Browsing by Author "YAVAŞ, ÖZKAN"
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Publication Comparison of cytological, microbiological and histopathological findings of genital tracts in cows with different degree perineal conformation disorder(Sivar-soc Italiana Veterinari Animali Reddito, 2021-02-01) Intas, Kamil Seyrek; Salci, Emsal Sinem Ozdemir; ÖZDEMİR SALCI, EMSAL SİNEM; Yavas, Ozkan; YAVAŞ, ÖZKAN; Ardicli, Ozge Yilmaz; Sonmez, Gursel; SÖNMEZ, GÜRSEL; Demirbilek, Serpil Kahya; KAHYA DEMİRBİLEK, SERPİL; Ardicli, Sena; ARDIÇLI, SENA; Bursa Uludağ Üniversitesi/Veteriner Fakültesi.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı.; 0000-0001-6077-0478; 0000-0003-2758-5945; KHD-4075-2024; O-3394-2019; AAG-7421-2021; AAH-5294-2021The aim of this study is to investigate cytological, microbiological and histopathological findings of the genital tracts in cows with different degree perineal conformation (PC) disorder. Totally, 28 cows brought the slaughterhouse were materials of the study. Information about to age, body weight, reproductive status, number of parturitions, days in milk (DIM) and the reason for slaughtering of the cows was obtained in the anamnesis learned from the owners. Before slaughtering, cows were evaluated in terms of PC and then they were divided into four groups with equal numbers of cows according to PC disorders: group I (GRI) normal; group II (GRII) mild; group III (GRIII) moderate and group IV (GRIV) severe. PC disorders of the cows were graded on a scale in terms of vulvar angle, vulvar length over ischial arch, depth of anus and perineal length. A body condition score (BCS) was also evaluated in the cows. The perineal region and vagina were inspected for vaginal discharge and vaginal mucosal appearance, respectively. Pneumovagina was classified as negative, suspicious and positive. Sterile swab samples were taken from vagina, cervix and uterus for bacteriological culture and identification. For cytological examination, smears were taken from vagina, cervix and uterus. Tissue samples were taken from vagina, cervix and uterus for histopathological examination. Statistically, Pearson's correlation, Fisher's exact tests and regression analysis were performed for all data. Clinically, characters of the vaginal discharge (serous, foamy, mucous, purulent and urine-mixed) and vaginal hyperemia increased in GRIII and GRIV. Microbiologically, as parallel to the PC disorder, E. coli in Enterobacteriaceae family was the most common bacterium in Group III and Group IV. As PC disorder in the groups increased, cytological examination findings were found to be significant, similar to clinical and microbiological examination. However, histopathological examination gave more meaningful results in groups. Statistic results pointed out that difference between the groups in terms of vaginal mucosa color, cervical and uterine microbiology was significant. In conclusion, PC disorder in cows constitutes predisposition for many genital canal diseases that may be the cause of infertility. The presence of genital canal diseases can be suspected in cattle using the PC scale. Therefore, it is appropriate to evaluate the health status of the genital canal with other diagnostic methods (microbiology, cytology and histopathology), especially in cows with PC disorder.Publication Description of lens atrophy caused by Shewanella sp. strain S-1, a putative novel species isolated from cultured rainbow trout(Elsevier, 2021-04-13) Satıcıoğlu, İzzet Burçin; İsbilir, Fatma; Yavaş, Özkan; Avcı, Zehra; Özdemir, Burak; Süzer, Bayram; Altun, Soner; Duman, Muhammed; İŞBİLİR, FATMA; YAVAŞ, ÖZKAN; Avcı, Zehra; SÜZER, BAYRAM; ALTUN, SONER; DUMAN, MUHAMMED; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Anatomi Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Su Hayvanları Hastalıkları Anabilim Dalı.; 0000-0002-2687-1221; 0000-0001-7707-2705; 0000-0001-9096-875X; HJI-5667-2023; KHD-4075-2024; X-2000-2019; GLV-3407-2022; T-1697-2019; EZZ-5951-2022; CCJ-5608-2022; AAG-8518-2021In the present study, we describe a pathogenic incidence of Shewanella sp. S-1 infection in cultured rainbow trout (Oncorhynchus mykiss, Walbaum, 1792), as evidenced by lens atrophy, inappetence, visual impairment, and growth retardation. Strain S-1 was identified using basic microbiological tests, the BD PhoenixTM rapid diagnostic kit, the MALDI-TOF MS system, and whole-genome analysis. This strain's pathogenicity was assessed based on the histopathological examination of the eyes, lens, muscle, brain, liver, kidney, spleen, and gills. The lens, brain, and eyes of the fish were also examined morphologically. According to genome-based delineation, strain S-1 was determined to belong to the genus Shewanella and was considered a putative novel Shewanella species. While we determined that the lens had atrophied at a level ranging from 30% to 40%, no severe lesion was detected in other organs. Fish that hatched in the same period were bred under uniform feeding and management conditions for a month. Monitoring data revealed that, at the end of this 1-month period, the growth rate and feed conversion rate of the diseased fish were significantly lower than those of the healthy group. Based on microbiological and pathological findings, we concluded that Shewanella sp. S-1 caused lens atrophy in the rainbow trout.Publication Expression profile of prostaglandin enzymes in cystic endometrial hyperplasia in dogs: The results of a hypothesis in clinical trial(Kafkas Üniversitesi, 2023-01-30) Korlu, Yeşim; Yavaş, Özkan; Aktar, Ahmet; Bozkurt, Berkay; Özyiğit, Musa Özgür; Özalp, Gözde Rabia; Korlu, Yeşim; YAVAŞ, ÖZKAN; Aktar, Ahmet; ÖZYİĞİT, MUSA ÖZGÜR; ÖZALP, RABİA GÖZDE; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Jinekoloji ve Doğum Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Döreme ve Suni Tohumlama Anabilim Dalı.; 0000-0002-2975-2594; AAE-3607-2019; KHD-4075-2024; CZR-5232-2022; CBC-1744-2022; HPY-3936-2023The expressions of prostaglandin synthesis enzymes and estrogen, progesterone receptors in canine cystic endometrial hyperplasia (CEH) were reported in this manuscript. Uterine tissue samples were collected from bitches with CEH (n=5), CEH-P (Cystic endometrial hyperplasia-Pyometra) (n=5) and healthy-negative control group, CG (n=5). Immunohistochemistry was carried out for the estrogen (ER) and progesterone receptor (PR) detection. Shock-frozen samples were utilized in mRNA extraction and Real-Time PCR was performed. Gene expression of PTGS2/COX2, PTGES, PTGER4, PGFS, PTGFR and PGR were detected higher in the CEH group compared with CG. The PGFS and PTGFR (FP) mRNA expressions were significantly increased in CEH compared with other groups. Expression of progesterone receptor mRNA (PGR) was highest in CEH and statistically different from the CEH-P group (P<0.05). No PR immunostaining was observed. ER staining had been detected in endometrial glands, endometrial stoma and myometrium, however hyperplasic glands in propria mucosa had lower or no ER scores. Based on the results of this study, the high levels of prostaglandin enzymes and low ER scores in CEH could be a preliminary step for the next stages of severe differentiation of endometrium.Publication Identification of mast cells and immunophenotypic subtypes in peripheral nerve damage caused by epineurotomy and systemic inflammation(Wolters Kluwer Medknow Publications, 2023-01-01) Yavaş, Özkan; YAVAŞ, ÖZKAN; Yavaş, Senem Esin; Kavruk, Kaan; KAVRUK, KAAN; Özbek, Serhat; ÖZBEK, SERHAT; Ersoy, Semiha; ERSOY, SEMİHA; Bursa Uludağ Üniversitesi/Veteriner Fakültesi; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Plastik Cerrahi Anabilim Dalı.; KHD-4075-2024Purpose: This study aims to investigate the numerical increase, localization, granulation status, and immunophenotypic properties of mast cells (MCs) in epineurectomy-induced nerve damage and lipopolysaccharide (LPS)-induced systemic infection models. Materials and Methods: In this study, the animals were divided into three groups of 6 each. One of the groups was determined as the control group, epineurectomy was applied to one group, and systemic inflammation was created by regular LPS injections in the other group. Then, the obtained nerve tissues were stained histochemically with Hematoxylin and Eosin toluidine blue, and the increase, localization, and granulation status of MCs were examined. Immunohistochemically, antitryptase and antichymase staining were performed to determine the immunophenotypes of MCs. Results: As a result, while the number of MCs increased in both groups compared to the control group, MCs in the LPS group were in the epineurium, and MCs in the epineurotomy group were located between the nerve fibers. While MCs in the LPS group showed very severe degranulation, mild degranulation was observed in the epineurotomy group, and almost no degranulated MCs were observed in the control group. Conclusion: This study is critical because it is one of the first studies to compare MCs in different nerve damage types and examine the expression of chymase and tryptase.