Browsing by Author "Sharifi, Ahmad"
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Item Carnation etched ring virus elimination through shoot tip culture(Uludağ Üniversitesi, 2012) Ashnayi, Mahnaz; Kharrazi, Mahdiyeh; Sharifi, Ahmad; Mehrvar, MohsenCarnation etched ring virus (CERV) is the second most destructive virus which infects carnation and the only DNA virus among infecting viruses of carnation. In symptomatic leaves of carnation consist of mottling, necrotic and chlorotic flecks or blotches. Virus was detected by DAS-ELISA and PCR. Treatments consisted of different sizes of meristem and MS medium supplemented with different plant growth regulators (PGRS) (0.5 mg/l benzyl adenine (BA), 0.5 mg/l gibberellic acid (GA3) and medium without PGRS.). The plantlets were analysed by PCR in order to evaluate virus eradication. Results of PCR in vitro culture revealed that explant size and type of PGRS had a significant effect on elimination of CERV and the highest amount of it (100%) was observed on medium containing BA in meristem size of 0.4, 0.7 mm and the lowest amount of it (26%) was occurred on medium supplemented with GA3 in meristem size of 1mm. So far, there is no reporting about influence of PGRS on elimination of viruses.Item Disinfecting effects of nano silver fluids in gerbera (Gerbera jamesonii) capitulum tissue culture(Uludağ Üniversitesi, 2012) Fakhrfeshani, Masoud; Bagheri, Abdolreza; Sharifi, AhmadMicrobial contamination is one of the most important problems in plant tissue culture and various methods are employed to reduce it. Microbial contamination is an important barrier in the development of Gerbera tissue culture and micropropagation procedure even after disinfecting with normal methods. In this experiment the antifungal and antibacterial activity of nano-silver fluids was investigated in gerbera tissue culture and the effects of four different nano-silver concentration (25, 50, 100 and 200mg L-1) in four soaking time of explants (15, 30, 60 and 180 min) were compared with two control treatments that included disinfecting explants with soaking them in Sodium Hypochlorite and Sodium Hypochlorite followed by Mercury Chloride aqueous solution. The explants were cultured on dedicated MS medium to evaluate the effects of NS on viability and other apparent properties. The analysis of variance resulted that there are significant differences in contamination rate, both among treatments and between control and treatments. The 200 mg L-1 nano-silver solution had successfully controlled bacterial and fungal contamination and had no undesired effects on regeneration of plantlets. According to reports on high levels of contamination in Gerbera tissue culture and also the hazardous environmental effects of mercury chloride, so nano-silver solution can be used as a low risk fungicide and bactericide in Gerbera capitulum tissue culture.Item In vitro culture of carnation (Dianthus caryophyllus L.) focusing on the problem of vitrification(Uludağ Üniversitesi, 2011) Kharrazi, Mahdiyeh; Nemati, Hossein; Tehranifar, Ali; Bagheri, Abdolreza; Sharifi, AhmadThis study was conducted to evaluate the effect of plant growth regulators on in vitro shoot multiplication, vitrification and rooting of two carnation cultivars (Eskimo Mogr and Innove Orange Bogr). Isolated axillary buds were cultured on MS medium supplemented with different levels of Benzyl amino purine (BAP) or kinetin (Kin) in combination with 0.2 mg/l NAA and shoot multiplication and vitrification rate were tested. Then different concentrations of Naphthalene acetic acid (NAA) or Indole butyric acid (IBA) on root induction of regenerated shoots were surveyed. Shoot regeneration and vitrification were influenced by the cultivar, kind of cytokinin and its concentration. Eskimo Mogr produced more shoots in comparison with Innove Orange Bogr (6.17 and 3.83 shoots/explant, respectively). BAP-containing media had more shoot multiplication and vitrification than Kin and by increasing the concentration of cytokinin, shoot multiplication and vitrification increased. There was a negative correlation between plant height and internode length with vitrification (-77% and -85%, respectively). By considering the amount of multiplication and rate of vitrification for obtaining the highest number of normal shoots, MS medium containing lower concentration of BAP (1mg/l for Eskimo Mogr and 2 mg/l for Innove Orange Bogr) and 0.2 mg/l NAA is recommended. Rooting of regenerated shoots was dependent on the cultivar. The highest root percentage for Eskimo Mogr (83%) was achieved on the medium supplemented with 0.5 mg/l NAA, while for Innove Orange Bogr (98%) it was obtained on the medium containing 1.5 mg/l IBA.