Browsing by Author "Yavaş, Özkan"

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Comparison of the effects of ketamine-diazepam, tiletamine-zolazepam and propofol infusion anesthesia in rabbits
(Kafkas Üniversitesi, 2023-02-14) Topal, Ayşe; Satar, Nihal Yaşar Gül; Ateş, Özge; Uçkan, Elyesa Melih; Yavaş, Özkan; Cangül, İbrahim Taci; TOPAL, AYŞE; GÜL SATAR, NİHAL YAŞAR; Ateş, Özge; Uçkan, Elyesa Melih; YAVAŞ, ÖZKAN; CANGÜL, İBRAHİM TACİ; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Cerrahi Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; KHD-4075-2024; AAB-4360-2021; GGO-6894-2022; FTA-8490-2022; CEC-2677-2022; HQA-9810-2023
In this study we evaluated the anesthetic effects of ketamine-diazepam (K-D), tiletamine zolazepam (T-Z), and propofol (P) infusion for one h in rabbits, and also the impact of these anesthetics on physiological and biochemical parameters, and the tissue structure of the liver, kidney, and lungs. 18 New Zealand white rabbits were allocated to three administration groups. All groups were premedicated with xylazine. For induction, ketamine and diazepam were administered intravenously in group K-D, tiletamine-zolazepam combination was administered in group T-Z, and propofol was administered in group P. After induction, infusion anesthesia was continued for one h by intravenously ketamine and diazepam in group K-D, tiletamine-zolazepam combination in group T-Z, and propofol in group P. The respiratory rate significantly decreased in the P group at 30, 45, and 60 min compared to the initial values (P<0.05). End-tidal CO2 significantly increased in the P group at 30, 45, and 60 min compared to the initial values (P<0.05). All physiological and serum biochemical parameters were within the reference ranges. Infusions resulted in varying degrees of degenerative changes in the kidney, lung, and liver, but these changes did not affect the anesthesia status or biochemical parameters. We suggest that K-D, T-Z, and P can be applied safely as an infusion for one h anesthesia of rabbits as an alternative to traditional anesthesia regimens.
Description of lens atrophy caused by Shewanella sp. strain S-1, a putative novel species isolated from cultured rainbow trout
(Elsevier, 2021-04-13) Satıcıoğlu, İzzet Burçin; İsbilir, Fatma; Yavaş, Özkan; Avcı, Zehra; Özdemir, Burak; Süzer, Bayram; Altun, Soner; Duman, Muhammed; İŞBİLİR, FATMA; YAVAŞ, ÖZKAN; Avcı, Zehra; SÜZER, BAYRAM; ALTUN, SONER; DUMAN, MUHAMMED; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Anatomi Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Su Hayvanları Hastalıkları Anabilim Dalı.; 0000-0002-2687-1221; 0000-0001-7707-2705; 0000-0001-9096-875X; HJI-5667-2023; KHD-4075-2024; X-2000-2019; GLV-3407-2022; T-1697-2019; EZZ-5951-2022; CCJ-5608-2022; AAG-8518-2021
In the present study, we describe a pathogenic incidence of Shewanella sp. S-1 infection in cultured rainbow trout (Oncorhynchus mykiss, Walbaum, 1792), as evidenced by lens atrophy, inappetence, visual impairment, and growth retardation. Strain S-1 was identified using basic microbiological tests, the BD PhoenixTM rapid diagnostic kit, the MALDI-TOF MS system, and whole-genome analysis. This strain's pathogenicity was assessed based on the histopathological examination of the eyes, lens, muscle, brain, liver, kidney, spleen, and gills. The lens, brain, and eyes of the fish were also examined morphologically. According to genome-based delineation, strain S-1 was determined to belong to the genus Shewanella and was considered a putative novel Shewanella species. While we determined that the lens had atrophied at a level ranging from 30% to 40%, no severe lesion was detected in other organs. Fish that hatched in the same period were bred under uniform feeding and management conditions for a month. Monitoring data revealed that, at the end of this 1-month period, the growth rate and feed conversion rate of the diseased fish were significantly lower than those of the healthy group. Based on microbiological and pathological findings, we concluded that Shewanella sp. S-1 caused lens atrophy in the rainbow trout.
Expression profile of prostaglandin enzymes in cystic endometrial hyperplasia in dogs: The results of a hypothesis in clinical trial
(Kafkas Üniversitesi, 2023-01-30) Korlu, Yeşim; Yavaş, Özkan; Aktar, Ahmet; Bozkurt, Berkay; Özyiğit, Musa Özgür; Özalp, Gözde Rabia; Korlu, Yeşim; YAVAŞ, ÖZKAN; Aktar, Ahmet; ÖZYİĞİT, MUSA ÖZGÜR; ÖZALP, RABİA GÖZDE; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Jinekoloji ve Doğum Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Döreme ve Suni Tohumlama Anabilim Dalı.; 0000-0002-2975-2594; AAE-3607-2019; KHD-4075-2024; CZR-5232-2022; CBC-1744-2022; HPY-3936-2023
The expressions of prostaglandin synthesis enzymes and estrogen, progesterone receptors in canine cystic endometrial hyperplasia (CEH) were reported in this manuscript. Uterine tissue samples were collected from bitches with CEH (n=5), CEH-P (Cystic endometrial hyperplasia-Pyometra) (n=5) and healthy-negative control group, CG (n=5). Immunohistochemistry was carried out for the estrogen (ER) and progesterone receptor (PR) detection. Shock-frozen samples were utilized in mRNA extraction and Real-Time PCR was performed. Gene expression of PTGS2/COX2, PTGES, PTGER4, PGFS, PTGFR and PGR were detected higher in the CEH group compared with CG. The PGFS and PTGFR (FP) mRNA expressions were significantly increased in CEH compared with other groups. Expression of progesterone receptor mRNA (PGR) was highest in CEH and statistically different from the CEH-P group (P<0.05). No PR immunostaining was observed. ER staining had been detected in endometrial glands, endometrial stoma and myometrium, however hyperplasic glands in propria mucosa had lower or no ER scores. Based on the results of this study, the high levels of prostaglandin enzymes and low ER scores in CEH could be a preliminary step for the next stages of severe differentiation of endometrium.
Identification of mast cells and immunophenotypic subtypes in peripheral nerve damage caused by epineurotomy and systemic inflammation
(Wolters Kluwer Medknow Publications, 2023-01-01) Yavaş, Özkan; YAVAŞ, ÖZKAN; Yavaş, Senem Esin; Kavruk, Kaan; KAVRUK, KAAN; Özbek, Serhat; ÖZBEK, SERHAT; Ersoy, Semiha; ERSOY, SEMİHA; Bursa Uludağ Üniversitesi/Veteriner Fakültesi; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Plastik Cerrahi Anabilim Dalı.; KHD-4075-2024
Purpose: This study aims to investigate the numerical increase, localization, granulation status, and immunophenotypic properties of mast cells (MCs) in epineurectomy-induced nerve damage and lipopolysaccharide (LPS)-induced systemic infection models. Materials and Methods: In this study, the animals were divided into three groups of 6 each. One of the groups was determined as the control group, epineurectomy was applied to one group, and systemic inflammation was created by regular LPS injections in the other group. Then, the obtained nerve tissues were stained histochemically with Hematoxylin and Eosin toluidine blue, and the increase, localization, and granulation status of MCs were examined. Immunohistochemically, antitryptase and antichymase staining were performed to determine the immunophenotypes of MCs. Results: As a result, while the number of MCs increased in both groups compared to the control group, MCs in the LPS group were in the epineurium, and MCs in the epineurotomy group were located between the nerve fibers. While MCs in the LPS group showed very severe degranulation, mild degranulation was observed in the epineurotomy group, and almost no degranulated MCs were observed in the control group. Conclusion: This study is critical because it is one of the first studies to compare MCs in different nerve damage types and examine the expression of chymase and tryptase.
Sıçanlarda deneysel olarak oluşturulan meme karsinomu ile kedi ve köpek meme tümörü olgularında mast hücrelerinin ve rollerinin incelenmesi
(Bursa Uludağ Üniversitesi, 2023-02-16) Yavaş, Özkan; Sönmez, Gürsel; Bursa Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü/Veteriner Fakültesi/Patoloji Anabilim Dalı.; 0000-0001-9811-9920
Dünya genelinde ve ülkemizde insanlarda ve hayvanlarda en yaygın ve en önemli kanser türlerinden biri olan meme kanserleri maddi ve manevi olarak ciddi kayıplara yol açmaktadır. Hastalığın erken teşhis edilememesine ve tedavi süreçlerinin zorlu, pahalı ve kesin sonuç vermemesine bağlı olarak prognostik faktörler ve erken teşhis oldukça önemli bir hal almaktadır. Bağışıklık sisteminin en önemli hücrelerinden biri olarak gösterilen mast hücreleri kanser dokularında önemli derecede arttıkları bilinse de olası rolleri ve fenotipleri henüz kesin olarak bilinmemektedir. Tez çalışmasında deneysel yolla sıçanlarda oluşturulan meme karsinomu ve kedi, köpek doğal olarak meydana gelen meme tümörü olgularında mast hücrelerinin bu bölgedeki olası yerleşim yerleri, varlıklarının tespiti, olası rolleri ve immunofenotipik özellikleri incelenmiştir. Çalışmada ayrıca TNF-alfa, MMP-9 ve PCNA ekspresyonları incelenerek mast hücreleri ile olası ilişkileri sorgulanmıştır. Deneysel olarak oluşturulan sıçan ve kedi köpek meme tümörü olgularında mast hücrelerinin sayısı normal meme dokusuna göre artış gösterdiği ve özellikle intratümöral alanda çok fazla arttıkları, PCNA ekspresyonu ile negatif korelasyon göstererek mast hücrelerinin bölgede artmasının muhtemelen canlı için iyi yönde bir etki gösterdiğini düşündürmüştür. Bu bulgular ışığında mast hücrelerinin tümör dokusunda önemli roller üstlenebileceği, prognostik açıdan yararlı olabileceği sonucuna varılmıştır.
Bir simental inekte rektal fibromatozis
(Uludağ Üniversitesi, 2018-03-26) İnan, Sevda; Özyiğit, Özgür M.; Nak, Yavuz; Yavaş, Özkan; Uludağ Üniversitesi/Veteriner Fakültesi.
Bu olayda 4 yaşlı Simental ırkı inekte rektum bölgesinde gözlenen çok sayıdaki tümöral kitlelerden dolayı rektal muayeneyi zorlaştıran ve operasyonuna karar verilen fibromatozis olgusunun klinik ve patolojik yönden incelenmesi amaçlandı. Hayvanda doğumu izleyen dönemde rektal muayene yapılmak istendiğinde rektum çevresinde zamanla sayı ve hacimleri artan kitleler gözlendi. En büyüğü 6,5 cm çapında yuvarlak ve 4x3x1,4cm büyüklüğünde oval, çeşitli büyüklükte 14 adet katı esnek kıvamda, beyaz renkli kitlelere rastlandı. Kitlelerin bazıları deri ve çoğunlukla mukoza ile kaplı olduğu dikkati çekti. %10’luk tamponlu formaldehit ile tespit edilerek parafine gömülen ve 5 µm kalınlığında kesitler alınan dokular, Hematoksilen-Eosin (H-E) ve Van Gieson ile ayrıca immunohistokimyasal (İHK) olarak sitokeratin ve vimentin ile boyandı. H-E kesitli dokulardaki tümöral kitlenin tipik anafor tarzında yerleşmiş fibroblast ve fibrositlerden oluştuğu, yapılan İHK boyamada ise vimentin pozitif ve sitokeratin negatif olarak gözlendi. Bu olgudaki gözlenen makroskobik, mikroskobik ve İHK’sal bulgular kaudal rektumda fibromatozis olgusu ile uyumlu bulunmuştur