Browsing by Author "Berhow, Mark A."
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Item Effects of glucosinolates and their hydrolysis products on biochemical and performance parameters in broiler chicken diets(Indian Council of Agricultural Research, 2016-03-16) Berhow, Mark Alan; Belenli, Deniz; Polat, Ümit; Orman, Abdulkadir; Yesilbağ, Derya; Berhow, Mark A.; Uludağ Üniversitesi/Veteriner Fakültesi/Biyokimya Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Hayvancılık Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Hayvan Besleme ve Beslenme Hastalıkları Anabilim Dalı.; 0000-0001-9138-4422; AAG-9134-2021; AAK-5370-2020; 56743270100; 56235316900; 24335834100; 9246817500; 7003305843Glucosinolates are important bioactive molecules and widely found in Brassicaceae species (cress, brussels sprouts, mustard, broccoli, kale etc.). Depending on the amount of these vegetables consumed, both positive and negative metabolic effects from glucosinolate metabolites may occur. The aim of this study was to investigate inexpensive animal food sources that both increase weight gain and provide enhanced performance parameters without adversely affecting the animal's health and metabolism; to evaluate dose adjustment of food containing glucosinolates in animals; and to evaluate changes in the biochemical and performance status of chickens on the glucosinolate containing diets. Day-old Ross 308 broiler line chicks (624) were divided into 1 control and 3 treatment groups. Cress seed (Lepidium sativum) was added 0.05% for the first treatment group (group 1, 10g/kg), 0.10% for the second treatment group (group 2, 20g/kg) and 0.15% for the last treatment groups (group 3, 30 g/kg) to the diet. Serum samples were evaluated for serum glucose, adiponectin, leptin, growth hormone, estradiol and cortisol levels. Feed intake, live body weight gain and feed conversion ratio were investigated for performance parameters. The results showed that dietary cress seed supplementation as feed additive (10, 20 and 30 g/kg) did not significantly improve the dietary performance, or carcass parameters of broiler chickens. Feed intake was the highest in group 2 (20g/kg), female live weight was the highest in group 2 (20 g/kg) and 3 (30 g/kg).Item Effects of glucosinolates and their hydrolysis products on energy balances and performance parameters and histological parameters in broiler chicken diets(Bursa Uludağ Üniversitesi, 2018-07-17) Belenli, Deniz; Berhow, Mark A.; Polat, Umit; Erdost, Hatice; İlhan, Tuncay; Orman, Abdülkadir; Yesilbağ, Derya; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Biyokimya Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Hayvancılık Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Hayvan Besleme ve Beslenme Hastalıkları Anabilim Dalı.Glucosinolates are important bioactive molecules and widely found in Brassicaceae species (cress, brussels sprouts, mustard, broccoli, kale etc.). Depending on the amount of these vegetables consumed, both positive and negative metabolic effects from glucosinolate metabolites may occur. The aims of this study were to; investigate inexpensive animal food sources that both increases weight gain and provides enhanced performance parameters without adversely affecting the animal’s health and metabolism; to evaluate dose adjustment of food containing glucosinolates in animals; and to evaluate changes in the biochemical and performance status of chickens on these glucosinolate containing diets. A total of 624 one-day-old Ross 308 broiler line chicks were divided into one control and three treatment groups. Cress seed (Lepidium sativum) was added 0.05% for the first treatment groups (Group 1, 10 g/kg), 0.10% for the second treatment groups (Group 2, 20 g/kg) and 0.15% for the last treatment groups (Group 3, 30 g/kg) to the diet. Serum samples were evaluated for serum glucose, adiponectin, leptin, growth hormone, estradiol and cortisol levels. Performance parameters investigated included feed intake, live body weight and feed conversion ratio. . The villus length, number of goblet cells, crypt depth were determined for histological analyses. According to histological results, villus length was significant at p < 0.05 level between control and group 1 and at p < 0.001 level with groups 2 and 3 at 21 days. The depth of the crypts belonging to the control and experimental groups was not significant between the control group and the group 1 when the statistic was evaluated on the 21st day, whereas between the control group and the group 2, p < 0.05 and p < 0.01; statistical significance was found at p < 0.001 level between group 3 and control group. The results showed that dietary glucosinolate supplementation as feed additive (10, 20 and 30 g/kg) did not significantly improve the dietary performance, or carcass parameters of broiler chickens. Feed intake was the highest in group 2 (20 g/kg), female live weight was the highest in group 2 (20 g/kg) and 3 (30 g/kg). In conclusion, the rates of the cress seed (0.05, 0.10 and 0.15%) that contain glucotropaeolin were not affected for feed additive on performance (especially live weight and live weight gain) and carcass parameters.Item Optimized analysis and quantification of glucosinolates from Camelina sativa seeds by reverse-phase liquid chromatography(Elsevier, 2013-04) Berhow, Mark A.; Glinski, Jan A.; Glensk, Michal; Vaughn, Steven F.; Isbell, Terry; Ayala-Diaz, Ivan; Marek, Laura; Gardner, Candice; Polat, Ümit; Uludağ Üniversitesi/Veterinerlik Fakültesi/Biyokimya Anabilim Dalı.; 56235316900Gold-of-pleasure or false flax (Camelina sativa L Crantz) is being developed as an alternative oil crop for biodiesel and for food use. The seed meal, which contains three relatively unique glucosinolates, is being evaluated for approval for use as an ingredient in animal feeds and for other uses. The objective of this research was to develop reproducible methods for the isolation of large quantities of pure camelina glucosinolates (glucoarabin, glucocamelinin, and 11-(methylsulfinyl)-undecylglucosinolate) and develop efficient methods for quantifying these compounds. The separation and purification of the camelina glucosinolates were achieved using a combination of reverse phase chromatography, counter-current chromatography and ion exchange chromatography. An efficient reverse phase HPLC separation method was used to quantitate the glucosinolate content in camelina seed and plant extracts. The quanti ration methodology was used to measure glucosinolate levels in seeds from 30 cultivars grown in a U.S. field trial and measure glucosinolates levels in sprouted camelina seeds.