Browsing by Author "KIVANÇ, SERTAÇ ARGUN"

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Antibiofilm effect of probiotic lactic acid bacteria against bacillus spp obtained from the ocular surface
(Verduci Publisher, 2021-01-01) Akova, Berna; Kıvanç, Sertaç Argun; Kıvanç, Merih; AKOVA, BERNA; KIVANÇ, SERTAÇ ARGUN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Göz Hastalıkları Anabilim Dalı; 0000-0003-0995-5260; ABE-3033-2020; AAH-6518-2021
OBJECTIVE: Increasing emergence of antibiotic resistance has led to developing alternative methods to overcome this issue. The antibiotic resistance is mainly associated with formation of biofilms. Restoring healthy microbiota is one of these methods to fight the biofilm formation. In terms of this, the use of probiotics is a novel approach. In this study. we aimed at investigating the effect of exopolysaccharides (EPSs) of different lactic acid bacteria as probiotics on Bacillus spp isolated from the ocular surface, which is known to form biofilms.MATERIALS AND METHODS: Pathogenic microorganisms were cultivated in "Brain-Hearth infusion" (BHI) broth, and lactic acid bacteria were grown in "De Man, Rogosa. Sharpe" and M17 broth. Molecular identification of lactic acid bacteria was made according to the sequence information of the 16S rRNA gene region. Antimicrobial activity of lactic acid bacteria was determined by sandwich overlay method. The minimum inhibitory concentration values of the exopolysaccharides and antibiofilm activity were determined by microtitration method. For evaluating the effect of EPSs of probiotic bacteria on biofilm, the mean and standard deviations of optical density values were calculated.RESULTS: The most effective EPS against B. cereus was EPS from L. rhamnosus 24, followed by EPS from L. plantarum and L. acidophilus. The biofilm formation of all pathogenic bacteria that were exposed to probiotic bacteria except L. rhamnosus 621 and 622 and L. rhamnosus 3111 was lower than the biofilm formation of the control group.CONCLUSIONS: EPSs obtained from lactic acid bacteria have antibacterial and antibiofilm activity on pathogenic bacteria isolated from ocular surface. This study is one of the pioneer studies in restoring healthy microbiota on ocular surface with the anticipated forthcoming use of topical probiotics.
Association of birth parameters with refractive status in a sample of caucasian children aged 4-17 years
(Hindawi, 2015-01-01) Akova Budak, Berna; Kıvanç, Sertaç Argün; Olcaysu, Osman Okan; AKOVA, BERNA; KIVANÇ, SERTAÇ ARGUN; Uludağ Üniversitesi/Tıp Fakültesi/Göz Hastalıkları Anabilim Dalı; 0000-0003-0995-5260; ABE-3033-2020; AAH-6518-2021
Purpose. To investigate the association of birth parameters with refractive status in different age groups of Caucasian children. Materials and Methods. This cross-sectional study included 564 eyes of 282 children aged 4 to 17 years. All children underwent complete ophthalmologic examination. The children were divided into three groups according to their refractive status (emmetropia, myopia, and hyperopia), ages (4-7, 8-9, 10-12, and 13-17), and appropriateness for gestational age, respectively. Results. The mean age of the children was 9.2 +/- 2.8 (age range 4-17 years). The mean spheric equivalent was +0.3 +/- 1.7 (range: (-10.0)(+10.0) diopters). The mean birth weight and gestational age were 2681.1 +/- 930.8 grams (750-5000 grams) and 37.2 +/- 3.7 weeks (25-42 weeks). According to multinominal logistic regression analysis, children with myopia were more likely to have higher birth weights than emmetropic children (OR: 1.0, 95% CI: 1.000-1.001, and P = 0.028). The hypermetropes were found to be significantly small for gestational age between 13 and 17 years of age. Conclusion. Birth weight and appropriateness for gestational age as birth parameters may have an impact on development of all types of refractive errors. The hypermetropic children tended to be small for gestational age.
Comparison of two different evaluation methods for biofilm formation of S. epidermidis isolated from ocular surface
(Assoc Research Vision Ophthalmology Inc, 2015-06-01) Budak, Berna Akova; Kıvanç, Sertaç Argün; Yıldız, Meral; Kıvanç, Merih; Güllülü, Gülay; Budak, Berna Akova; KIVANÇ, SERTAÇ ARGUN; YILDIZ, MERAL; Uludağ Üniversitesi; 0000-0003-0995-5260; AAH-1885-2021; AAH-6518-2021; ABE-3033-2020
Demographic characteristics and clinical outcome of work-related open globe injuries in the most industrialised region of Turkey
(Turkish Ophthalmological Soc, 2017-01-01) Çevik, Mediha Tok; KIVANÇ, SERTAÇ ARGUN; Kıvanç, Sertaç Argün; Budak, Berna Akova; AKOVA, BERNA; Skrijelj, Emina; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Oftalmoloji Anabilim Dalı.; 0000-0003-0995-5260; AAH-6518-2021; ABE-3033-2020
Objectives: To evaluate demographic characteristics and clinical outcomes of work-related open globe injuries in the most industrialized region of Turkey.Materials and Methods: The demographic and medical records of patients with work-related open globe injuries who presented to the ophthalmology or emergency departments with an official occupational accident report were retrospectively reviewed. Visual acuity categories were defined according to the World Health Organization. The injury types and zones of the open globes were classified according to Birmingham Eye Trauma Terminology System.Results: Among 479 patients with work-related eye injuries in 5 years, there were 102 eyes of 101 patients with open globe injuries (21%). The mean age of the patients was 34.5 +/- 8.9 years with a mean follow-up of 12.5 +/- 12.6 months. The injuries peaked in June in the hour between 12: 00 and 13: 00. Eighty-six percent presented to emergency services within 12 hours after the injury. Twenty-two percent of the patients had been wearing protective eyewear at the time of injury. The open globe injuries were penetrating in 51%, intraocular foreign body in 40%, rupture in 7% and perforation in 2% of the eyes. The most frequent finding was traumatic cataract. Final visual acuity of 33.3% of patients was below 3/60. Seventy-eight percent of patients that had visual acuity worse than 6/18 at presentation had visual acuity of 6/18 or better at final visit. Sixty-three percent of eyes which had injuries involving all 3 zones resulted in phthisis bulbi, enucleation or evisceration.Conclusion: Work-related open globe injuries may have severe consequences such as visual impairment and blindness among the young male working population in industrialized areas. Nearly half of the occupational open globe injuries resulted in visual impairment and blindness.
Demographics of the open-globe injuries in pediatric age group in northwest Turkey
(Galenos Yayincilik, 2016-12-01) KIVANÇ, SERTAÇ ARGUN; Kıvanç, Sertaç Argün; Budak, Berna Akova; Özmen, Ahmet Tuncer; ÖZMEN, AHMET TUNCER; Yıldız, Meral; YILDIZ, MERAL; Çevik, Mediha Tok; Hamidi, Nagihan Amuk; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Oftalmoloji Anabilim Dalı.; 0000-0003-0995-5260; AAH-1885-2021; AAH-6518-2021; ABE-3033-2020
Introduction: Our aim was to investigate the pattern of open globe injury and assess the effect of age on different parameters of open globe injury in pediatric patients.Materials and Methods: The medical records of the patients under 18 years old who had open-globe injury were retrospectively reviewed. Age, gender, the cause of the trauma, presence of intraocular foreign body and the extent of the ocular damage were recorded.Results: The study was comprised of 79 patients (23 female, 56 male) with a mean age of 7.7+/-4.4 (range: 2-18 years). Thirty-nine patients were injured with a blunt tip object, 27 with a sharp object and one has rupture with trauma. Thirty-five injuries occurred outside and 45 at home. The season in which most of the injuries took place was summer (32%). Seventy-five percent of the injuries were noted in 11 years and under. The mean age subject to blunt tip object injury, organic body injury and outdoor injury was significantly higher compared to that of with sharp object, inorganic body injury and indoor injury.Conclusions: The open globe-injury rates in Northwest Turkey is found to be higher in smaller age groups. Making the parents aware of the danger and consequences of the trauma and avoiding the easily accessible sharp objects may decrease the trauma rates.
Diabetic macular edema and ocular medical treatment options
(Derman Medical Publ, 2015-04-01) Olcaysu, Osman Okan; Kıvanç, Sertaç Argün; KIVANÇ, SERTAÇ ARGUN; Budak, Berna Akova; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Göz Hastalıkları Anabilim Dalı.; 0000-0003-0995-5260; AAH-6518-2021; ABE-3033-2020
Diabetes mellitus (DM) is one of the most common endocrine diseases which causes serious morbidity. Damage occurs in many end organs in diabetic patients. Eyes are also among the end organs and DM causes morbidity related to diabetic retinopathy. It is estimated that 10 % of the diabetic patients have visual impairment and 2 % have blindness. Diabetic macular edema is the main cause for visual impairment in patients with diabetic retinopathy. Treatment of the DME gains importance with new treatment modalities in last decade. Ocular medical treatment modalities were discussed with the review of recent literature.
Does corneal hysteresis correlate with endothelial cell density?
(Int Scientific Literature, 2015-04-29) Akova-Budak, Berna; Kıvanç, Sertaç Argün; AKOVA, BERNA; KIVANÇ, SERTAÇ ARGUN; Uludağ Üniversitesi/Tıp Fakültesi/Göz Hastalıkları Anabilim Dalı.; 0000-0003-0995-5260; AAH-6518-2021; ABE-3033-2020
Background: Our aim was to determine if there is a correlation between corneal biomechanical properties, endothelial cell count, and corneal pachymetry in healthy corneas.Material/Methods: Ninety-two eyes of all subjects underwent complete ocular examination, including intraocular pressure measurement by Goldmann applanation tonometer, objective refraction, and slit-lamp biomicroscopy. Topographic measurements and corneal pachymetry were performed using a Scheimpflug-based (Pentacam, Oculus, Germany) corneal topographer. Corneal hysteresis (CH) and corneal resistance factor (CRF) were measured with an Ocular Response Analyzer (ORA, Reichert Ophthalmic Instruments, Buffalo, NY). Endothelial cell count measurement was done using a specular microscope (CellChek, Konan, USA).Results: Right eye values of the subjects were taken for the study. The mean CH was 11.5 +/- 1.7 mmHg and the mean CRF was 11.2 +/- 1.4 mmHg. Mean intraocular pressure was 15.3 +/- 2.3 mmHg. The mean endothelial cell count was 2754 +/- 205 cells/mm(2). No correlation was found between biomechanical properties of cornea and endothelial cell count. There was a significant positive correlation between CH, CRF, and corneal thickness (p<0.001; r=0.79).Conclusions: The corneal biomechanical properties significantly correlated with corneal thickness. We found no correlation between CH and CRF with the endothelial cell density in normal subjects.
Does smartphone exposure time and smartphone sar value affect ocular surface and refractive error of the dominant eye?
(Assoc Research Vision Ophthalmology Inc, 2018-07-01) Kıvanç, Sertaç Argün; Akova-Budak, Berna; Ulusoy, Mahmut Ouz; Karatepe, Arzu Seyhan; Olcaysu, Osman Okan; KIVANÇ, SERTAÇ ARGUN; AKOVA, BERNA; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Cerrahi Tıp Bilimleri/Göz Hastalıkları; 0000-0003-0995-5260; AAH-6518-2021; ABE-3033-2020
Evaluation of iridocorneal angle, choroidal thickness, and retinal nerve fiber layer thickness in children with a history of retinopathy of prematurity
(Lippincott Williams & Wilkins, 2020-02-01) Ulusoy, Mahmut Oguz; Kal, Ali; Kivanc, Sertac Argun; KIVANÇ, SERTAÇ ARGUN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Oftalmoloji Anabilim Dalı.; AAH-6518-2021
Purpose: Retinopathy of prematurity (ROP) is proliferative retinopathy affecting premature infants associated with abnormal maturation of the retinal vasculature. We sought to evaluate iridocorneal angle, choroidal thickness, and retinal nerve fiber layer thickness (RNFLT) of the children that have a history of ROP using spectral-domain optical coherence tomography. Patients and Methods: Fifty eyes of 28 children with a history of ROP and 46 eyes of 23 healthy school-aged children were included in this study. RNFLT, choroidal thickness, and iridocorneal angle parameters [trabecular iris angle, angle opening distance (AOD500), and trabecular iris space area (TISA500) 500 mu m from the scleral spur] were evaluated using spectral-domain optical coherence tomography. Student t test was used to compare the mean of the parameters. Correlations between the variables were investigated based on the Pearson or Spearman correlation coefficient. Results: Subfoveal (ROP: 253.98 +/- 42.5; control: 286.2 +/- 71.9; P=0.045), 500 mu m (ROP: 242.04 +/- 41.8; control: 276.7 +/- 45.3; P=0.003), 1000 mu m (ROP: 237 +/- 39.7; control: 270.15 +/- 55.93; P=0.007), and 1500 mu m (ROP: 224.16 +/- 37.5; control: 259.75 +/- 55.2; P=0.003) temporal choroidal thicknesses were significantly thinner in ROP history children. None of the RNFLT parameters and ganglion cell complex thickness were different between groups. Iridocorneal angle parameters were significantly lower in children with ROP history. (trabecular iris angle: ROP=31.35 +/- 3.9 degrees, control=35.4 +/- 4.5 degrees, P<0.001; TISA500: ROP=0.167 +/- 0.05 mm(2), control=0.21 +/- 0.05 mm(2), P=0.003; AOD500: ROP=480.96 +/- 160.4 mu m, control=542.95 +/- 161.2 mu m, P=0.035). Conclusions: ROP is associated with differences in the iridocorneal angle. Possible iridocorneal angle pathology should be a consideration in children with a history of ROP.
Evaluation of occupational closed globe eye
(Derman Medical Publ, 2015-06-01) Budak, Berna Akova; Kıvanç, Sertaç Argün; KIVANÇ, SERTAÇ ARGUN; Başkaya, Kevser; Baykara, Mehmet; BAYKARA, MEHMET; Yücel, Ahmet Ali; YÜCEL, AHMET ALİ; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Göz Hastalıkları Anabilim Dalı.; 0000-0003-0995-5260; 0000-0002-5555-1649; ABE-3033-2020; AAH-6518-2021; ABI-7051-2020
Aim: To evaluate closed glob injuries related to occupational accidents of patients who had official occupational accident records. Material and Method: The medical records of patients with ocular injuries who referred to Department of Ophthalmology or emergency of Uludag University, School of Medicine between January 2010 and December 2013 with official occupational accident report were retrospectively reviewed. The patients with closed globe injuries following trauma were included. Age, sex, the injured eye, the cause of the trauma, whether the precautions were taken or not by the patient, the damage due to trauma were recorded. Results: According to the official records, 108 patients referred to our clinic with closed globe injury related to occupational accident. One hundred twenty eyes of 108 patients (2 females, 106 males) were evaluated. The mean age of the patients was 33 +/- 8.6 years. The most frequent cause of injury was foreign bodies on the ocular surface followed by blunt trauma. The mean age of the patients injured with foreign bodies was found to be significantly lower than the patients injured with blunt objects (p=0.039). Thirteen patients reported that they had used preventive equipment. Discussion: It is of utmost importance that the awareness of the workers should be raised and they should be educated about the use of preventive equipment to prevent the occupational eye injuries. The education of particularly the younger patients about the occupational injuries when they begin to work may decrease the rate of occupational accident related eye injuries.
Is ciliary muscle affected in migraine patients with aura and without aura?
(Int Scientific Information, 2015-01-02) Kıvanç, Sertaç Argün; Ulusoy, Mahmut Oğuz; Akova-Budak, Berna; Olcaysu, Osman Okan; Özcan, Muhammed Emin; KIVANÇ, SERTAÇ ARGUN; AKOVA, BERNA; Uludağ Üniversitesi/Tıp Fakültesi/Göz Hastalıkları Anabilim Dalı.; 0000-0003-0995-5260; AAH-6518-2021; ABE-3033-2020
Background: The aim of this study was to compare spherical equivalents (SE) and spherical equivalents with cycloplegic (CSE) values of migraine patients with and without visual aura, with those patients without migraine complaints.Material/Methods: We included patients over the age of 18 years, who had 20/20 uncorrected vision, and who did not have ocular pathology in their examination. The patients were divided into 3 groups: Group 1: 86 eyes of 43 migraine patients without aura, Group 2: 38 eyes of 19 migraine patients with aura, and Group 3: 60 eyes of 30 patients without migraine. Spherical equivalents and spherical equivalents with cycloplegic from the auto-refractometrical values of the patients were compared.Results: The mean age of the migraine and control patients was 34.2 +/- 8.3 and 33.6 +/- 10.8 years, respectively. Forty-three (69%) of 62 migraine patients had migraine without aura and 19 (31%) had migraine with aura. The right and left eyes of the patients were evaluated together and a significant correlation was found between the groups. To evaluate the impact of cycloplegia in patients, SE and CSE values were obtained and differences between these values were evaluated. It was found that the change in Group 2 patients was significantly lower than the change in Group 3 patients (p=0.024).Conclusions: We found that the cycloplegic spherical equivalents values of our patients with aura were lower than control patients. We need further studies to reveal whether migraine with aura is the trigger or the result of those attacks.
Palpebral tarsal solitary neurofibroma
(Turkish Ophthalmological Soc, 2019-08-01) Yazıcı, Bülent; Kıvanç, Sertaç Argün; KIVANÇ, SERTAÇ ARGUN; Yayla, Uğur; Adım, Saduman Balaban; BALABAN ADIM, ŞADUMAN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Oftalmoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı.; 0000-0001-8889-1933; AAA-5384-2020; AAH-6518-2021; JCE-2538-2023
Solitary neurofibroma is a rare, benign tumor of the peripheral nerve sheath, and is often associated with neurofibromatosis type 1. Herein, a case of palpebral tarsal solitary neurofibroma in a patient without neurofibromatosis is presented, with a review of the literature. A 68-year-old man presented with a subcutaneous mass in the right upper eyelid of 6 months' duration. Eversion of the eyelid revealed a round, reddish mass on the lateral part of the tarsal plate which measured 12x8 mm in size. The lesion was excised with its tarsal base, diagnosed histologically, and did not recur during a follow-up of 34 months. Isolated, solitary neurofibroma of the eyelid has been reported in a total of 7 cases, including the case presented herein. The tumors arose from the eyelid margin in 4 cases, from the tarsal plate in 2 cases, and from the supratarsal conjunctiva in 1 case. The tumor did not recur after surgical excision in 5 cases for which follow-up data were available.
Post-thyroidectomy iatrogenic horner's syndrome with heterochromia
(Elsevier, 2016-03-01) Ulusoy, Mahmut Oğuz; Kıvanç, Sertaç Argun; Atakan, Mehmet; Mayalı, Hüseyin; KIVANÇ, SERTAÇ ARGUN; Uludağ Üniversitesi/Tıp Fakültesi/Göz Hastalıkları Anabilim Dalı; AAH-6518-2021
Purpose: To present a case of iatrogenic Horner's syndrome seen together with the heterochromia in the post-thyroidectomy period.Methods: A 23-year-old female patient was admitted to our clinic with complaints of low vision in the eye and difference in eye color that developed over the past two years. In the left eye, myosis and minimal ptosis (similar to 1 mm) was detected, and the color of the iris was lighter than the right eye.Results: The pre-diagnosis of left iatrogenic Horner's syndrome was finalized after 0.5% topical apraclonidine test.Conclusion: Heterochromia can be observed in iatrogenic Horner's syndrome. Copyright (c) 2016, Iranian Society of Ophthalmology. Production and hosting by Elsevier B.V.
Spectral domain optical coherence tomography findings in Turkish sickle-cell disease and beta thalassemia major patients
(Wolters Kluwer Medknow Publications, 2019-09-01) Ulusoy, Mahmut Oğuz; Türk, Hakan; Kıvanç, Sertaç Argun; KIVANÇ, SERTAÇ ARGUN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Göz Hastalıkları Anabilim Dalı; AAH-6518-2021
Purpose: To assess probable structural changes using spectral domain optical coherence tomography (SD-OCT) on sickle-cell disease (SCD) and beta thalassemia major (B-TM) patients, without any retinal abnormalities.Methods: This cross-sectional study included 32 B-TM, 34 SCD patients, and 44 healthy controls. One of the eyes of all participants was evaluated for SD-OCT and choroidal thickness, retinal nerve fiber layer (RNFL) thickness, central macular thickness (CMT), ganglion cell complex (GCC).Results: Age, gender, and intraocular pressure (IOP) were not statistically different between the three groups. Hemoglobin (Hgb), hematocrite (Htc), and ferritin levels were not statistically different between the SCD and B-TM groups. Choroidal thickness at the subfoveal region was statistically higher in the control group (353.79 +/- 71.93) than in the B-TM (317.41 +/- 53.44) and SCD (283.21 +/- 63.27) groups. In addition, it was statistically higher in the B-TM group than the SCD group (P = 0.05). CMT did not differ among the three groups, average RNFL was only significantly thinner in SCD than in controls, and GCC thickness was significantly thinner in SCD than in controls and B-TM.Conclusion: In both diseases, we can show early structural changes even if proliferative or non-proliferative retinopathy or other ocular manifestations were not developed yet. Copyright (C) 2019, Iranian Society of Ophthalmology. Production and hosting by Elsevier B.V.
The biofilm formation properties of the S. epidermidis isolates obtained from conjunctiva and multi-drug resistance
(Kuwait Medical Assoc, 2020-06-01) Kılıç, Volkan; Kıvanç, Merih; Kıvanç, Sertaç Argün; KIVANÇ, SERTAÇ ARGUN; Akova, Berna; AKOVA, BERNA; Bursa Uludağ Üniversitesi/Tıp Fakültesi; 0000-0003-0995-5260; AAH-6518-2021; ABE-3033-2020
Objective: Biofilm was shown on ocular abiotic surfaces such as contact lenses, intra-ocular lenses, glaucoma shunts and corneal sutures. The aim of this study is to compare success of Microtiter plate (MTP) and Congo Red Agar (CRA) methods in showing biofilm formation of S.epidermidis isolates that were obtained from conjunctiva regarding presence of icaA gene locus and multi-drug resistance.Design: InterventionalSetting: Department of Biology, Faculty of Science, Eskisehir Technical UniversitySubjects: S. epidermidis isolates obtained from conjunctivas of healthy patientsInterventions: MTP and CRA methods and antibiotic susceptibility tests were done and scanning electron microscopy was performed. Main outcome measures: Biofilm forming capacity, multidrug resistance states and bacterial adhesion of S. epidermidisResults: Nine isolates were found as strong biofilm producers with MTP and CRA methods. There were six icaA negative isolates, one of which was not a biofilm producer. From the icaA and icaD positive isolates, 13 were biofilm negative with MTP method and seven were biofilm negative with CRA method. Eighteen isolates were determined as resistant to three or more antibiotics. Ten of 18 isolates were strong biofilm producer with MTP and eight of 18 isolates were strong biofilm producer with CRA. Biofilm producer isolates were found to be resistant to antibiotics.Conclusion: It is noted that biofilm formation capacity of S. epidermidis isolates that were obtained from ocular surface were determined and multidrug resistance were estimated more successfully with MTP method. However, there is no method which can successfully detect biofilm formation capacity with 100% accuracy.
The effect of the linezolid and the vancomycine on biofilm production that formed on two different acrylic hydrophobic intraocular lenses
(Assoc Research Vision Ophthalmology Inc, 2015-06-01) Kıvanc, Sertaç Argün; Budak, Berna Akova; Yıldız, Meral; Kıvanç, Merih; KIVANÇ, SERTAÇ ARGUN; Budak, Berna Akova; YILDIZ, MERAL; Uludağ Üniversitesi; 0000-0003-0995-5260; AAH-1885-2021; GNH-1796-2022; ABE-3033-2020; AAH-6518-2021
The newborn conjunctival flora at the post delivery 24 hours
(Wolters Kluwer Medknow Publications, 2018-12-01) Kara, Mustafa; Kıvanç, Sertaç Argun; Olcaysu, Osman Okan; Budak, Berna Akova; Özmen, Ahmet Tuncer; Kıvanç, Merih; Öz, Hatice Tuna Hormet; KIVANÇ, SERTAÇ ARGUN; Budak, Berna Akova; ÖZMEN, AHMET TUNCER; Uludağ Üniversitesi/Tıp Fakültesi/Göz Hastalıkları Anabilim Dalı.; 0000-0003-0995-5260 ; AAH-6518-2021; ABE-3033-2020; AAH-6550-2021
Purpose: The aim of this study was to investigate the aerobic conjunctival flora of neonates and the effects of delivery type on conjunctival flora development in neonates who were born with normal spontaneous vaginal delivery (SVD) or elective caesarean section (C/S) and who were not given prophylactic antibiotic eye drops after birth.Methods: This cross-sectional study included 95 healthy newborns. One day after the delivery, conjunctival samples were taken from newborns who were born with normal SVD or elective C/S, and not given prophylactic antibiotic eye drops after birth. Newborns with conjunctival hyperemia and discharge were excluded from study. Samples were plated in blood agar, EMB, and chocolate agar. These cultures were incubated at 37 degrees C for 24-48 h. Antibiotic sensitivity was evaluated using Kirby-Bauer disc diffusion method.Results: Staphylococcus aureus (S.aureus) growth was observed in 7 (70%) and coagulase negative staphylococcus (CNS) growth in 2 (20%) out of 10 eyes with bacterial growth in 9 culture positive newborns born with C/S. Two S. aureus strains were resistant to methicillin. On the other hand, CNS growth was observed in the conjunctival cultures of 17 out of 19 eyes with bacterial growth in 16 culture positive newborns born with SVD. In 2 eyes with CNS growth, there was also S. aureus growth. The positive cultures for S. aureus were significantly higher in the conjunctival cultures of neonates born with C/S compared to neonates born with SVD, where CNS growth was significantly lower (P = 0.002). All isolates were susceptible to vancomycin, teicoplanin, and gatifloxacin. Two isolates were resistant to methicillin.Conclusions: In deliveries with C/S, the newborn does not contact the vagina. This may result in changes of bacterial characteristic of the flora. Culture positivity for S. aureus was higher in C/S compared to SVD, which may be important in case neonatal conjunctivitis develops. Copyright (C) 2018, Iranian Society of Ophthalmology. Production and hosting by Elsevier B.V.