Browsing by Author "Tvarijonaviciute, Asta"
Now showing 1 - 11 of 11
- Results Per Page
- Sort Options
Item Adiponectin and IGF-1 are negative acute phase proteins in a dog model of acute endotoxaemia(Elsevier, 2011-03-15) Tvarijonaviciute, Asta; Ceron, Jose J.; Eralp, Oya İnan; Kocatürk, Meriç; Yılmaz, Zeki; Uludağ Üniversitesi/Veterinerlik Fakültesi/Dahiliye Anabilim Dalı.; 0000-0002-4242-8609; 0000-0001-9836-0749; AAG-2943-2020; 24472964600; 36437200800; 35944810500The objective of this study was to evaluate the influence of an experimentally induced acute inflammation on serum adiponectin and insulin-like growth factor 1 (IGF-1) levels in the dog, and to compare their evolution with other well-established acute phase proteins (APPs) such as C-reactive protein (CRP), and haptoglobin (Hp). Therefore levels of adiponectin, IGF-1 and a profile of APPs were measured in healthy dogs after intravenous administration of E. coli LPS (0.02 mg/kg) and compared with dogs injected with saline solution (0.2 mL/kg). Adiponectin and IGF-1 were both decreased in response to endotoxins in the dog. Significant positive correlations were found between adiponectin and IGF-1 (r = 0.31; p < 0.05). Adiponectin had also a significant negative correlation with CRP (r = 0.39; p < 0.05) and Hp (r = -0.27; p < 0.05), whereas IGF-1 had significant negative correlation with CRP (r = -0.52; p < 0.001). The results obtained in the present study indicate that adiponectin and IGF-1 behave as negative acute phase proteins after acute inflammatory stimulus in dogs.Item Changes in salivary analytes in canine parvovirus: A high-resolution quantitative proteomic study(Elsevier, 2018-09-18) Franco-Martínez, Lorena; Tvarijonaviciute, Asta; Horvatić, Anita; Guillemin, Nicolas; Cerón, José Joaquín; Escribano, Damián; Eckersall, David; Lamy, Elsa; Martínez-Subiela, Silvia; Mrljak, Vladimir; Kocatürk, Meriç; Yılmaz, Zeki; Uludağ Üniversitesi/Veterinerlik Fakültesi/Dahiliye Anabilim Dalı.; 0000-0001-9836-0749; V-5578-2017; 36437200800; 35944810500The present study evaluated the changes in salivary proteome in parvoviral enteritis (PVE) in dogs through a high-throughput quantitative proteomic analysis. Saliva samples from healthy dogs and dogs with severe parvovirosis that survived or perished due to the disease were analysed and compared by Tandem Mass Tags (TMT) analysis. Proteomic analysis quantified 1516 peptides, and 287 (corresponding to 190 proteins) showed significantly different abundances between studied groups. Ten proteins were observed to change significantly between dogs that survived or perished due to PVE. Bioinformatics' analysis revealed that saliva reflects the involvement of different pathways in PVE such as catalytic activity and binding, and indicates antimicrobial humoral response as a pathway with a major role in the development of the disease. These results indicate that saliva proteins reflect physiopathological changes that occur in PVE and could be a potential source of biomarkers for this disease.Item Changes in serum proteins after endotoxin administration in healthy and choline-treated calves(BMC, 2016-09-10) İnan, Oya Eralp; Baykal, Ahmet T.; Hacariz, O.; Hatipoğlu, I.; Tvarijonaviciute, Asta; Ceron, Jose; Ulus, İsmail Hakkı; Yılmaz, Zeki; Cansev, Mehmet; Kocatürk, Meriç; Uludağ Üniversitesi/Veteriner Fakültesi/Dahiliye Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Eczacılık Anabilim Dalı.; 0000-0001-9836-0749; 0000-0003-2918-5064; M-9071-2019; V-5578-2017; 35944810500; 36437200800; 8872816100Background: This study aimed to investigate the possible serum protein changes after endotoxin administration in healthy and choline-treated calves using proteomics. These results are expected to contribute to the understanding of the pathophysiological mechanisms of endotoxemia and the beneficial effect of choline administration in this clinical situation. Methods: Healthy-calves (n = 20) were divided into 4 groups: Control, Choline treated (C), Lipopolysaccharide administered (LPS), and LPS + C. Control calves received 0.9 % NaCl injection. Calves in C and LPS + C groups received choline chloride (1 mg/kg/iv). Endotoxin (LPS) was injected (2 mu g/kg/iv) to the calves in LPS and LPS + C groups. Serum samples were collected before and after the treatments. Differentially expressed proteins (> 1.5 fold-change relative to controls) were identified by LC-MS/MS. Results: After LPS administration, 14 proteins increased, and 13 proteins decreased within 48 h as compared to controls. In the LPS group, there were significant increases in serum levels of ragulator complex protein (189-fold) and galectin-3-binding protein (10-fold), but transcription factor MafF and corticosteroid binding globulin were down regulated (>= 5 fold). As compared with the LPS group, in LPS + C group, fibrinogen gamma-B-chain and antithrombin were up-regulated, while hemopexin and histone H4 were down-regulated. Choline treatment attenuated actin alpha cardiac muscle-1 overexpression after LPS. Conclusions: LPS administration produces changes in serum proteins associated with lipid metabolism, immune and inflammatory response, protein binding/transport, cell adhesion, venous thrombosis, cardiac contractility and blood coagulation. The administration of choline is associated with changes in proteins which can be related with its beneficial effect in this clinical situation.Item Changes in serum proteins in dogs with Ehrlichia canis infection(Elsevier, 2017-10-13) Escribano, Damian; Martinez, Silvia Subiela; Ceron, Jose Joaquin; Tvarijonaviciute, Asta; Cihan, Hüseyin; Levent, Pınar; Kocatürk, Meriç; Aytuğ, Nilüfer; Yılmaz, Zeki; Uludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı.; 0000-0001-9836-0749; ABH-3742-2020; V-5578-2017; 6602381681; 56690616700; 36437200800; 6505835923; 35944810500The aim of this study was the identification of proteins differentially represented in the serum proteome of seropositive dogs with (Group 1) and without (Group 2) clinical-pathologic signs consistent with ehrlichiosis compared to healthy control dogs. Serum samples were collected from 20 dogs of various breeds with naturally occurring ehrlichiosis (10 dogs belonged to Group 1 and 10 to Group 2) and 10 healthy dogs. Two-dimensional electrophoresis (2DE) of pooled serum for each of the group of dogs were run in triplicate. 2D image analysis showed 39 spots differently expressed between Group 1 and Group 2 compared with healthy ones. Mass spectrometry analysis allowed identification of 6 proteins: albumin, haptoglobin (Hp), alpha-l-antitrypsin (AAT), Retinol Binding Protein 4 (RBP-4), alpha-l-acid glycoprotein (AGP) and vitamin D-binding protein (VDBP). When a confirmatory study was performed for albumin, Hp, AAT and RBP-4 by using different assays, significant differences (P < 0.05) between diseased and healthy groups were observed. It can be concluded that there are significant changes in the serum proteome of dogs with ehrlichiosis with modifications in proteins related with the acute phase response such as Hp, albumin and AGP, with vitamin A transport such as RBP-4, with inhibitors of serine proteases and anti-inflammatory proteins such as AAT, and vitamin D metabolism and actin scavengers such as VDBP.Item Effects of choline treatment in concentrations of serum matrix metalloproteinases (MMPs), MMP tissue inhibitors (TIMPs) and immunoglobulins in an experimental model of canine sepsis(Elsevier, 2016-08-17) Eralp, Oya İnan; Tvarijonaviciute, Asta; Ceron, J. Jose; Kocatürk, Meriç; Cansev, Mehmet; Özyiğit, Musa Özgür; Yılmaz, Zeki; Kahraman, Müjdat Müfit; Uludağ Üniversitesi/Veteriner Fakültesi/Dahiliye Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; 0000-0001-9836-0749; 0000-0003-2918-5064; AAH-2873-2021; AAR-6478-2021; A-9637-2008; M-9071-2019; V-5578-2017; 36437200800; 8872816100; 6507338060; 35944810500; 6701778876The aim of the present study was to investigate effects of intravenous (i.v.) choline treatment on serum matrix metalloproteinases (MMP), MMP tissue inhibitors (TIMP) and immunoglobulins (Igs), and to determine if there were relations between serum MMPs/TIMPs and C-reactive protein (CRP) (as a marker of the acute phase response), immunoglobulin G and M (IgG and IgM) (as a maker of the Ig responses) and markers of organ damage such as muscular damage (creatine phosphokinase, [CPK]), liver damage (alanine aminotransferase [ALT]) and renal dysfunction (blood urea nitrogen [BUN] and creatinine, [Cr]) in dogs with endotoxemia. Healthy dogs (n = 24) were randomized to Saline, Choline (C), Lipopolysaccharide (LPS), and LPS + C groups and received 0.9% NaCl (5 mL/i.v.), choline chloride (20 mg/kg/i.v.), LPS (0.02 mg/kg/i.v.) and LPS (0.02 mg/kg/i.v.) plus choline chloride (20 mg/kg/i.v.), respectively. Serum MMPs and TIMPs concentrations were analyzed by commercial ELISA kits. MMP and TIMP increased at 1-48 h (P < 0.05), whereas IgG and IgM decreased at 24-48 h in LPS group, compared to their baselines. Choline treatment reduced changes in serum MMPs, TIMPs and markers of organ damage, and prevented the hypoimmunoglobulinemia in LPS + C. MMPs and TIMPs were correlated positively (P < 0.05) with serum CRP, CPK, ALT, BUN and Cr, but not with serum Igs. Our findings suggest that the serum MMPs, TIMPs and Igs are involved in the pathophysiology of endotoxemia, and MMPs and TIMPs are correlated with the acute phase reaction and multi-organ failure. In addition, we demonstrated a direct effect of choline administration in decreasing serum MMPs and TIMPs, and preserving serum Igs in the course of endotoxemia.Item Prognostic value of serum acute-phase proteins in dogs with parvoviral enteritis(Wiley, 2010-09) Martinez, Silvia; Tvarijonaviciute, Asta; Ceron, Jose Joaquin; Kocatürk, Meriç; Eralp, Oya; Yılmaz, Zeki; Uludağ Üniversitesi/Veterinerlik Fakültesi/Klinik Bilimler Bölümü.; 0000-0001-9836-0749; 36437200800; 24472964600; 35944810500OBJECTIVE: To evaluate the acute-phase protein response in dogs with parvoviral enteritis as predictor of the clinical outcome. Methods: Canine parvovirus infection was diagnosed based on the compatible clinical findings and confirmed by the canine parvovirus antigen test in 43 dogs of less than six months of age. Blood samples for complete blood cell count and acute-phase proteins (C-reactive protein, haptoglobin, ceruloplasmin and albumin) were collected before treatment. Twenty-three dogs died during or after treatment (non-survival) and the rest recovered (survival). Five healthy dogs were enrolled as control. Results: Serum C-reactive protein, ceruloplasmin and haptoglobin levels in dogs with parvoviral enteritis were higher (P < 0.001, P < 0.01 and P < 0.001, respectively), but serum albumin was lower (P < 0.001) than those in controls. Mean C-reactive protein and ceruloplasmin values in non-survival were higher (P < 0.01) than those for survival dogs. C-reactive protein was found to be superior to ceruloplasmin, haptoglobin and albumin for distinguishing survival from non-survival dogs. Values higher than 92.4 mg/l for C-reactive protein had a sensitivity of 91% to predict mortality. Clinical Significance: The magnitude of the increase in serum acute-phase proteins in dogs with parvoviral enteritis could be a useful indicator of the prognosis of the disease. In acute-phase proteins, C-reactive protein is a potent predictor of mortality in dogs with parvoviral enteritis.Item Serum antioxidant capacity and oxidative damage in clinical and subclinical canine ehrlichiosis(Elsevier, 2017-06-10) Rubio, Camila Peres; Martínez, Silvia Subiela; Hernández, Josefa Ruiz; Tvarijonaviciute, Asta; Escribano, Damián; Ceron, Jose Joaquín; Yılmaz, Zeki; Kocatürk, Meriç; Yalçın, Ebru; Uludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı.; 0000-0001-9836-0749; V-5578-2017; 35944810500; 36437200800; 36778554000The objective of this study was to evaluate and compare the antioxidant response and the products of oxidative damage analysed by various assays in clinical and subclinical canine monocytic ehrlichiosis (CME). For this purpose, four assays to measure the total serum antioxidant capacity (TAC), such as the cupric reducing antioxidant capacity (CUPRAC), ferric reducing ability of plasma (FRAP), trolox equivalent antioxidant capacity (TEAC) using acidic medium (TEAC(A)), and the TEAC using the horseradish peroxidase (TEAC(H)) were used. In addition, the serum thiol concentrations were analysed. Reactive oxygen species (ROS), thiobarbituric acid reactive substances (TBARS), and ferrous oxidation-xylenol orange (FOX) were measured to determine the concentrations of free radical and the products of oxidative damage as result of the disease. All antioxidant markers were significantly lower in the dogs on clinical ehrlichiosis when compared with healthy dogs; however only the CUPRAC, FRAP and thiol were significantly lower in subclinical CME compared with healthy dogs. TBARS and FOX showed no significant differences between dogs with CME and healthy dogs; however, a significant increased ROS concentration was observed in dogs with clinical and subclinical CME when compared with healthy dogs. Results showed that in CME there is a state of oxidative stress with significant changes in markers of antioxidant defence and in concentrations of free radicals. However, the detection of these changes would depend of the assay used.Item Serum apolipoprotein-A1 as a possible biomarker for monitoring treatment of canine leishmaniosis(Elsevier, 2016-10-10) Escribano, Damian; Tvarijonaviciute, Asta; Joaquin Ceron, Jose; Pardo-Marin, Luis; Torrecillas, Alejandro; Martinez-Subiela, Silvia; Kocatürk, Meriç; Yılmaz, Zeki; Uludağ Üniversitesi/Veteriner Fakültesi/Dahiliye Anabilim Dalı.; 0000-0001-9836-0749; V-5578-2017; 36437200800; 35944810500The aims of this study were: the identification of proteins differentially represented in the serum proteome of dogs with leishmaniosis after treatment and the verification of one selected protein as a possible biomarker for treatment monitoring. Serum samples from five dogs with leishmaniosis, before and after treatment were pooled into two groups and analysed using 2-dimensional electrophoresis followed by mass spectrometry analysis (MS). The MS analysis allowed the identification of 8 proteins differently expressed. APO-A1 was selected and an immunoturbidimetric assay was validated for its measurement in dogs. Significantly decreased concentrations of APO-A1 in dogs with leishmaniosis and a significant increase after a good response to the treatment were observed, suggesting that APO-A1 could be a potential biomarker of treatment monitoring with the advantages of an automated measurement.Item Serum butyrylcholinesterase and paraoxonase 1 in a canine model of endotoxemia: Effects of choline administration(Elsevier Science, 2012-10) Tvarijonaviciute, Asta; Tecles, Fernando; Ceron, Jose J.; Kocatürk, Meriç; Cansev, Mehmet; Yılmaz, Zeki; Uludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji Anabilim Dalı.; 0000-0001-9836-0749; M-9071-2019; 36437200800; 8872816100; 35944810500Butyrylcholinesterase (BChE) and paraoxonase 1 (PON1) are two serum enzymes synthesized by the liver that are related with inflammation. The main objectives of this study were to determine changes in serum BChE and PON1 by using a canine model of endotoxemia, and to evaluate whether choline alters BChE and PON1 activities during inflammation. For this purpose, a total of 20 mongrel dogs were divided into four groups: control, choline (C), lipopolysaccharide (LPS), and LPS + C. Dogs in the control group were injected with 0.9% NaCl (0.2 ml/kg, i.v.). Dogs in C and LPS + C groups received choline chloride (20 mg/kg, i.v., three times with 4 h intervals). Endotoxin was injected (0.02 mg/kg, i.v., once) to the dogs of LPS and LPS + C groups. Statistically significant decreases in BChE and PON1 activities in LPS group were detected 24 and 48 h post injection, respectively. No statistically significant changes in BChE and PON1 activities at different times were detected in control, C, or LPS + C groups. In conclusion, the data obtained in present study revealed a decrease in serum BChE and PON1 activities in dogs during experimentally induced endotoxemia and that choline administration attenuates these changes.Item Serum choline and butyrylcholinesterase changes in response to endotoxin in calves receiving intravenous choline administration(Elsevier, 2019-07-09) Eralp İnan, Oya; Tvarijonaviciute, Asta; Rubio, Camila Peres; Cerón, José Joaquín; Ulus, Ismail Hakki; Kocatürk, Meriç; Kasap, Sevim C.; Cansev, Mehmet; Yılmaz, Zeki; Uludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı; Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji Anabilim Dalı.; 0000-0002-2849-1222; 0000-0001-9836-0749; V-5578-2017; AAP-7998-2020; M-9071-2019; A-9637-2008; 36437200800; 36514844800; 8872816100; 35944810500Endotoxemia treatment options are still of interest due to high mortality and choline treatment is one of them because of its role in the cholinergic anti-inflammatory pathway. This study investigated serum choline and butyrylcholinesterase (BChE) responses, and their correlations with inflammatory, oxidative stress and tissue damage biomarkers, including paraoxanase-1 (PON1), and clinical signs in calves with endotoxemia and the effect of choline treatment in these responses. Healthy calves (n = 20) were divided equally into 4 groups: Control (0.9% NaCl, iv), Choline (C; 1 mg/kg/iv,once), Lipopolysaccharide (LPS; 2 mu g/kg/iv,once) and LPS + C. Clinical and laboratory examinations were performed before and 0.5-48 h (hrs) after treatments. Following LPS administration, serum choline level increased at 0.5-24 h (P < .01), whereas serum BChE and PON1 level decreased at 48 h (P < .01) compared to their baselines. In LPS + C group, the increase in serum choline level was significantly higher (P < .01) than that of C and LPS groups. LPS did not decrease serum BChE levels significantly in calves treated with choline. Serum choline and BChE results correlated negatively with white blood cell count and positively (P < .001) with PON1 levels, oxidative stress index, inflammation and hepato-muscular injury markers. In conclusion serum choline and BChE may have a role in the pathophysiology of endotoxemia in calves. High serum choline concentration is associated with an improvement in response to LPS administration in calves treated with choline, probably by preventing the imbalances between oxidative stress and anti-oxidant capacity, preventing the serum BChE and PON1 decreases, and inhibition/attenuation of acute phase reaction and hepato-muscular injury in calves with endotoxemia.Item Tei index (myocardial performance index) and cardiac biomarkers in dogs with parvoviral enteritis(Elsevier, 2012-02) Martinez, Silvia; Tvarijonaviciute, Asta; Ceron, Jose; Kocatürk, Meriç; Eralp, Oya; Yılmaz, Zeki; Uludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı.; 0000-0002-4242-8609; 0000-0001-9836-0749; AAG-2943-2020; 36437200800; 24472964600; 35944810500Tei index (myocardial performance) and cardiac biomarkers were evaluated in dogs with parvoviral enteritis (PVE). Tei index was calculated as isovolumic contraction time plus isovolumic relaxation time divided by ejection time. Myocardial and skeletal muscle damages were assessed by serum levels of cardiac troponin I (cTnI), creatine (phospho) kinase, lactate dehydrogenase and aspartate aminotransferase. Serum magnesium level was also determined. According to treatment response, dogs were divided into the survivor (n = 20) and non-survivor groups (n = 23). Seven healthy dogs served as controls. The mean value of the Tei index was higher in non-survivors, compared with survivors (p < 0.02) and healthy controls (p < 0.01). Serum level of cTnI in non-survivors was higher than that of survivors and controls (p < 0.05). Tei index showed the highest sensitivity and specificity to predict mortality. The findings of an elevated Tei index and an increase in serum cTnI are factors associated with a poor prognosis in cases of canine parvovirosis.