Browsing by Author "Sorucu, Ali"
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Item Bursa’da çiğ süt ve uht sütlerde aflatoksin m1 düzeyleri(Uludağ Üniversitesi, 2011-09-30) Oruç, H. Hüseyin; Temelli, Seran; Sorucu, Ali; Uludağ Üniversitesi/Veteriner FakültesiBu çalışmada, Bursa’da süt sığırcılığı işletmelerinden toplanan çiğ sütler ile perakende satışa sunulan UHT sütlerdeki aflatoksin M1 (AFM1) düzeylerinin belirlenmesi ve elde edilen sonuçların halk sağlığı yönünden değerlendirilmesi amaçlanmıştır. Bu amaçla, 30 adet çiğ süt ve 54 adet UHT süt olmak üzere toplam 84 adet süt örneğinde AFM1 düzeyleri ELISA tekniği ile belirlenmiştir. İncelenen çiğ süt ve UHT süt örneklerinin tümünde AFM1 tespit edilmiş olup (% 100.0), ortalama AFM1 miktarı 6.40±0.42 ng/kg olarak saptanmıştır. Sonuç olarak, süt örneklerinde bulunan AFM1 düzeylerinin Türk Gıda Kodeksi, AB ve FAO/WHO’nun tolerans limitlerinin oldukça altında olduğu ve halk sağlığı açısından herhangi bir risk oluşturmadığı tespit edilmiştirPublication Characterization of commercially available propolis products in Turkey based on individual phenolic compounds(Taylor & Francis, 2021-06-14) Oruç, Hasan Hüseyin; Çaycı, Meltem; Sorucu, Ali; Uzabacı, Ender; Nyandwi, Ramadhan; ORUÇ, HASAN HÜSEYİN; Çaycı, Meltem; UZABACI, ENDER; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Farmakoloji ve Toksikoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Biyoistatistik Anabilim Dalı.; 0000-0002-5399-2395; 0000-0002-3401-9186; 0000-0002-9634-0055; 0000-0003-4371-425X; JMP-9327-2023; JPL-5273-2023; GBO-2873-2022The healing properties of propolis, such as antibacterial, antiviral, antifungal, anti-inflammatory, antitumoral, antioxidant, immunomodulatory, tissue regeneration, and anti-ulcer, are due to the high content of phenolic compounds (flavonoids and phenolic acids). Therefore, propolis can potentially be used for human consumption or even for medicinal purposes. This study aimed to analyze sixteen individual phenolic compounds that have beneficial effects, determine their concentrations in local and imported raw propolis and products consumed in Turkey, and evaluate the determined results for propolis quality. A total 91 propolis samples were collected from commercial raw propolis and propolis products, which were from different provinces of Turkey and different countries, sold and used in Turkey between 2015 and 2018. Sixteen phenolic compounds were analyzed by high-performance liquid chromatography with a diode array detector system. Regardless of their origin, the results indicated that the concentrations of individual phenolic compounds varied greatly within crude, ethanol-based, water-based, and propylene glycol propolis samples analysed. There were significant differences in some individual phenolic compounds between the local and imported samples (p < 0.05). The current results showed that qualitative and quantitative analysis of commercial propolis samples for useful phenolic compounds are important and may provide quality control of propolis products.Item Determination of acute oral toxicity of flumethrin in honey bees(Oxford University Press, 2012-12) Hranitz, John M.; Duell, Meghan E.; Oruç, Hasan Hüseyin; Sorucu, Ali; Çakmak, İbrahim; Aydın, Levent; Orman, Abdülkadir; Uludağ Üniversitesi/Arıcılığı Geliştirme-Uygulama ve Araştırma Merkezi.; Uludağ Üniversitesi/Veteriner Fakültesi/Farmakoloji ve Toksikoloji Anabilim Dalı.; Uludağ Üniversitesi/Mustafakemalpaşa Meslek Yüksekokulu.; Uludağ Üniversitesi/Veteriner Fakültesi/Parazitoloji Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Zooteknik Anabilim Dalı.; 0000-0001-9138-4422; 0000-0002-5399-2395; AAG-9134-2021; AAH-2558-2021; AAI-2212-2021; 55944769400; 55236493200; 57207796431; 55808198600; 24335834100Flumethrin is one of many pesticides used for the control and treatment of varroatosis in honey bees and for the control of mosquitoes and ticks in the environment. For the control of varroatosis, flumethrin is applied to hives formulated as a plastic strip for several weeks. During this time, honey bees are treated topically with flumethrin, and hive products may accumulate the pesticide. Honey bees may indirectly ingest flumethrin through hygienic behaviors during the application period and receive low doses of flumethrin through comb wax remodeling after the application period. The goal of our study was to determine the acute oral toxicity of flumethrin and observe the acute effects on motor coordination in honey bees (Apis mellifera anatoliaca). Six doses (between 0.125 and 4.000 mu g per bee) in a geometric series were studied. The acute oral LD50 of flumethrin was determined to be 0.527 and 0.178 mu g per bee (n = 210, 95% CI) for 24 and 48 h, respectively. Orally administered flumethrin is highly toxic to honey bees. Oral flumethrin disrupted the motor coordination of honey bees. Honey bees that ingested flumethrin exhibited convulsions in the antennae, legs, and wings at low doses. At higher doses, partial and total paralysis in the antennae, legs, wings, proboscises, bodies, and twitches in the antennae and legs were observed.Publication Determination of biologically active phenolic compounds in propolis by LC-MS/MS according to seasons and altitudes(Springer, 2019-09-01) Sorucu, Ali; Oruç, Hasan Hüseyin; ORUÇ, HASAN HÜSEYİN; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Farmakoloji ve Toksikoloji Anabilim Dalı; 0000-0002-5399-2395; AAI-2212-2021The present study aimed to determine the effect of season and altitude to amount and prevalence of phenolic compounds in propolis. For the aim, 174 propolis samples were collected from 58 stationary apiaries from altitudes of 0-200 m, 200-500 m and above 500 m in spring, summer, and autumn distributed in the Marmara region of Turkey. Ethanol-extracted propolis samples were analyzed by LC-MS/MS to determine nine flavonoids and seven phenolic acids. Significant differences were found in average levels of galangin, naringenin, pinocembrin, quercetin, luteolin, caffeic acid, trans-cinnamic acid, p-coumaric acid, m-coumaric acid, and CAPE across the seasons and altitudes (P < 0.05). However, no differences were found levels of rutin, apigenin, kaempferol, ferulic acid and gallic acid (P < 0.05). Most of the significant differences were found between spring and autumn (10 differences). The prevalence of flavonoids was generally lower than phenolic acids. CAPE and pinocembrin had majority of the significant differences in phenolic compounds according to season and altitude. The prevalence of pinocembrin 100%, m-coumaric acid 99.4%, CAPE 99.4%, naringenin 99.4%, caffeic acid 98.9%, p-coumaric acid 98.3%, quercetin 96% and luteolin 95.4% were determined that dominant phenolic compound of Marmara Region of Turkey's propolis. Furthermore, Salix spp. 75.8%, Quercus spp. 74.4%, Populus spp. 72.4%, and Pinus spp. 53.5% were mainly determined source of propolis around the apiaries. These results show that different sampling of seasons and altitudes effect chemical composition of propolis even from the same area. The study indicated that seasons and altitudes are important factors for standardization of propolis.Item Determination of the effect of green extraction solvents on the phenolic acids and flavonoids of propolis(Bursa Uludağ Üniversitesi, 2022-07-26) Kekeçoğlu, Meral; Sorucu, AliPropolis is an important bee product with many biological activities due to its containing phenolic compounds. The phenolic compounds of propolis vary depending on the plant source, season, altitude, extraction method and solvent. The present study investigated the extraction of phenolic compounds from propolis according to solvent factors. The propolis samples were extracted in four different solvents, which were water, ethanol-water (70%), dimethyl sulfoxide (DMSO), and L-lysine (8%), and were analyzed 36 phenolic compounds by HPLCDAD. Statistically significant differences in solubility of the phenolic compound at various levels were detected among the solvents (P <0.05). Only water and ethanol-water (70%) more successful than the other solvents were determined in the extraction of phenolic components of propolis. Phenolic acids generally dissolved higher in water, while flavonoids dissolved higher in ethanol were determined. Certain phenolic compounds were detected only in some of the propolis extract: syringic acid and daidzein in water, vitexin, rutin, and epigallocatechin in ethanol, and emodin in DMSO. Consequently, the chemical content is affected significantly depending on the extraction solvent of propolis. Therefore, it is essential to determine the extraction solvent and analyses of propolis before application for therapeutic purposes.Item Determination of various mycotoxin concentrations in the feedstuffs and feed produced by a feed manufacturer in Turkey(Kafkas Üniversitesi, 2012) Oruç, Hasan Hüseyin; Sorucu, Ali; Türkmen, İbrahim İsmet; Arslan, Erdem; Uludaǧ Üniversitesi/Veteriner Fakültesi/Farmakoloji ve Toksikoloji Anabilim Dalı.; Uludaǧ Üniversitesi/Saǧlik Bilimleri Enstitüsü.; Uludaǧ Üniversitesi/Veteriner Fakültesi/Hayvan Besleme ve Beslenme Hastalıkları Anabilim Dalı.; 0000-0002-5399-2395; AAI-2212-2021; 55944769400; 55236493200; 6701855392; 55236738400The aim of this study was to determine aflatoxin B, (AFB(1)), T-2 toxin, fumonisin, deoxynivalenol (DON) and zearalenone (ZEN) levels in various feedstuffs (local and imported feed materials) and feed produced in a feed manufacturer. In addition, aflatoxin M-1 (AFM(1)) levels were determined in raw cow milks (n = 24) collected from 10 certain dairy cattle farms. The samples consisted of dairy cattle (n = 26), fattening (n = 4), and calf starter (n = 4) feed, as well as feedstuffs (n = 40). All of the samples were analysed by ELISA. In the analysed samples, the incidence of AFB(1), AFM(1) and T-2 toxins, fumonisin, DON and ZEN were 100%, 80%, 75% and 33%, respectively. The results of this study in feedstuffs and feed revealed that the mycotoxin levels were lower than the mycotoxin limits of the European Union (EU) and Turkey. In previous studies, high concentrations of mycotoxins, especially AFB(1) and AFM(1), were identified in feedstuffs, feeds and milk in Turkey. In contrast, the mycotoxin levels in the current study were lower than that from the previous studies. The mycotoxin concentrations in imported feed materials (except fumonisin) were lower than those in feed materials obtained from Turkey. The results of this study indicate that the mean mycotoxin levels in feedstuff and feeds do not cause toxic effects in animals, and humans for AFM(1).Item Effects of season and altitude on biological active certain phenolic compounds levels and partial standardization of propolis(Ankara Üniversitesi, 2017) Ünal, Hasan Hüseyin; Oruç, Hasan Hüseyin; Sorucu, Ali; Aydın, Levent; Uludağ Üniversitesi/Veteriner Fakültesi/Farmakoloji ve Toksikoloji Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Parazitoloji Anabilim Dalı.; 0000-0002-5399-2395; AAI-2212-2021; 55944769400; 55236493200; 55808198600Propolis has antibacterial, antiviral, antifungal, anti-inflammatory, antitumoral, antioxidant, immunomodulatory, tissue regeneration, anti-ulcer, analgesic, local anaesthetic, and antiseptic effects related to its chemical components. The aim of the present study was to determine certain biological active phenolic compounds and their levels in propolis collected from Bursa province, and to evaluate propolis quality and effects of altitude and season. Propolis samples were collected using propolis traps from 15 different constant apiaries at varying altitudes (between 50 and 1000 m) located in Bursa province Turkey, during the spring, summer and autumn of 2012. Fourteen flavonoid and phenolic acids in ethanolic extracts were investigated by liquid chromatography tandem-mass spectrometry (LC-MS/MS). Galangin, naringenin, pinocembrin, caffeic acid and caffeic acid phenylethyl ester were detected in all samples (45 samples) for all seasons and altitudes. Significant differences were determined for certain phenolic compounds levels across altitudes and seasons. In the present study results showed that qualitative and quantitative analysis of propolis for useful phenolic compounds may provide partial standardization of propolis depend on altitude and season.Item In vitro phannacodynaniics of enrofloxacin against an Escherichia coli gyrA mutant(Medwell Online, 2011) Cengiz, Murat; Sorucu, Ali; Arslan, Erdem; Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji ve Toksikoloji Anabilim Dalı.; ABE-5935-2020; 16635026700; 55236738400; 55236493200The aim of this study was to investigate in vitro the pharmacodynamics of Enrofloxacin (ENR) against an Escherichia coli gyrA mutant (E. coli MT/128). Broth microdilution testing was used to determine the Minimum Inhibitory Concentration (MIC) and multi-step resistance selection was performed until reaching 1 mu g ML(-1) MIC. For time-kill experiments, colony counts were determined by plating each diluted sample onto Plate Count Agar and an integrated pharmacokinetic/pharmacodynamics area measure (log ratio area) was applied to all cfu data. A single C-T mutation was found in gyrA at codon 83. Concentration-dependent bacterial killing was observed for E. coli MT/128. Bactericidal activity for this strain was achieved within 4 h at concentrations >= 8 times the MIC with no significant regrowth by 24 h.Item Lethal and subethal effects of flumethrin (VAROSTOPA (R)) on the anatolian honey bee in the republic of Turkey(Oxford University, 2011-02) Duell, Meghan E.; Apted, Trent; Hall, N.; Albers, Leah M. Bates; Pendergraft, LomaJohn T.; Zuniga, Edier; Sorucu, Ali; Abramson, Charles, I; Barthell, John F.; Wells, Harrington; Hranitz, John M.; İkizoğlu, Didem; Selova, Semih; Aydın, Levent; Çakmak, İbrahim; Oruç, Hasan; Uludağ Üniversitesi/Veterinerlik Fakültesi/Veterinerlik Parazitolojisi Anabilim Dalı.; Uludağ Üniversitesi/Ziraat Fakültesi/Zootekni Bölümü.; Uludağ Üniversitesi/Veteriner Fakültesi/Farmakoloji ve Toksikoloji Anabilim Dalı.; 0000-0002-5399-2395; AAI-2212-2021Item Marmara bölgesindeki propolislerde biyolojik etkisi olan fenolik madde ve miktarlarının mevsim ve rakım farkına bağlı olarak belirlenmesi(Uludağ Üniversitesi, 2015-11-03) Sorucu, Ali; Oruç, Hasan Hüseyin; Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü/Veteriner Farmakoloji ve Toksikoloji Anabilim Dalı.Propolis, bal arılarının, bitkilerin reçine ve salgıları toplayıp balmumu, polen ve kendi enzimleri ile karıştırarak oluşturdukları önemli bir arı üründür. İçeriğinde 300 den fazla aktif bileşik bulundurur ve bu bileşikler propolise birçok biyolojik aktive kazandırır. Propolisin içeriğinde bulunan bu aktif bileşikler propolisin kaynağı olan bitkilere (kavak, söğüt vb.) ve propolisin toplandığı mevsime bağlı olarak değişir. Bu çalışmada, Marmara bölgesinde 0-200 m, 200-500 m ile 500 m ve üzeri yüksekliklerde belirlenen 58 arılıktan, ilkbahar, yaz ve sonbahar mevsimlerinde, toplam174 propolis numunesi toplandı. Bu propolislerin % 70'lik etil alkol/su ile ekstraksiyonu yapıldı. Propolis numunelerinde galangin, kuersetin, kaemferol, rutin, naringenin, pinosembrin, apigenin, luteolin flavonoidleri ve kafeik asit, ferulik asit, sinamik asit, m-kumarik asit, p-kumarik asit, kafeik asit fenetil ester (CAPE), gallik asit fenolik asitleri LC-MS/MS sistemi ile analiz edildi. Galangin, naringenin, pinosembrin, kuersetin, luteolin, kafeik asit, sinamik asit, m-kumarik asit, p-kumarik asit ve CAPE fenolik bileşiklerin ortalamalarının mevsim ve yüksekliğe bağlı istatistiki olarak anlamlı düzeyde değiştiği fakat rutin, apigenin, kaemferol, ferulik asit ve gallik asit, fenolik bileşiklerin etkilenmediği belirlendi. Marmara bölgesi propolislerinin ana fenolik bileşikleri; pinosembrin, naringenin, m-kumarik asit, p-kumarik asit, kafeik asit ve CAPE'in olduğu belirlendi. Araştırmacılar propolisin genel standardizasyonun mümkün olmadığını, fakat bölgesel standardizasyonun yapılabileceğini bildirmektedir. Bu araştırma ile aynı bölgede yüksekliğin ve mevsimin propolis içeriğindeki fenolik bileşikleri etkilediği bu nedenle bölgesel standardizasyon yerine aynı bölgede mevsime ve yüksekliğe göre standardizasyonun yapılmasının daha doğru olacağı kanısına varıldı. Propolisin içeriğinde bulunan aktif bileşiklerin belirlenerek bu bileşiklerin biyolojik etkileri doğrultusunda propolisin ticari ürüne dönüştürülmesi ve içeriği bilinen propolislerin bilimsel çalışmalarda kullanılımasının daha faydalı olacağı belirlendi.Item Propolisin sağlık açısından önemi, kalitesinin belirlenmesi ve Türkiye açısından irdelenmesi(Uludağ Üniversitesi, 2013-04-15) Oruç, Hasan Hüseyin; Sorucu, Ali; Aydın, Levent; Uludağ Üniversitesi/Veteriner Fakültesi.; Uludağ Üniversitesi/Arıcılık Geliştirme Uygulama ve Araştırma Merkezi.Propolisin içinde 300’den fazla aktif madde bulunmaktadır. Bunların içinde insan sağlığı açısından önemli olanlar fenolik bileşiklerdir ve fenolik bileşiklerden de özellikle bazı fenolik asitler ve flavanoidlerdir. Kaynağına ve dolayısıyla içeriğine bağlı olarak, propolisin antimikrobiyal (bakteri, virüs, parazit ve mantarlara karşı), antioksidan, antiinflamatuar, antikanser ve ülser önleyici gibi etkileri bulunmaktadır. Bu etkiler propolisin içeriğine bağlı olarak değişebilmekte ve yine içeriğine bağlı olarak propolisin zararlı etkileri de görülebilmektedir. Bu nedenle propolislerin bilinçli toplanması, uygun bir şekilde ekstraktlarının hazırlanması, içerik analizlerinin yapılması ve tüketime sunulması gerekir. Bu şekilde hazırlanan propolis ekstraklarının kaliteleri belirlenebilecek ve belirli bir oranda standardize edilebilecektir. Böylece tüketime sunulan propolislerin içindeki yararlı maddeler ve miktarlarının bilinmesiyle istenilen yararlı etkilerinin görülmesine katkı sağlanmış olacaktır. Bu anlamda Türkiye’deki propolislerin de ele alınması ve içerik miktarları açısından irdelenmesi gerekmektedir.