Browsing by Author "Wehrend, Axel"
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Item Clinical case: Unilateral en bloc ovariocornuectomy as a treatment for uterine torsion in a bitch(Ecole National Veterinaire Toulouse, 2011-02) Wehrend, Axel; Kumru, İsmail Hakkı; İntaş, Kamil Seyrek; İntaş, Deniz Seyrek; Tek, Hasan Basri; Uludağ Üniversitesi/Veterinerlik Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; Uludağ Üniversitesi/Veterinerlik Fakültesi/Cerrahi Anabilim Dalı.; AAH-7292-2019; 16637505700; 6603409870; 6506990178; 6603546035Uterine torsion is an uncommon, but life-threatening condition in the bitch and is routinely treated by ovariohysterectomy that means the end of breeding life. This report describes unilateral en bloc ovariocornuectomy as a successful treatment for uterine torsion in a heavily pregnant bitch by preserving the fertility including long term follow-up. A torsion-induced unilateral uterine necrosis was observed at laparatomy. At surgery two dead fetuses were retrieved by unilateral en bloc ovariocornuectomy. One week postoperatively, the bitch had fully recovered without problem. In the following breeding periods the bitch gave birth twice to two and three healthy puppies, respectively. Bitches with dystocia due to torsion of one uterine horn complicated with necrosis may survive if immediate unilateral en bloc ovariocornuectomy is performed while breeding ability of the bitch is preserved.Item Effects of the progesterone receptor antagonist aglepristone on implantation administered on days 6 and 7 after mating in rabbits(Wiley, 2010-06) Wehrend, Axel; Özalp, Gözde Rabia; Çalışkan, Çağlar; Seyrek İntaş, Kamil; Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; 0000-0003-4694-6937; AAE-3607-2019; AAH-7292-2019; 23985710500; 23984353800; 6603409870Contents There is no safe and accurate method for early termination of pregnancy in the rabbit. So this study was carried out to determine the effect of aglepristone administration in preventing early pregnancy before implantation in this species. Twenty-two animals (10-12 months old, New Zealand White rabbits) were naturally mated and pregnancies were confirmed in all animals by ultrasonographic examinations on day 6 after mating (5-7.5 MHz linear array transducer Dynamic Imaging Sonostar, UK) and the animals were grouped randomly: Group I & Group III: Aglepristone (Alizin (R), Virbac; 10 mg/kg, subcutaneously) was injected twice, 24 h apart, on days 6 and 7 after mating (n = 5; n = 8). Group II & Group IV: The same volume of 0.9% NaCl solution was subcutaneously injected in the same interval and served as controls (n = 5; n = 3). Ultrasonographical examination of the uterus was performed daily from day 7 to day 11 post-mating to test aglepristone efficiency. Blood samples were collected between days 6 and 30, centrifuged at 3070 g for 10 min and stored at -20 degrees C. The does in aglepristone groups (Group I, III) were not pregnant whereas all animals in control groups were pregnant (Group II, IV). The does in group I & III were examined only clinically and ultrasonographically; however, does in groups III and IV were laparomized on days 6, 7, 9 and 11 post-mating to control countable implantation sites. No implantation sites were present in group III whereas they were seen obviously in group IV. Side effects were not observed. The mean serum progesterone (P4) concentrations were not significantly different between control and treated does (p > 0.05). The results indicate that aglepristone treatment on days 6 and 7 after mating could prevent pregnancy after unwanted matings without any side effects in the rabbit. Aglepristone treatments are possibly not affecting further fertilities before implantation.Publication Genotoxic and cytotoxic effects of the aglepristone, a progesteron antagonist, in mid-gestation pregnancy termination in rabbits(Kafkas Univ, Veteriner Fakultesi Dergisi, 2015-03-01) Vatan, Özgür; Bağdaş, Deniz; Çinkılıç, Nilüfer; Wehrend, Axel; Özalp, Gözde Rabia; VATAN, ÖZGÜR; Bagdas, Deniz; ÇİNKILIÇ, NİLÜFER; ÖZALP, RABİA GÖZDE; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.; Uludağ Üniversitesi/Tıp Fakültesi/Deney Hayvanları Yetiştirme ve Araştırma Merkezi.; Uludağ Üniversitesi/Veteriner Fakültesi/Jinekoloji Anabilim Dalı.; 0000-0002-7687-3284; 0000-0002-3595-6286; 0000-0003-4694-6937; ISV-0209-2023; O-7508-2015; AAH-5296-2021; AAE-3607-2019; EOB-5882-2022Aglepristone is an antiprogestin using for pregnancy termination in veterinary medicine. The information about side effects of aglepristone is limited. The aim of the study was to investigate cytotoxicity and genotoxicity of aglepristone in mid-gestation pregnancy termination in rabbits. Fifteen New Zealand White rabbits were used and pregnant does were randomly divided into three groups. Group I (n=5) was treated with saline as the control. The does in group II (n=5) and group III (n=5) were treated with aglepristone (10 mg/kg) on 15th day and 15th-16th days of pregnancy, respectively. The rabbits were sacrificed by guillotine 24 h after last treatment. Bone marrow and blood samples were immediately collected. Cytotoxic and genotoxic potential were tested by micronucleus and Comet assays. No genotoxicity and cytotoxicity were found in micronucleus test with single aglepristone administration. In contrast, two consecutive treatments of aglepristone showed high genotoxic and cytotoxic effects on bone marrow in animals. While comet assay of blood samples did not show any significant difference between groups; the results from comet assay of bone marrow cells showed the single injection of aglepristone did not induce any DNA damage but two injections group increased the DNA damage.Item Incidence of uterine torsion as a cause of dystocia in bitches(Wiley, 2009-02) Walter, Yvonne; Wehrend, Axel; Kumru, İ. H.; İntaş, Kamil Seyrek; İntaş, Deniz Seyrek; Uludağ Üniversitesi/Veterinerlik Fakültesi/Doğum ve Jinekoloji Anabilim Dalı.; AAH-7292-2019Item Mid-gestation pregnancy termination in rabbits by the progesterone antagonist aglepristone(Elsevier, 2008-06) Wehrend, Axel; Seyrek-İntaş, Kamil; Çalişkan, Çaǧlar; Özalp, Gözde Rabia; Uludağ Üniversitesi/Veteriner Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; 0000-0003-4694-6937; AAE-3607-2019; AAH-7292-2019; 23985710500; 6603409870; 23984353800The efficacy of aglepristone treatment to induce abortion in does 15 and 16 days after mating was investigated. The pregnant does were randomly allocated into two groups: For group I, aglepristone was injected twice (10 mg/kg, subcutaneously) on days 15 and 16 after mating (n = 10); for group II the does got no treatment but the same volume of 0.9% sodium chloride solution was subcutaneously injected at the same days of pregnancy (n = 5). Results: group I, termination of pregnancy was successful in all does. The mean interval between the first administration of aglepristone and the beginning of vaginal discharge was 32.4 ± 5.6 h (range 19-72 h). Complete expulsion of all fetuses was observed in four does with first occurrences of vaginal discharge on the same day. The duration between the first occurrence of vaginal discharge to expulsion of all fetuses ranged between 21 and 130 h (mean 70.2 ± 12.2 h). As important side effects, decrease in food consumption during abortion time and irregular mating behaviour (52.3 ± 2.0 days/range 46-63) were recorded. But after this time all does were mated again, 8/10 became pregnant and they whelped normal and live kittens. Group II, all does gave birth to live kittens after a mean pregnancy length of 31.2 ± 0.37 days (range 30-32 days). The mean serum progesterone (P4) concentrations were significantly different between control and treated does after day 20 of pregnancy (P < 0.05). The results indicate that aglepristone treatment is effective to induce abortion in does and causes no serious negative effects on further fertility except a short non-receptive period after abortion and short time decrease in food consumption.Item Parturition induction in ewes by a progesterone receptor blocker, aglepristone, and subsequent neonatal survival: Preliminary results(Elsevier, 2016-08-14) Şeker, İbrahim; Rişvanlı, Ali; Demiral, Ömer Orkun; Wehrend, Axel; Özalp, R. Gözde; Yavuz, A.; Orman, Abdülkadir; Udum, Duygu Küçükşen; Uludağ Üniversitesi/Veteriner Fakültesi.; Uludağ Üniversitesi/Veteriner Fakültesi/Zootekni Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Biyokimya Anabilim Dalı.; 0000-0003-4694-6937; 0000-0001-9138-4422; AAE-3607-2019; AAG-9134-2021; 23985781000; 57191907999; 24335834100The clinical effects of aglepristone treatment to induce parturition in ewes and their newborns were reported. Three experimental groups were defined: group AG5 (n = 5), group AG10 (n = 5), and group CG (n = 5) in which ewes were injected twice with 5, 10 mg/kg of aglepristone, and saline solution of ewes, respectively. Different parameters associated with parturition in ewes and their newborns were investigated. Serum progesterone, oxytocin, and free and conjugated total estrogens were measured after treatments until parturition. No statistical difference was found from first aglepristone administration to onset of lambing between AG5 and AG10 (23.90 +/- 6.20, 40.00 +/- 6.71 hours). Parturition induction in two groups shortened the gestational length significantly compared with the control group (P = 0.003). Dystocia was observed in two ewes in group AG10. The placental weight showed statistically significant difference only between the AG10 and CG (P = 0.039), but no difference was observed in the placental expulsion period between the groups. Decrease in food consumption 24 to 36 hours after parturition in all ewes and skin necrosis in an ewe in group AG5 were observed. Progesterone concentration was significantly lower in AG5 than that in ewes in group AG10 and CG (P < 0.05). No difference was observed in concentrations of free total estrogens and oxytocin between groups. The body temperature of lambs was significantly different between AG10 and CG groups both right after (P = 0.011) and 12 hours after parturition (P = 0.014). The lambs in CG had the highest mean birth weight (4.29 +/- 0.28 kg), which was significantly different from the induced groups. No significant difference of blood pH and blood gases values between groups was identified both at birth and 12 hours after parturition for lambs. Significant differences could clearly be observed in total protein and blood urea nitrogen and total protein findings 12 hours after parturition (P < 0.05), whereas no difference was found in blood glucose, albumin, inorganic phosphor, triglyceride, or total cholesterol parameters. The results of this study show that the administration of aglepristone to induce parturition can precisely control lambing time without any side effects in either mothers or lambs.Item Unilateral hysterectomy (cornuectomy) in the bitch and its effect on subsequent fertility(Elsevier, 2004-06) Wehrend, Axel; Gökhan, Turhan; Bostedt, Hartwig; İntaş, Kamil Seyrek; Nak, Yavuz; Tek, Hasan Basri; Yılmazbaş, Gülnaz; Uludağ Üniversitesi/Veteriner Fakültesi/Doğum ve Ji̇nekoloji̇ Anabi̇li̇m Dalı.; 0000-0003-2817-3221; 0000-0003-2999-3491; AAH-7292-2019; AAH-5494-2021; 6603409870; 8615464000; 6603546035; 36457964000During caesarean section of bitches a beginning tissue necrosis of the uterus is often encounted. These alterations mostly require ovariohysterectomy that means the end of breeding life. The aim of this study was to create a model for unilateral hysterectomy during dystocia and to evaluate subsequent fertility. Unilateral cornuectomy was performed in 18 clinically healthy bitches of different ages, breeds, and at different stages of the sexual cyclus. Four bitches were not available for follow-up examinations. Twelve bitches were mated at the first obvious estrus period postoperatively and 10 pregnancies were diagnosed. Nine bitches delivered one to five puppies (mean 3.8) after a gestation period of 63-67 days. The puppies (n = 38) were in a very good condition and showed high vitality. Unilateral cornuectomy of the uterus had no adverse effects and postoperative mating revealed pregnancy without complications and normal parturition. In the case of pathological changes in one uterine horn during a caesarean section unilateral hysterectomy seems to be an alternative to ovariohysterectomy.Item Utero-ovarian torsion in a bitch and subsequent fertility after unilateral ovariohysterectomy(George Thieme, 2011) Wehrend, Axel; Salcı, Hakan; İntaş, Deniz Seyrek; Kumru, İsmail Hakkı; İntaş, Kamil Seyrek; Uludağ Üniversitesi/Veterinerlik Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; Uludağ Üniversitesi/Veterinerlik Fakültesi/Cerrahi Anabilim Dalı.; AAH-7292-2019; T-4623-2019; 8680329000; 6506990178; 16637505700; 6603409870