Browsing by Author "Yadollahi, Abbas"
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Item In vitro culture of gisela 6 semi-dwarf rootstock(Uludağ Üniversitesi, 2013-06-03) Aghaye, Rahim Nazary Moghaddam; Yadollahi, Abbas; Moeini, Ahmad; Sepahvand, SadeghThis research was carried out to study micropropagation of Gisela 6 rootstock in order to achieve suitable media for producing 5 thousand plants. Explants were cultured in MS and WPM media containing 2 types of cytokinins BAP and TDZ at different concentrations. The results showed that the number of shoots in MS medium containing 1.2 mg L BAP was higher than the other treatments. For rooting, the effect of basal media (MS, 1/2 MS (macroelements half) and 1/2 MS (macro and micro elements half)) and different auxins including IBA, NAA and IAA, solely or in combination with each other was investigated. The results showed that the highest percentage of rooting was due to medium 1/2 MS containing (macroelements half) 1 mg L -1 IBA resulted in 100% rooting. Due to low quality of roots obtained, and also the role of Fe-EDDHA and thiamine in rooting improvement, an experiment about the effects of above-mentioned elements was carried out. The results indicated that thiamine and Fe-EDDHA at concentrations of 1.6 and 150 mg L -1 resulted in the best rooting. Finally, in acclimation phase, more than %95 survival was achieved. -1 respectivelyItem In vitro culture of gisela 6 semi-dwarf rootstock(Uludağ Üniversitesi, 2013-06-03) Moghaddam, Rahim Nazary; Yadollahi, Abbas; Moeini, Ahmad; Sepahvand, SadeghThis research was carried out to study micropropagation of Gisela 6 rootstock in order to achieve suitable media for producing 5 thousand plants. Explants were cultured in MS and WPM media containing 2 types of cytokinins BAP and TDZ at different concentrations. The results showed that the number of shoots in MS medium containing 1.2 mg L-1 BAP was higher than the other treatments. For rooting, the effect of basal media (MS, 1/2 MS (macroelements half) and 1/2 MS (macro and micro elements half)) and different auxins including IBA, NAA and IAA, solely or in combination with each other was investigated. The results showed that the highest percentage of rooting was due to medium 1/2 MS containing (macroelements half) 1 mg L-1 IBA resulted in 100% rooting. Due to low quality of roots obtained, and also the role of Fe-EDDHA and thiamine in rooting improvement, an experiment about the effects of above-mentioned elements was carried out. The results indicated that thiamine and Fe-EDDHA at concentrations of 1.6 and 150 mg L-1 respectively resulted in the best rooting. Finally, in acclimation phase, more than %95 survival was achieved.Item In vitro screening of almond (Prunus dulcis (Mill.)) genotypes for drought tolerance(Uludağ Üniversitesi, 2012) Karimi, Soheil; Yadollahi, Abbas; Moghadam, Rahim Nazari; Imani, Ali; Arzani, KazemScreening and identifying drought tolerant almond genotypes is required in order to improve and stabilize almond production under semi-arid and arid conditions. In this study, we evaluated responses of five high yield and late blooming almond genotypes to drought stress induced in vitro, and compared their drought tolerance to the drought tolerant peach×almond hybrid ‘GF677’. Explants established on the MS medium and subjected to three polyethylene glycol (PEG) osmotic stress for 40 days. Plant growth indices, leaves relative water content (RWC), membrane stability index (MSI), were significantly reduced under drought stress. On the other hand, proline concentration and number of lateral shoots were significantly increased under drought stress. Drought tolerant genotypes maintained higher levels of RWC, and MSI under drought stress. Growth of drought tolerant cultivars was more stable. Proline accumulation in the explants was found to be a general response of almond to drought stress. The results suggested that drought tolerant almond genotypes may be screened by in vitro experiments.Item In vitro screening of almond (Prunus dulcis (Mill.)) genotypes for drought tolerance(Uludağ Üniversitesi, 2012-12-06) Karimi, Soheil; Yadollahi, Abbas; Moghadam, Rahim Nazari; Imani, Ali; Arzani, KazemScreening and identifying drought tolerant almond genotypes is required in order to improve and stabilize almond production under semi-arid and arid conditions. In this study, we evaluated responses of five high yield and late blooming almond genotypes to drought stress induced in vitro, and compared their drought tolerance to the drought tolerant peach×almond hybrid ‘GF677’. Explants established on the MS medium and subjected to three polyethylene glycol (PEG) osmotic stress for 40 days. Plant growth indices, leaves relative water content (RWC), membrane stability index (MSI), were significantly reduced under drought stress. On the other hand, proline concentration and number of lateral shoots were significantly increased under drought stress. Drought tolerant genotypes maintained higher levels of RWC, and MSI under drought stress. Growth of drought tolerant cultivars was more stable. Proline accumulation in the explants was found to be a general response of almond to drought stress. The results suggested that drought tolerant almond genotypes may be screened by in vitro experiments.