Browsing by Author "Koç, A. Filiz"

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    Loss-of-function mutations in PNPLA6 encoding neuropathy target esterase underlie pubertal failure and neurological deficits in gordon holmes syndrome
    (Endocrine Soc, 2014-10-01) Topaloğlu, A. Kemal; Lomniczi, Alejandro; Kretzschmar, Doris; Dissen, Gregory A.; Kotan, L. Damla; McArdle, Craig A.; Koç, A. Filiz; Hamel, Ben C.; Güçlü, Metin; Papatya, Esra D.; Eren, Erdal; Mengen, Eda; Gürbüz, Fatih; Cook, Mandy; Castellano, Juan M.; Kekil, M. Burcu; Mungan, Neslihan O.; Yüksel, Bilgin; Ojeda, Sergio R.; Güçlü, Metin; Papatya, Esra D.; EREN, ERDAL; Uludağ Üniversitesi/Tıp Fakültesi/Pediatrik Endokrinoloji ve Metabolizma Anabilim Dalı; Uludağ Üniversitesi/Tıp Fakültesi/Endokrinoloji ve Metabolizma Anabilim Dalı; 0000-0002-1684-1053; 0000-0003-4664-7435; ABI-4847-2020; GQO-9634-2022; JPK-3909-2023
    Context: Gordon Holmes syndrome (GHS) is characterized by cerebellar ataxia/atrophy and normosmic hypogonadotropic hypogonadism (nHH). The underlying pathophysiology of this combined neurodegeneration and nHH remains unknown.Objective: We aimed to provide insight into the disease mechanism in GHS.Methods: We studied a cohort of 6 multiplex families with GHS through autozygosity mapping and whole-exome sequencing.Results: We identified 6 patients from 3 independent families carrying loss-of-function mutations in PNPLA6, which encodes neuropathy target esterase (NTE), a lysophospholipase that maintains intracellular phospholipid homeostasis by converting lysophosphatidylcholine to glycerophosphocholine. Wild-type PNPLA6, but not PNPLA6 bearing these mutations, rescued a well-established Drosophila neurodegenerative phenotype caused by the absence of sws, the fly ortholog of mammalian PNPLA6. Inhibition of NTE activity in the L beta T2 gonadotrope cell line diminished LH response to GnRH by reducing GnRH-stimulated LH exocytosis, without affecting GnRH receptor signaling or LH beta synthesis.Conclusion: These results suggest that NTE-dependent alteration of phospholipid homeostasis in GHS causes both neurodegeneration and impaired LH release from pituitary gonadotropes, leading to nHH.