Browsing by Author "Kahveci, Nevzat"
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Item An anatomical and pathological evaluation of middle cerebral artery occlusion in rats(Taylor & Francis, 2000) Kahveci, Nevzat; Alkan, Tülin; Korfalı, Ender; Özlük, Kasım; Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Beyin Cerrahisi Anabilim Dalı.; 0000-0003-0841-8201; AAG-7070-2021; AAH-1792-2021Adult male Sprague-Dawley rats (n = 87) weighing 350-400g were used for studying the anatomy of the horizontal segment of middle cerebral artery and infarct area after occlusion of the artery. In the experimental group (n = 27) middle cerebral artery was coagulated 3-4 mm length from the origin of the lateral striate arteries to the inferior cerebral vein and divided. Control rats (n = 20) had all the surgical procedures except occlusion. Another group of rats (n = 40) were used to determine the anatomical variations of middle cerebral artery after intracarotid carbon black injection. Five major patterns of middle cerebral artery were observed and two of them were major and constituted 92.5% of rats. Twenty-four hours after middle cerebral artery occlusion, all animals were neurologically evaluated. On the third day after occlusion the brains were stained with 2% 2,3,5-triphenyltetrozolium chloride. The area of infarction was assessed by computerized analysis method. In our study after determining the Variations of the middle cerebral artery and its branches in our strain of rats, we were able to achieve 92.5% grade III and IV infarcted area.Publication Can heat shock protein 32 be used for the early diagnosis of acute mesenteric ischemia?(Türk Cerrahi Derneği, 2016-03-01) Berhuni, Sait; Öztürk, Ersin; Oral, Arzu Yılmaztepe; Sarkut, Pınar; Kahveci, Nevzat; Yılmazlar, Tuncay; Özlük, Kasım; Yerci, Ömer; Berhuni, Sait; Öztürk, Ersin; YILMAZTEPE ORAL, ARZU; Sarkut, Pınar; KAHVECİ, NEVZAT; YILMAZLAR, AHMET TUNCAY; Özlük, Kasım; YERCİ, ÖMER; Uludağ Üniversitesi/Tıp Fakültesi/Genel Cerrahi Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı.; 0000-0002-8962-9758; 0000-0003-0841-8201; AAG-7070-2021; A-5841-2017; CEH-4566-2022; JGW-0566-2023; HKB-5363-2023; CKK-3621-2022; JJX-0104-2023; EIS-5114-2022Objective: Acute mesenteric ischemia is a challenging and fatal disease. The aim of this study was to detect the heat shock protein 32 (HSP32) response in intestinal tissue and systemic blood to intestinal ischemia and ischemia/reperfusion to define a tool for the early diagnosis of acute mesenteric ischemia.Material and Methods: Thirty female Wistar albino rats were equally divided into 3 groups. Group 1 rats underwent simple laparotomy and closure (control). In Group 2 rats, 1-hour intestinal ischemia followed by 5-hour reperfusion was performed, and Group 3 rats were subjected to 6-hour intestinal ischemia. The experiment was repeated with a 24-hour waiting period. At the end of the waiting period, blood was withdrawn from the tail veins of the rats and the rats were sacrificed via cardiac puncture. Re-laparotomy was subsequently performed and intestinal tissue and luminal samples were obtained for biochemical and pathological investigations. The HSP32 levels of intestinal tissues, luminal contents and blood levels were compared among the groups.Results: At the end of the 24-hour waiting period, the median tissue HSP32 levels were 0.43 (0-6.6) ng/mL for Group 1, 9.51 (2.5-49.9) ng/mL for Group 2 and 43.13 (6.3-121.3) ng/mL for Group 3 (p= 0.001). The median blood HSP32 levels were 0.11 (0.1-1.4) ng/mL for Group 1, 0.42 (0.1-0.7) ng/mL for Group 2, and 0.25 (0.1-1.2) ng/mL for Group 3 (p= 0.047). The HSP levels in the luminal contents were undetectable.Conclusion: Both ischemia and ischemia/reperfusion significantly raised intestinal tissue HSP32 levels in comparison with the control group. However, this change was not reflected in the circulating blood or luminal contents.Item CDP-kolinin uyku yoksunluğu oluşturulan sıçanlarda öğrenme ve bellek parametreleri üzerine etkisi(Bursa Uludağ Üniversitesi, 2018-09-28) Çakır, Ayşen; Kahveci, Nevzat; Süyen, Güldal; Bursa Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü/Fizyoloji Anabilim Dalı.Bu çalışmada, hızlı göz hareketleri (REM) uyku yoksunluğunun öğrenme ve bellek üzerine bilinen olumsuz etkilerine karşı farklı dozlarda sitidin 5-difosfokolin (CDP-kolin) uygulamasının etkileri incelenmiştir. Wistar albino cinsi erkek sıçanlar (n=72, 200-300 g, 8-12 haftalık) randomize olarak 12 gruba ayrılarak uygun kafeslerde takip edilmiştir. "Flower pot" tekniği kullanılarak hayvanlar 4 gün boyunca 6.5 cm çapında bir platform üzerinde bırakılarak uyku yoksunluğu, 13 cm çapında platform üzerinde bırakılarak ortam kontrol grupları oluşturulmuştur. Morris su tankı testi ile dört gün boyunca günde iki kez eğitim fazı, 5. gün probe fazı gerçekleştirilmiştir. Morris su tankı testlerinden 30 dakika önce sıçanlara intraperitoneal olarak serum fizyolojik veya 100 µmol/kg, 300 µmol/kg, 600 µmol/kg dozunda CDP-kolin enjeksiyonu yapılmıştır. Davranış deneylerinin ardından hayvanlar dekapite edilip hipokampus bölgelerinden elde edilen homojenatlarda; Western-blot yöntemiyle fosforile siklik adenozin monofosfat yanıt elemanı bağımlı protein (pCREB), total kalsiyum kalmodulin bağımlı kinaz II (tCaMKII) ve β-Tubulin proteinlerinin analizleri yapılmıştır. Çalışmamızda REM uyku yoksunluğunun öğrenme parametreleri üzerine etkisi olmadığı, bellek parametrelerini ise olumsuz yönde etkileyebileceği gözlemlenmiştir. CDP-kolin tedavisinin bozulan bellek üzerine olumlu yönde etki edebileceği saptanmıştır. Ayrıca uyku yoksunluğu oluşturmak için kullanılan model nedeniyle hayvanların maruz kaldığı stresin öğrenme ve bellek parametrelerini etkileyebileceği ve CDP-kolinin stres üzerine olumlu etkilerinin olabileceği gözlenmiştir. Western blot analizleri sonucunda ise total CaMKII oranı değişmezken, pCREB oranı uyku yoksunluğuna bağlı anlamlı olarak azalmıştır.Item Çocukluk çağı kafa travmalarında kan glukoz düzeyi ve vücut sıcaklığının prognoza etkisi(Bursa Uludağ Üniversitesi, 2022-06-14) Çakır, Ayşen; Durak, Vahide Aslıhan; Taşkapılıoğlu, M. Özgün; Özkaya, Güven; Kahveci, Nevzat; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Acil Tıp Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Beyin ve Sinir Cerrahisi Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Biyoistatistik Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı.; 0000-0001-7729-7373; 0000-0003-0836-7862; 0000-0001-5472-9065; 0000-0003-0297-846X; 0000-0003-0841-8201Pediatrik kafa travması çocukluk çağının önemli mortalite ve morbidite sebepleri arasındadır. Acil servise başvuru anındaki parametrelere göre prognozun önceden bilinmesi tedavi ve yakın takip için uyarıcı olabilecektir. Bu çalışmada başvuru anındaki kan glukoz değerinin ve vücut sıcaklığının prognoz üzerine etkisinin Modifiye Rankin Skoru ile değerlendirilmesi planlanmıştır. Çalışmada Bursa Uludağ Üniversitesi Tıp Fakültesi Acil Servisi’ne başvuran 0-16 yaş aralığındaki 301 olgu incelenmiştir. Başvuru anındaki Glasgow Koma Skoru ile kan glukoz değeri arasında ters yönde korelasyon saptanmıştır. Ayrıca Glasgow Koma Skoru ile Modifiye Rankin Skoru arasında da ters yönde korelasyon gözlenirken, kan glukoz değeri ile Modifiye Rankin Skoru arasında pozitif yönde zayıf korelasyon saptanmıştır. Başvuru anında saptanan hiperterminin prognoz üzerine etkisinin olmadığı gözlenmiştir. Bu sonuçlar başvuru anındaki Glasgow Koma Skorunun yanı sıra kan glukoz değerinin yüksekliğinin prognoz tayininde önemli olabileceğini göstermiştir.Item Decompressive craniectomy at the treatment of cerebral infarct in rats(Monduzzi Editore, 1997) Kahveci, Nevzat; Bekar, Ahmet; Korfalı, Ender; Özlük, Kasım; Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Nöroşirurji Anabilim Dalı.; AAG-7070-2021Effects of decompressive craniectomy after permanent middle cerebral artery occlusion (MCAO) were investigated in rats. In control group (n:13) MCA was couterized via a 3mm subtemporal craniectomy. In decompression group (n:11) after MCAO through 3mm craniectomy additional bone (9x5 mm) was removed dawn to the floor of the middle and temporal fossa and dura left open. 24 h after occlusion the rats were neurologically examined. After decapitation brains were stained with TTC and infarct areas were evaluated with computerized analysing method. Although the mortality in the control group was %15.3 none of the rats in decompressive craniectomy group died. Neurological scoring and infarction size were significantly better in the latter group. The results suggest that decompressive craniectomy reduces mortality, significantly improves neurological outcome and reduces infarction size.Item Depresyon oluşturulan sıçanlarda linopirdin'in etkisi(Uludağ Üniversitesi, 2010) Uzunok, Barış; Kahveci, Nevzat; Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı.Bu çalışmada, sıçanlarda Zorlu Yüzme Testi (ZYT) ile oluşturulan depresyon modelinde Kv7 tipi voltaj kapılı potasyum kanallarının depresyon üzerindeki etkisinin incelenmesi amaçlandı. Bu amaçla sıçanlara yüzme testinin ikinci gününde, testten 15 dakika önce intraserebroventriküler (i.c.v.) olarak salin (4 µl) veya Kv7 tipi voltaj kapılı potasyum kanal blokörü olan Linopirdin (0.1, 1, 10 µg/4 µl) uygulandı. Linopirdin (0.1 µg/4 µl; i.c.v.) kontrol grubuna göre hareketsizliği anlamlı olarak azaltırken (p<0.01), yüzmeyi anlamlı olarak arttırdı (p<0.01). Linopirdin (1 µg/4 µl ve 10 µg/4 µl; i.c.v.) kontrol grubuna göre hareketsizliği anlamlı olarak azaltırken (p<0.001), yüzme ve tırmanma hareketini anlamlı olarak arttırdı (p<0.05).Elde ettiğimiz sonuçlar i.c.v. olarak uygulanan Linopirdin'in anti-depresan benzeri etki gösterdiğini düşündürmektedir. Buna göre, ZYT ile oluşturulan depresyon modelinde Kv7 kanallarının etkisi olduğu sonucuna varıldı.Item Dexamethasone prevents hypoxic-ischemic brain damage in the neonatal rat(Monduzzi Editore, 1997) Alkan, Tülin; Kahveci, Nevzat; Kahveci, Zeynep; Korfalı, Ender; Özlük, Kasım; Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Nöroşirurji Anabilim Dalı.; AAH-1792-2021; AAG-7070-2021In our study the effect of dexamethasone 3 hours before the hypoxic-ischemic brain damage were investigated in neonatal rats. 7 day old rats were undergone right CCA ligation followed by 2 hours hypoxic exposure. 7 day after the insult the brains were sectioned coronally +2, -2 mm from bregma and immersed in TTC. In vehicle treated group there was a marked infarction throughout cortex and striatum, disintegration of the structures of the right hemisphere and liquefication. In the Ist coronal slice 18.33% and in the IInd 32.76% of infarct area was detected. H&E and Nissl stainings also showed extensive neuronal degeneration. Dexamethasone group had no gross infarction and histopathological examination. showed only mild degree of ischemic changes.Item Early stage alterations in CA1 extracellular region proteins indicate dysregulation of IL6 and iron homeostasis in the 5XFAD Alzheimer's disease mouse model(IOS Press, 2018) Gürel, Büşra; Keleştemur, Seda; Ozansoy, Mehmet; Ulus, İsmail Hakkı; Başar, Merve Karayel; Şahin, Betül; Tuzuner, Mete Bora; Baykal, Ahmet Tarık; Cansev, Mehmet; Sevinç, Cansu; Öcalan, Büşra; Çakır, Ayşen; Aydın, Sami; Kahveci, Nevzat; Taşkapılıoğlu, Özlem; Uludağ Üniversitesi/Tıp Fakültesi/Eczacılık Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Nöroloji Anabilim Dalı.; 0000-0002-3405-3640; 0000-0001-7729-7373; 0000-0003-0841-8201; 0000-0003-4436-3797; M-9071-2019; N-9927-2019; A-6819-2018; AAG-7070-2021; X-4479-2018; 8872816100; 56473593500; 57191911801; 57191915856; 7005387015; 6602597846; 23037226400In recent years, an increasing number of research papers revealed that the compositional and volumetric alterations in the extracellular matrix are the consequences of aging and may be related to Alzheimer's disease (AD). In this study, we aimed to demonstrate the alterations in hippocampal extracellular fluid proteins in vivo using the 5XFAD mouse model. Samples were obtained from hippocampi of 5XFAD mice (n = 6) and their non-transgenic littermates by intracerebral push-pull perfusion technique at 3 months of age, representing the pre-pathological stage of the AD. Proteins in the hippocampal perfusates were analyzed by Ultra Performance Liquid Chromatography-Electrospray Ionization Quadrupole Time-of-Flight Mass Spectrometry (UPLC-ESI-qTOF-MS/MS). 178 proteins were identified and 19 proteins of them were found to be statistically significantly altered (p <= 0.05, fold change >= 40%, unique peptide count >= 3) in the hippocampal CA1 extracellular fluid of the 5XFAD mouse model. Ingenuity pathway analysis of the protein expression results identified IL6 as an upstream regulator. The upregulation of IL6 was validated by immunohistochemical staining of the hippocampus and cortex of the 5XFAD mice prior to A beta plaque formation. Furthermore, the iron level in the hippocampus was measured by inductively coupled plasma-mass spectrometry as IL6 is mentioned in several studies to take part in iron homeostasis and inflammation and found to be increased in 5XFAD mice hippocampus. Alterations in extracellular matrix proteins in addition to increasing amount of hippocampal IL6 and iron in the early stages of AD may reveal inflammation-mediated iron dyshomeostasis in the early stages of neurodegeneration.Item The effect of CDP choline on learning and memory in sleep deprivation(Wiley, 2017-09) Süyen, Güldal; Çakır, Ayşe; Öçalan, Büşra; Sevinç, Cansu; Cansev, Mehmet; Kahveci, Nevzat; Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı.; 0000-0001-7729-7373; 0000-0002-3405-3640; A-6819-2018; N-9927-2019; M-9071-2019; AAG-7070-2021Item The effect of CDP-choline on learning and memory parameters in sleep deprivated rats(Wiley, 2016-09) Süyen, Güldal; Çakır, Ayşen; Öcalan, Büşra; Sevinç, Cansu; Cansev, Mehmet; Kahveci, Nevzat; Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Fizyoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Farmakoloji Anabilim Dalı.; 0000-0002-6097-5585; 0000-0001-7729-7373; 0000-0003-0863-1547; 0000-0002-3405-3640; AAG-7070-2021; AAA-4754-2022; A-6819-2018; C-5730-2015; N-9927-2019; M-9071-2019Publication Effects of cdp-choline administration on learning and memory in rem sleep-deprived rats(Pergamon-elsevier Science Ltd, 2020-01-01) Çakır, Aysen; Öcalan, Busra; Suyen, Guldal Güleç; Kahveci, Nevzat; Koç, Cansu; KOÇ, CANSU; Cansev, Mehmet; CANSEV, MEHMET; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji Anabilim Dalı.; 0000-0002-6097-5585; 0000-0003-2918-5064; AAA-4754-2022; AAL-1786-2020; M-9071-2019Cytidine 5-diphosphocholine (CDP-choline) administration has been shown to improve learning and memory deficits in different models of brain disorders. In this study, effects of CDP-choline on the well known negative effects of Rapid Eye Movements (REM) sleep deprivation on learning and memory were investigated. Sleep deprivation was induced by placing adult male Wistar albino rats on 6.5 cm diameter platforms individually for 96 h according to flower pot method. Learning and memory performances were evaluated using Morris Water Maze (MWM) test during the same period of time. Saline or CDP-choline (100 mu mol/kg, 300 mu mol/kg or 600 mu mol/kg) was administered intraperitoneally 30 min prior to the onset of MWM experiments. On completion of behavioral tests, rats were decapitated and hippocampi were assayed for total and phosphorylated Ca2+/calmodulin-dependent protein kinase II (tCaMKII and pCaMKII, respectively) and total antioxidant capacity. We observed that while REM sleep deprivation had no effect on learning, it diminished the memory function, which was associated with decreased levels of pCaMKII and total antioxidant capacity in the hippocampus. CDP-choline treatment blocked the impairment in memory function of sleep-deprived rats and, increased pCaMKII levels and total antioxidant capacity. These data suggest that CDP-choline reduces REM sleep deprivation-induced impairment in memory, at least in part, by counteracting the disturbances in biochemical and molecular biological parameters.Item Effects of centrally-injected glucagon-like peptide-1 on pilocarpine-induced seizures, anxiety and locomotor and exploratory activity in rat(Churchill Livingstone, 2010-08) Güleç Süyen, Güldal; İşbil Büyükcoşkun, Naciye; Kahveci, Nevzat; Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı.; 0000-0003-0863-1547; 0000-0003-0841-8201; AAH-1692-2021; AAG-7070-2021; C-5730-2015; 6602752303; 55665951400; 6602597846Glucagon-like peptide-1 (7-36)-amide (GLP-1) is a gut peptide, which exerts significant effects on glucose homeostasis. GLP-1 and GLP-1 receptors are also widely distributed in the central nervous system. In the present study, we aimed to investigate the effects of intracerebroventricularly (i.c.v.)-injected GLP-1 on pilocarpine-induced seizures, anxiety and locomotor and exploratory activity in rat. Rats were pretreated with GLP-1 (1-1000 ng/5 mu l: i.c.v.) or saline (5 mu l; i.c.v.) 30 min before seizure induction by pilocarpine (2.4 mg/5 mu l; i.c.v.) and with GLP-1 (1, 10, 100 ng/5 mu l; i.c.v.) or saline (5 mu l; i.c.v.) 30 min before the open field test or the elevated plus maze test. GLP-1 did not produce any protective effect against pilocarpine-induced seizures and did not also produce statistically significant differences in the number of squares visited (measure of locomotor activity) or number of rearings (measure of exploratory behaviour), compared to the saline-treated rats in the open field test. On the other hand, GLP-1 (1 ng and 10 ng; icy.) induced an anxiogenic effect, indicated by a decrease in the time spent in open arms, an increase in the time spent in closed arms, and a decrease in the anxiety scores in the elevated plus maze test. Pretreatment with an arginine vasopressin (AVP) V(1) receptor antagonist (125 ng/5 mu l; i.c.v.) and L-NAME (100 mu g/5 mu l and 200 mu g/5 mu l) significantly abolished the anxiogenic effect of GLP-1 (1 ng/5 mu l; i.c.v.). These results suggest that, centrally-injected GLP-1 produces anxiogenic effects via NO pathway and AVP V(1) receptors, but does not have any effects on pilocarpine-induced seizures or locomotor and exploratory activity in the open field test.Item Effects of centrally-injected glucagon-like peptide-1 on pilocarpine-induced seizures, anxiety and locomotor and exploratory activity in rat(Wiley, 2010-06) Kahveci, Nevzat; Güleç, Güldal Süyen; Büyükcoşkun, Naciye İşbil; Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı.; 0000-0003-0863-1547; AAG-7070-2021; AAH-1692-2021; C-5730-2015; 6602752303; 55665951400; 6602597846Glucagon-like peptide-1 (7-36)-amide (GLP-1) is a gut peptide, which exerts significant effects on glucose homeostasis. GLP-1 and GLP-1 receptors are also widely distributed in the central nervous system. In the present study, we aimed to investigate the effects of intracerebroventricularly (i.c.v.)-injected GLP-1 on pilocarpine-induced seizures, anxiety and locomotor and exploratory activity in rat. Rats were pretreated with GLP-1 (1-1000ng/5μl; i.c.v.) or saline (5μl; i.c.v.) 30min before seizure induction by pilocarpine (2.4mg/5μl; i.c.v.) and with GLP-1 (1, 10, 100ng/5μl; i.c.v.) or saline (5μl; i.c.v.) 30min before the open field test or the elevated plus maze test. GLP-1 did not produce any protective effect against pilocarpine-induced seizures and did not also produce statistically significant differences in the number of squares visited (measure of locomotor activity) or number of rearings (measure of exploratory behaviour), compared to the saline-treated rats in the open field test. On the other hand, GLP-1 (1ng and 10ng; i.c.v.) induced an anxiogenic effect, indicated by a decrease in the time spent in open arms, an increase in the time spent in closed arms, and a decrease in the anxiety scores in the elevated plus maze test. Pretreatment with an arginine vasopressin (AVP) V1 receptor antagonist (125ng/5μl; i.c.v.) and L-NAME (100μg/5μl and 200μg/5μl) significantly abolished the anxiogenic effect of GLP-1 (1ng/5μl; i.c.v.). These results suggest that, centrally-injected GLP-1 produces anxiogenic effects via NO pathway and AVP V1 receptors, but does not have any effects on pilocarpine-induced seizures or locomotor and exploratory activity in the open field test.Item Effects of different resuscitation fluids on tissue blood flow and oxidant injury in experimental rhabdomyolysis(Lippincott Williams & Wilkins, 2005-11) Özgüç, Halil Bülent; Kahveci, Nevzat; Akköse, Şule; Serdar, Zehra; Balci, Veysel; Ocak, Özgür; Uludağ Üniversitesi/Tıp Fakültesi/Cerrahi Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Bölümü.; Uludağ Üniversitesi/Tıp Fakültesi/Acil Tıp Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; 0000-0003-0841-8201; AAG-7070-2021; 6603867989; 6602597846; 6603347542; 57222002284; 6507877217; 9940943800Objective: This study was performed to evaluate the effects of 0.9% saline (SAL), 0.9% saline+sodium bicarbonate+mannitol (SAUBIC/MAN), and hypertonic saline-dextran (HSD) on hemodynamic variables, tissue blood flow, and oxidant injuries in experimental traumatic rhabdomyolysis (TR) in rats subjected allogeneic muscle extract infusion. Design: Prospective, randomized, experimental. Setting: Physiology experiment laboratory. Subjects: Male Sprague-Dawley rats, weighing 250-300 g. Interventions: All groups (n=8 each) underwent femoral artery and vein catheterization. The animals in the TR, SAL, SAUBIC/MAN, and HSD groups received an infusion of 2 mL of autologous muscle extract for 60 mins. After autologous muscle extract infusion, the SAL and HSD groups received 30 mL/kg 0.9% saline for 30 mins or 4 mL/kg HSD for 5 mins, respectively. The SAUBIC/MAN group received 30 mL/kg 0.9% saline for 30 mins plus a bolus of 1 g/kg mannitol and a bolus of 2 mEq/kg sodium bicarbonate diluted in 1 mL of saline. At 2 hrs of autologous muscle extract infusion, erythrocyte flows in liver and kidney were measured by using a laser Doppler flowmeter. Then, blood samples and kidney and liver biopsies were taken to measure levels of glutathione and malondialdehyde. Measurements and Main Results: TR caused decreases in mean arterial pressure, tissue blood flow, and tissue glutathione and an increase in malondialdehyde. Rats in the HSD group had significant metabolic acidosis. SAL resuscitation did not correct tissue blood flow and prevent oxidant injury. HSD increased tissue blood flow, mean arterial pressure, and liver and kidney glutathione and decreased serum, liver, and kidney malondialdehyde. SAUBIC/MAN resuscitation corrected all oxidant damage variables but did not increase tissue blood flow. SAUBIC/MAN preserved serum malondialdehyde and liver glutathione better than the HSD did. Conclusions: HSD prevented oxidant injury and restored tissue blood flow but increased metabolic acidosis that followed autologous muscle extract infusion. SAUBIC/MAN seems to be more effective than HSD in decreasing oxidant injury. Further research on the effects of the solute overload and metabolic acidosis due to HSD resuscitation on renal function in experimental rhabdomyolysis is warranted.Item The effects of drugs on intracranial pressure, cerebral perfusion pressure and histopathological investigation of the infarct areas in focal cerebral ischemia model in rats(Monduzzi Editore, 1997) Kahveci, Nevzat; Alkan, Tülin; Kahveci, Ferda Şöhret; Korfalı, Ender; Özlük, Kasım; Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Anesteziyoloji ve Reanimasyon Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Nöroşirurji Anabilim Dalı.; AAH-1792-2021; AAG-7070-2021The effects of neuroprotective drugs in focal cerebral ischemia induced after MCA occlusion were investigated in adults rats. Group I rats (n:10) received nimodipine (1 mg/kg/min); Group II (n:10) etomidate (75 mg/kg, iv + 0.5 mg/kg/min iv maintenance); Group III (n:10) lidocaine (5 mg/kg iv + 0.4 mg/kg/min iv maintenance); Group IV (n:12) vehicle solution. In all groups rats were observed 300 min after MCAO and MABP, ICP measurements were recorded at 30 min intervals. There was no change in MABP values in Group I and II whilst these values decreased in Group III and IV. ICP increased in all groups. 24 h after occlusion in TTC stained brain sections smaller infarct areas were observed in Group I and II comparing to Group III and IV. Histopathological examination confirmed the findings. In conclusion, nimodipine has a protective effect on ICP, CPP and infarct area whereas etomidate has limited and lidocaine no effect at all.Item Effects of gabapentin, carbamazepine, and CNQX on cognitive functions and behavior in rats with pilocarpine-induced status epilepticus(Wiley, 2010-06) Güleç, Güliz Uyar; Büyükcoşkun, Naciye İşbil; Kahveci, Nevzat; Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı.; AAH-1692-2021; AAG-7070-2021Item Effects of intracerebroventricularly-injected morphine on anxiety, memory retrieval and locomotor activity in rats: Involvement of vasopressinergic system and nitric oxide pathway(Pergamon-Elsevier Science, 2006) Kahveci, Nevzat; Güleç, Güldal; Özlük, Kasım; Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı.; 0000-0003-0863-1547; 0000-0003-0841-8201; C-5730-2015; AAG-7070-2021; 6602597846; 6602752303; 6602676331Morphine has been shown to alter several behavioural processes. We aimed to investigate the effects of intracerebroventricular (i.c.v.) morphine on anxiety, memory retrieval and locomotor activity in rats and to elucidate the possible involvement of the vasopressinergic system and the nitric oxide (NO) pathway in these effects. Rats were pretreated with morphine (0.5, 5, 50 mu g/5 mu l; i.c.v.) or saline (5 mu l; i.c.v.) 30 min before the elevated plus maze test, the probe trial of the Morris water maze and the open field test. Morphine (5 mu g/5 mu l; i.c.v.) induced significant anxiolytic effects in the elevated plus maze. None of the doses of morphine produced any effects in the probe trial of the Morris water maze and the open field. Pretreatment with an arginine vasopressin (AVP) V-1 receptor antagonist (25, 125 ng/5 mu l; i.c.v.), an AVP V-2 receptor antagonist (25, 125 ng/5 mu l, i.c.v.), or L-NAME, an NO synthase inhibitor (5, 25 mu g/5 mu l; i.c.v.) 30 min before morphine significantly prevented the anxiolytic effects of morphine. These results suggest that i.c.v. morphine has significant anxiolytic effects, probably mediated by both vasopressinergic system and NO pathway, but has no effect on memory retrieval or locomotor activity, at least at the applied doses.Item Effects of intranigral vs intrastriatal fetal mesencephalic neural grafts on motor behavior disorders in a rat Parkinson model(Elsevier, 2005) Gören, Bülent; Kahveci, Nevzat; Eyigör, Özhan; Alkan, Tülin; Korfalı, Ender; Özlük, Kasım; Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Beyin Cerrahisi Anabilim Dalı.; 0000-0003-3463-7483; 0000-0003-0841-8201; ABE-5128-2020; AAH-1718-2021; AAG-7070-2021; 6602543716; 6602597846; 6603109907; 6601953747; 7004641343; 6602676331Background: Numerous experimental and clinical studies have shown that intrastriatal fetal mesencephalic grafts grow, survive, and reinnervate host brain tissue, resulting in partial recovery of motor deficits. In addition, pharmacological evidence indicates that these grafts increase dopamine secretion in lesioned brain. However, to date, no grafting method has completely restored the nigrostriatal pathway, and there is no consensus on optimal graft numbers or locations. This study compared outcomes with multiple striatal grafts vs a single intranigral graft in a rat model of Parkinson disease. Methods: Forty-one female Wistar rats weighing 200 to 250 g were used. First, baseline rotational behavior testing with amphetamine injection was done to identify each animal's dominant nigrostriatal pathway (left vs right hemisphere). Some rats then received a unilateral intranigral injection of 6-hydroxydopamine (4 mu L [8 mu g]) to produce the Parkinson model lesion, and rotational testing was repeated. One group of the lesioned rats received a single intranigral injection of suspended fetal ventral mesencephalic cells (n = 11), and another received multiple intrastriatal grafts of the same type (n = 11). Results: Both grafted groups showed significant improvement on rotational testing with amphetamine and apomorphine at 6 weeks "postgrafting" (P <.001 for "postlesioning" vs postgrafting results in each of the 2 groups); however, the animals with multiple intrastriatal grafts showed complete recovery from motor asymmetry, whereas the rats with single intranigral grafts showed only partial improvement. Conclusion: The findings indicate that multiple intrastriatal grafts result in significantly greater functional improvement than single intranigral grafts in this rat Parkinson model.Item Experimental subarachnoid haemorrhage models in rats(Springer-Verlag Wien, 2002) Kanpolat, Y.; Alkan, Tülin; Korfalı, Ender; Kahveci, Nevzat; Uludağ Üniversitesi/Tıp Fakültesi/Nöroloji Anabilim Dalı.; 0000-0003-0841-8201; AAG-7070-2021; 6601953747; 7004641343; 6602597846There is no comprehensive and reliable model available in small animals that are suitable for the study of subarachnoid haemorrhage (SAH). In the study we reviewed the advantages and disadvantages of available SAH models in rats and presented our model. Experimental SAH was induced in a group of 350-450 g SpragueDawley rats. A 2 mm-diameter burr hole was drilled and, working under a microscope, haemorrhage was produced by transclival puncture of the basilar artery with a 20 mum thick piece of glass. The rats were assigned to either the experimental group (n: 7) or the control group (n: 7). Local cerebral blood flow (LCBF), intracranial pressure (ICP), and cerebral perfusion pressure (CPP) were measured for 60 min after SAH, after which the rats were decapitated. Microscopic examinations were done on three different segments of the basilar artery. There was a significant and sharp drop in LCBF just after SAH was induced (56.17 +/- 12.80 mILD/min/100 g and 13.57 +/- 5.85 mILD/min/100 g for baseline and post-SAH, respectively; p < 0.001), the flow slowly increased by the end of the experiment but never recovered to pre-SAH values (43,63 +/- 7.6 mILD/min/ 100 g, p < 0.05). ICP (baseline 7.33 +/- 0.8 mmHg) increased acutely to 70.6 +/- 9.2 mmHg, and also returned to normal levels by 60 min after SAH. CPP (baseline 75.1 +/- 4.9 mmHg) dropped accordingly (to 21.0 +/- 6.3 mmHg) and then increased, reaching 70.1 +/- 4.9 mmHg at 60 min after SAH. Examinations of the arteries revealed decreased inner luminal diameter and distortion of the elastica layer. We present an inexpensive and reliable model of SAH in the rat that allows single and multiple haemorrhages and to study the early and late course of pathological changes.Item The heat shock protein 32 response to intestinal ischemia and ischemia/reperfusion injury(Oxford University, 2010-06) Öztürk, Erman; Kahveci, Nevzat; Oral, Arzu Yılmaztepe; Özlük, Kasım; Yılmazlar, Tuncay; Uludağ Üniversitesi/Tıp Fakültesi/Genel Cerrahi Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyokimya Anabilim Dalı.; AAG-7070-2021
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