stress might be a possible reason for salinity tolerance in this condition.Item Disinfecting effects of nano silver fluids in gerbera (Gerbera jamesonii) capitulum tissue culture(Uludağ Üniversitesi, 2012) Fakhrfeshani, Masoud; Bagheri, Abdolreza; Sharifi, AhmadMicrobial contamination is one of the most important problems in plant tissue culture and various methods are employed to reduce it. Microbial contamination is an important barrier in the development of Gerbera tissue culture and micropropagation procedure even after disinfecting with normal methods. In this experiment the antifungal and antibacterial activity of nano-silver fluids was investigated in gerbera tissue culture and the effects of four different nano-silver concentration (25, 50, 100 and 200mg L-1) in four soaking time of explants (15, 30, 60 and 180 min) were compared with two control treatments that included disinfecting explants with soaking them in Sodium Hypochlorite and Sodium Hypochlorite followed by Mercury Chloride aqueous solution. The explants were cultured on dedicated MS medium to evaluate the effects of NS on viability and other apparent properties. The analysis of variance resulted that there are significant differences in contamination rate, both among treatments and between control and treatments. The 200 mg L-1 nano-silver solution had successfully controlled bacterial and fungal contamination and had no undesired effects on regeneration of plantlets. According to reports on high levels of contamination in Gerbera tissue culture and also the hazardous environmental effects of mercury chloride, so nano-silver solution can be used as a low risk fungicide and bactericide in Gerbera capitulum tissue culture.Item In vitro culture of carnation (Dianthus caryophyllus L.) focusing on the problem of vitrification(Uludağ Üniversitesi, 2011) Kharrazi, Mahdiyeh; Nemati, Hossein; Tehranifar, Ali; Bagheri, Abdolreza; Sharifi, AhmadThis study was conducted to evaluate the effect of plant growth regulators on in vitro shoot multiplication, vitrification and rooting of two carnation cultivars (Eskimo Mogr and Innove Orange Bogr). Isolated axillary buds were cultured on MS medium supplemented with different levels of Benzyl amino purine (BAP) or kinetin (Kin) in combination with 0.2 mg/l NAA and shoot multiplication and vitrification rate were tested. Then different concentrations of Naphthalene acetic acid (NAA) or Indole butyric acid (IBA) on root induction of regenerated shoots were surveyed. Shoot regeneration and vitrification were influenced by the cultivar, kind of cytokinin and its concentration. Eskimo Mogr produced more shoots in comparison with Innove Orange Bogr (6.17 and 3.83 shoots/explant, respectively). BAP-containing media had more shoot multiplication and vitrification than Kin and by increasing the concentration of cytokinin, shoot multiplication and vitrification increased. There was a negative correlation between plant height and internode length with vitrification (-77% and -85%, respectively). By considering the amount of multiplication and rate of vitrification for obtaining the highest number of normal shoots, MS medium containing lower concentration of BAP (1mg/l for Eskimo Mogr and 2 mg/l for Innove Orange Bogr) and 0.2 mg/l NAA is recommended. Rooting of regenerated shoots was dependent on the cultivar. The highest root percentage for Eskimo Mogr (83%) was achieved on the medium supplemented with 0.5 mg/l NAA, while for Innove Orange Bogr (98%) it was obtained on the medium containing 1.5 mg/l IBA.