Browsing by Author "Ibegbu, Augustine Oseloka"
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Item The effects of hypoxia and opioid receptor agonists treatment in cortical B50 neuronal cells in culture(Uludağ Üniversitesi, 2012-12-06) Ibegbu, Augustine Oseloka; Fyfe, Lorna; McBean, Douglas; Mullaney, IanHypoxia has been implicated in nerve cell deaths in many neurological disorders and opioid receptor agonists have some positive benefits on the nervous system. The aim of the present work was to investigate the effects of hypoxia and opioid receptor agonists’ treatment on the morphology of B50 cells cultured in hypoxia using neuronal pattern and pattern formation as a case study. The B50 cells were cultured in normal incubator (21%O2; 5% CO2) as the control group and hypoxic incubator (5%O2; 5% CO2) as the experimental group and three opioid receptor agonists namely DAMGO (µ), DSLET (δ) and ICI-199,441 (κ) were administered to the cells for 48 hours as treatment against hypoxia after 48 hours of culture at 10µM, 50µM and 100µM concentrations. Neuronal morphology and wellbeing was assessed using same field morphological assessment and lactate dehydrogenase leakage (LDH). The result showed groups of dead and degenerating B50 neuronal cells, altered neuronal pattern and pattern formation and some significant changes (P<0.05) in cellular levels of LDH leakage in normal, hypoxic cells and cells treated with different agonists. The changes in morphology, neuronal pattern and LDH release indicate that hypoxia induced morphological and cellular changes in B50 cells in hypoxia and opioid agonists have some potential benefits in the treatment of hypoxia-induced changes in B50 cells in culture.Item Effects of vitamin e on phostoxin-ınduced changes in the liver and biochemical parameters of adult wistar rats(Uludağ Üniversitesi, 2013-11-25) Ibegbu, Augustine Oseloka; Alatise, Ahmad Tajudeen; Olaniyi, Babatunde Abdullateef; Samnan, Dassah Julcit; Emmanuel, Umana Uduak; Oliver, Hamman Wilson; Abraham, Musa Sunday; Steven, Akpulu PeterThe effects of antioxidant Vitamin E on phostoxin-induced changes in the liver and haemato-biochemical parameters in Wistar rats were studied. Thirty adult Wistar rats of both sexes were grouped into six groups of five rats each. Group 1 was the Control and was given normal saline, Group 2 was exposed to phostoxin for 3 hours with vitamin E and Group 3 was exposed to phostoxin for 1½ hours with vitamin E. Groups 4 and 5 were exposed to phostoxin for 3 hours and 1½ hours respectively and Group 6 was treated with vitamin E only. The rats were exposed to phostoxin through inhalational method for one week. At the end of the exposure period, the rats were sacrificed, the blood and tissues collected for analysis and procession for studies. The results showed increase in the body weight of the rats which could be as a result of the effect of phostoxin leading to increase in appetite of the rats. The results of biochemical parameters showed there was significant increase in AST and ALP (P<0.05), but the change in ALT in the serum of treated animals was not significant. The results from the histological observations of the Liver in the experimental rats showed changes in all the Groups that were treated with phostoxin of which the damage was dependent on the exposure time to phostoxin and Vitamin E may play a role as an antioxidant resulting in ameliorative effects in phostoxin-induced toxicity.