Browsing by Author "Cerón, José Joaquín"
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Item Changes in salivary analytes in canine parvovirus: A high-resolution quantitative proteomic study(Elsevier, 2018-09-18) Franco-Martínez, Lorena; Tvarijonaviciute, Asta; Horvatić, Anita; Guillemin, Nicolas; Cerón, José Joaquín; Escribano, Damián; Eckersall, David; Lamy, Elsa; Martínez-Subiela, Silvia; Mrljak, Vladimir; Kocatürk, Meriç; Yılmaz, Zeki; Uludağ Üniversitesi/Veterinerlik Fakültesi/Dahiliye Anabilim Dalı.; 0000-0001-9836-0749; V-5578-2017; 36437200800; 35944810500The present study evaluated the changes in salivary proteome in parvoviral enteritis (PVE) in dogs through a high-throughput quantitative proteomic analysis. Saliva samples from healthy dogs and dogs with severe parvovirosis that survived or perished due to the disease were analysed and compared by Tandem Mass Tags (TMT) analysis. Proteomic analysis quantified 1516 peptides, and 287 (corresponding to 190 proteins) showed significantly different abundances between studied groups. Ten proteins were observed to change significantly between dogs that survived or perished due to PVE. Bioinformatics' analysis revealed that saliva reflects the involvement of different pathways in PVE such as catalytic activity and binding, and indicates antimicrobial humoral response as a pathway with a major role in the development of the disease. These results indicate that saliva proteins reflect physiopathological changes that occur in PVE and could be a potential source of biomarkers for this disease.Item Serum choline and butyrylcholinesterase changes in response to endotoxin in calves receiving intravenous choline administration(Elsevier, 2019-07-09) Eralp İnan, Oya; Tvarijonaviciute, Asta; Rubio, Camila Peres; Cerón, José Joaquín; Ulus, Ismail Hakki; Kocatürk, Meriç; Kasap, Sevim C.; Cansev, Mehmet; Yılmaz, Zeki; Uludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı; Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji Anabilim Dalı.; 0000-0002-2849-1222; 0000-0001-9836-0749; V-5578-2017; AAP-7998-2020; M-9071-2019; A-9637-2008; 36437200800; 36514844800; 8872816100; 35944810500Endotoxemia treatment options are still of interest due to high mortality and choline treatment is one of them because of its role in the cholinergic anti-inflammatory pathway. This study investigated serum choline and butyrylcholinesterase (BChE) responses, and their correlations with inflammatory, oxidative stress and tissue damage biomarkers, including paraoxanase-1 (PON1), and clinical signs in calves with endotoxemia and the effect of choline treatment in these responses. Healthy calves (n = 20) were divided equally into 4 groups: Control (0.9% NaCl, iv), Choline (C; 1 mg/kg/iv,once), Lipopolysaccharide (LPS; 2 mu g/kg/iv,once) and LPS + C. Clinical and laboratory examinations were performed before and 0.5-48 h (hrs) after treatments. Following LPS administration, serum choline level increased at 0.5-24 h (P < .01), whereas serum BChE and PON1 level decreased at 48 h (P < .01) compared to their baselines. In LPS + C group, the increase in serum choline level was significantly higher (P < .01) than that of C and LPS groups. LPS did not decrease serum BChE levels significantly in calves treated with choline. Serum choline and BChE results correlated negatively with white blood cell count and positively (P < .001) with PON1 levels, oxidative stress index, inflammation and hepato-muscular injury markers. In conclusion serum choline and BChE may have a role in the pathophysiology of endotoxemia in calves. High serum choline concentration is associated with an improvement in response to LPS administration in calves treated with choline, probably by preventing the imbalances between oxidative stress and anti-oxidant capacity, preventing the serum BChE and PON1 decreases, and inhibition/attenuation of acute phase reaction and hepato-muscular injury in calves with endotoxemia.