Browsing by Author "Malyer, Hulusi M."
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Item Aristolochia adalica (Aristolochiaceae), a new species from Anatolia, Turkey(Finnish Zoological Botanical Publishing Board, 2014-03-07) Tosunoǧlu, Aycan; Malyer, Hulusi M.; Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Biyoloji Bölümü.; 0000-0003-2303-672X; 35235434000; 6602736554Aristolochia adalica Tosunoglu & Malyer sp. nova (Aristolochiaceae) is described and illustrated from southwest Anatolia, Turkey. It is morphologically close to the East Aegean endemic A. hirta; however, these species differ in some important characteristics, such as the perianth and leaf shapes and sizes, indumentum of the limb, and seed and pollen morphology. The taxonomic relationships are discussed.Item Determination of phenolic compounds in Prunella L. by liquid chromatography-diode array detection(Elsevier, 2011-07-15) Şahin, Saliha; Demir, Cevdet; Malyer, Hulusi M.; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Anabilim Dalı.; 0000-0003-1508-0181; 0000-0002-9381-0410; AAH-2892-2021; ABA-2005-2020; 15027401600; 7003565902; 6602736554Four species of Prunella L. (Prunella vulgaris L., Prunella laciniata L., Prunella grand:flora L. and Prunella orientalis Bornm.) belong to the family of Lamiaceae and representing popular Western and Chinese herbal medicine were examined for the content of phenolic compounds. Phenolic acids (rosmarinic acid, caffeic acid, ferulic acid, chlorogenic acid, protocatechuic acid), flavonoids (rutin, quercetin) in different quantitative proportions depending on extracts were determined by the rapid, selective and accurate method combining solvent/acid hydrolysis extraction and high performance liquid chromatographydiode array detection (HPLC-DAD). Water, methanol, butanol, acetonitrile, ethyl acetate, hexane and their acidic solutions were used to examine the efficiency of different solvent systems for the extraction of phenolic compounds. Acid hydrolysis extraction was established as the most suitable extraction method for phenolic compounds.Item Determination of total phenolic content of Prunella L. by immobilized enzyme bioreactor(Royal Soc Chemistry, 2011-04) Şahin, Saliha; Demir, Cevdet; Malyer, Hulusi M.; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Anabilim Dalı.; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Anabilim Dalı.; 0000-0003-1508-0181; 0000-0002-9381-0410; ABA-2005-2020; AAH-2892-2021; 42960979100; 7003565902; 6602736554A new method for determination of total phenolic content of four Prunella L. species was developed by immobilizing horseradish peroxidase (HRP) on styrene-divinylbenzene-polygluteraldehyde (STY-DVB- PGA) polymer. The method is based on the spectrophotometric measurement of the final quinone-imine colored product, absorbing at 510 nm, by horseradish peroxidase oxidation in presence of hydrogen peroxide. The effects of solvents were investigated for the extraction of phenolic compounds in Prunella L. plants. HRP was covalently attached on the STY-DVB-PGA with 90% efficiency at optimum immobilization conditions. The total phenol contents ranged from 4 to 152 mg GAE per g of dried sample for pure solvents and ranged from 19 to 763 mg GAE per g of dried sample for acidic extracts using the immobilized enzymatic method. STY-DVB-PGA beads are a good potential support for the immobilization of HRP enzyme and can be used for determination of the total phenolic content of plants. In the analysis of four Prunella L. species, high correlations were obtained between enzymatic and Folin methods, supporting the validity of the enzymatic method proposed. The enzymatic (free/immobilized) methods are more specific and rapid than the Folin method. Acidic solvents are more effective than pure solvents for extraction of phenolic compounds in Prunella L. species.