Browsing by Author "Nak, Y."

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Ascorbic acid inclusion in semen extender improves the post-thawed semen quality of sahiwal cattle (Bos indicus)
(Zoological Society of Pakistan, 2015-12-01) Sohail, M. U.; Shahzad, A. H.; Iqbal, S.; Shabbir, M. Z.; Iqbal, Z.; Abbas, S.; Younus, M.; Nak, D.; Nak, Y.; Arshaad, T.; NAK, DENİZ; NAK, YAVUZ; FPJ-3706-2022; AAH-5494-2021
The antioxidant effects of ascorbic acid at different inclusion rates were evaluated upon semen (n=150) from mature Sahiwal cattle bulls (Bos indicus, n = 6) in summer season. The semen was diluted in Tris-citrate egg yolk extender containing different levels (0.0, 1.0, 2.0, 3.0, and 4.0 mg/mL) of ascorbic acid at 37.0 degrees C. Semen was cryopreserved at -196 degrees C in 0.5 mL French straws. Semen straws were thawed at 37 degrees C to assess the spermatozoa indices in terms of motility, viability, plasma membrane and acrosomal integrity under phase-contrast microscope. Supravital staining, hypo-osmotic swelling test and normal acrosomal reaction analysis tests were also performed for viability, plasma membrane and acrosomal integrity, respectively. The data were subjected to one way ANOVA. The results revealed significant improvement (P< 0.05) in post thaw sperm quality in terms of motility, vitality, acrosomal and plasma membrane integrity by the increasing concentrations of ascorbic acid (1.0, 2.0, 3.0, 4.0 mg/mL) in semen extender. Quality parameters were higher (P< 0.05) when 3.0 mg/mL of ascorbic acid was added in semen extender followed by 2.0 and 4.0 mg/mL. In conclusion the addition of ascorbic acid at the rate of 3.0 mg/mL in the semen extender may improve the semen quality of Sahiwal cattle bull.
Effects of carprofen and/or cidr administration on pregnancies per artificial insemination around pregnancy recognition in lactating dairy cows
(Hellenic Veterinary Medical, 2021-07-01) Abay, M.; Pancarci, S. M.; Güngör, O.; Alparslan, D.; Bekyurek, T.; Nak, Y.; Bursa Uludağ Üniversitesi/Veterinerlik Fakültesi.
Effect of carprofen and/or CIDR on pregnancies per AI (P/AI) 14 days after artificial insemination (AI) in lactating cows were investigated. Following detection of corpus luteum (CL) by ultrasonography (USG) 14 days after AI, cows (n=853) were randomly allocated to CARP (carprofen, 1.4 mg/kg; Rimadyl (R) XL), CIDR (progesterone, 1.38g, for seven days), CARP+CIDR and CONT (control) groups. CL was re-determined with USG 21 days after AI to monitored the maintenance of CL. Rates of maintenance of CL did not differed among CARP (79.6% [168/211]), CIDR (86.0% [196/228]), CARP+CIDR (80.0% [172/215]) and CONT (74.9% [149/199]) groups; however, the interaction effect of treatment by body condition score (BCS) at AI (P<0.05) were existed. In this matter, among cows with BCS <= 2.5, chances of maintenance of CL was lower in CONT (70.7%, [111/157]) group compared to those in CARP (81.7%, [125/153]), CIDR (83.6%, [153/183]) and CARP+CIDR (80.1%, [129/161]) groups. Whereas, among cows with BCS>2.5, chances of maintenance of CL were lower in CARP (74.1%, [43/58]) and CARP+CIDR (79.6%, [43/54]) groups compared to those in CONT (90.5%, [38/42]) and CIDR (95.6%, [43/45]) groups. For the P/AI at 28-32 days after AI, there was no difference among CARP (48.8% [103/211]), CIDR (50.9 [116/228]), CARP+CIDR (47.4% [102/215]) and CONT (44.7% [89/199]) groups. Pregnancy losses between 28-32 and 55-60 days did not differ among CARP (3.9%, [4/103]), CIDR (4.3%, [5/116]), CARP+CIDR (5.9%, [6/102]) and CONT (6.7%, [6/89]) groups. However, there was a significant (P<0.05) interaction effect of treatment by the number of services on pregnancy losses. In this regard, pregnancy losses were higher in cows inseminated thrice and four or more times in CONT (11.8% and 16.7%) group compared to those in CARP (0% and 4.8%), CIDR (0% and 6.9%), CARP+CIDR (0% and 11.1%); respectively. Consequently, no effects of carprofen or CIDR around pregnancy recognition on P/AI were observed despite a higher maintenance rate of CL in lactating cattle. Furthermore, fewer pregnancy losses in cows following three or more services could indicate the beneficial carry-over effects of carprofen and/or CIDR administration around maternal recognition of pregnancy.
Evaluation of serum analytes in pregnant and non-pregnant dairy cows as indicators of pregnancy
(South African Journal Of Animal Sciences, 2020-01-01) Abbas, S.; Yousaf, M. S.; Ahmad, I; Rehman, H.; Mahmood, A. K.; Ashraf, S.; Blouch, R. S.; Shahzad, A. H.; Nak, Y.; Nak, Yavuz; NAK, YAVUZ; Bursa Uludağ Üniversitesi/Veteriner Fakültesi; AAH-5494-2021
This study was conducted to reveal the dynamics of haematological indicators in pregnant and nonpregnant dairy cows. Sixty multiparous healthy dairy cows were divided into four groups based on the length of time they had been pregnant, namely first, second and third trimesters, and non-pregnant (n=15 each). Blood was collected from each animal, and serum was harvested and stored at -20 degrees C for biochemical profiling. Concentrations of serum total oxidants (TOC), ceruloplasmin oxidase (CpO) and triiodothyronine (T-3) were higher (P <0.05) during the third trimester compared with non-pregnant cows. Serum arylesterase (Ary) concentration was lower (P <0.05) during the second and the third trimesters compared with the non-pregnant cows. The concentration of serum total homocysteine (tHcy) was higher (P <0.05) the third trimester compared with the first and the second trimesters. The concentrations of serum total antioxidants (TAC), paraoxonase 1 (PON1), thyroxine (T-4), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were not different in the pregnant and the non-pregnant cows. Thus, TOC, Ary, CpO, tHcy and T3 could be taken as biological markers to assess the progression of pregnancy and to develop management tools to improve health status during late gestation in dairy cows.
Sry-negative xx sex reversal in an english cocker spaniel: A case report
(Czech Academy Agricultural Sciences, 2015-01-01) Shahzad, A. H.; Nak, D.; NAK, DENİZ; Gülten, T.; Karkucak, M.; Yılmaz, R.; Nak, Y.; NAK, YAVUZ; Şimşek, G.; Bursa Uludağ Üniversitesi/Veterinerlik Fakültesi; 0000-0002-0944-2230; 0000-0002-8623-8292; ABG-8940-2020; AAH-5494-2021
A SRY-negative XX male describes a female karyotype with testicular tissue resulting from abnormal gonadal development. In such cases male gonad formation takes place in the absence of a Y chromosome which supports the hypothesis that some other genes are also responsible for testicular development. The present case report describes a one-year-old dog brought to the hospital with a complaint of no oestrus cycle. Apparently normal masculine bitch had an enlarged clitoris with an os on radiograph. A gonado-hysterectomy was performed. Instead of ovarian structures both testicular masses were present and a normal complete bicornuate uterus with a normal cervix was observed. The histology of both gonads showed two testes, each with its ductus deferens and the uterus with typical histological structure. Cytogenetic evaluation of peripheral blood lymphocytes revealed a female with a normal karyotype (78, XX). Polymerase chain reaction (PCR) analysis of genomic DNA showed that the SRY gene was absent.