Browsing by Author "Yibar, Artun"
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Item Detection of rope-producing bacillus in bread and ıdentification of ısolates to species level by vitek 2 system(Uludağ Üniversitesi, 2012-09-24) Yibar, Artun; Cetinkaya, Figen; Soyutemiz, Gul Ece; Uludağ Üniversitesi/Veteriner Fakültesi.This study was conducted to detect the presence of rope-producing Bacillus spp. and the rope spore counts in one hundred samples of different types of bread obtained from several bakeries and supermarket stores in Bursa province and to identify the isolates at the species level. Rope spore counts determined using the Most Probable Number (MPN) technique varied between 30 and 90 MPN/g in eight samples while this count was 11 000 MPN/g in one sample. By VITEK 2 bacterial identification system, eight of Bacillus strains isolated were identified as B. cereus and one as B. subtilis. Additionally, it was observed the growth of yeast and mould in the counts varying between 1x102 and 6.3x104 cfu/g in the samples. The results indicated that breads analysed harboured Bacillus species which can be responsible for rope spoilage of bread or food poisoning in humansPublication Effects of a short-term supplementation with liquid oligofructose-enriched inulin on faecal characteristics and selected serum metabolites of healthy saanen kids(Kafkas Üniversitesi, 2015-09-01) Kara, Cağdaş; Meral, Yavuz; Yibar, Artun; Biricik, Hakan; Orman, Abdulkadir; Deniz, Gülay; Yeşilbağ, Derya; Çetin, İsmail; Gençoğlu, Hıdır; Türkmen, İsmet; KARA, ÇAĞDAŞ; Meral, Yavuz; YIBAR, ARTUN; BİRİCİK, HAKAN; ORMAN, ABDÜLKADİR; DENİZ, GÜLAY; YEŞİLBAĞ, DERYA; ÇETİN, İSMAİL; GENÇOĞLU, HIDIR; TÜRKMEN, İBRAHİM İSMET; Uludağ Üniversitesi/Veteriner Fakültesi/Hayvan Besleme ve Beslenme Hastalıkları Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Gıda Hijyeni ve Teknolojisi Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Zootekni Anabilim Dalı.; 0000-0001-7051-1349; 0000-0001-9138-4422; AAH-4032-2021; ABE-9200-2022; AAG-9134-2021; AAK-5370-2020; HJZ-4775-2023; FFA-7187-2022; DGQ-7149-2022; ISX-0239-2023; JIM-4044-2023; EDZ-7334-2022The objective of this study was to evaluate the effects of a short-term supplementation with liquid oligofructose-enriched inulin on faecal characteristics and selected serum metabolites of healthy Saanen kids. Twenty-four kids (44 days of age) were allotted to a control (CG) or an experimental (EG) group. Each group consisted of 12 kids. Each kid in EG was supplemented with 0.8 and 1.6 g/d of oligofructose-enriched inulin from day 1 to 5 and from day 6 to 15, respectively. Liquid oligofructose-enriched inulin supplementation did not affect faecal score and pH (P > 0.05). Faecal acetate, propionate and total SCFA concentrations did not differ (P > 0.05) between CG and EG, whereas faecal butyrate concentration was higher (P< 0.05) in kids supplemented with liquid oligofructose-enriched inulin. Due to trophic and antiinflammatory effects of butyrate, we hypothesize that oligofructose-enriched inulin supplementation may be useful to help tissue repair and regeneration, particularly during an intestinal infection. Faecal Lactobacillus, Bifidobacterium and Clostridium perfringens concentrations were not affected by oligofructose-enriched inulin supplementation (P > 0.05). Daily dose of oligofructose-enriched inulin tended to increase serum glucose concentrations (P < 0.09, P < 0.08). Serum urea and albumin concentrations were similar between groups (P > 0.05). Serum total protein and globulin levels were lower in EG compared with CG (P < 0.05). During the experimental period lasting for 15 days, there were no differences in growth performance parameters between groups (P > 0.05).Item Effects of boiling on nitrofuran AOZ residues in commercial eggs(Kafkas Üniversitesi, 2013-11) Okutan, Bülent; Yibar, Artun; Güzel, Saime; Uludağ Üniversitesi/Veterinerlik Fakültesi/Gıda Hijyeni ve Teknolojisi Bölümü.; Uludağ Üniversitesi/Veterinerlik Fakültesi/Biyokimya Anabilim Dalı.; 0000-0003-0796-5000; AAH-4275-2021; 38762296900; 55460886200The aim of this study was to determine the effects of boiling on nitrofuran 3-amino-2-oxazolidinone (AOZ) residues in eggs. The use of furazolidone in food-producing animals is banned within the EU and Turkey. The nitrofuran AOZ residues in raw and boiled eggs were analysed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in a chromotographic run of 20 min. The method validation was done according to the criteria laid down in Commission Decision No. 2002/657 EC. Linearity was proved between 0 to 1.5 mu g/kg, decision limit (CC alpha) was 0.70 mu g/kg, detection capability (CC beta) was 0.77 mu g/kg, recovery values ranged between 88-97.9% and repeatability (CV) was 3-4.3%. The detected avarage nitrofuran AOZ residue level in 13 uncooked eggs by LC-MS/MS was 0.86 +/- 0.017 mu g/kg which was increased to 2.42 +/- 0.037 mu g/kg after boiling. In this study, it was surprisingly found that protein-bound side-chain metabolite, nitrofuran AOZ levels in eggs were significantly increased after boiling. This finding runs counter to the claim that heat process in general should decrease various antibiotic levels in food. The observed increase (P<0.001) in nitrofuran AOZ levels in boiled eggs relative to uncooked eggs may be due to enhanced efficiency of extraction in boiled samples. Therefore boiled eggs should be used for analysis of nitrofuran AOZ levels in order to obtain more reliable and more predictive results.Item Effects of inulin supplementation on selected faecal characteristics and health of neonatal Saanen kids sucking milk from their dams(Elsevier, 2012-12) Kara, Çağdaş; Orman, Abdülkadir; Gençoğlu, Hıdır; Kovanlıkaya, Arda; Meral, Yavuz; Çetin, İrene; Yibar, Artun; Kasap, Sevim C.; Türkmen, İbrahim İsmet; Deniz, Gülay; Uludağ Üniversitesi/Veteriner Fakültesi/Hayvan Besleme ve Beslenme Hastalıkları Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Zooteknik Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Gıda Hijyeni ve Teknolojisi Bölümü.; Uludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı.; 0000-0002-9217-3034; 0000-0001-9138-4422; AAC-2909-2020; AAP-7998-2020; AAG-9134-2021; K-6112-2016; 31967678500; 24335834100; 15753357900; 31967682500; 55786609400; 7006082971; 38762296900; 36514844800; 6701855392; 8849978100Fifty newborn Saanen kids were used to study the effects of inulin supplementation on faecal score, faecal pH, selected faecal bacterial population, BW, body temperature, haematological traits, selected health parameters and the incidence of diarrhoea. Kids were sorted by parity of their dams and multiple birth (twin or triplet) and assigned to one of the two groups (control: CG, and experimental: EG) at birth. Each group consisted of 25 kids. The groups were similar with regard to sex and birth weight. All kids were fed colostrum for the first 3 days after birth, and then the kids in EG were adapted to inulin supplementation by an increased dosage from day 4 to 7. Each kid in EG was supplemented with 0.2 g, 0.3 g, 0.4 g, 0.5 g and 0.6 g inulin on day 4, 5, 6, 7 and from day 8 to 28, respectively, whereas the kids in CG did not receive inulin. Faecal score and faecal bacterial population were not affected by inulin supplementation (P > 0.05). There were differences in faecal pH on day 14 (P = 0.01) and 28 (P < 0.05), whereas no difference in faecal pH on day 21 (P > 0.05) was detected between groups. No differences (P > 0.05) in BW and haematological traits were found between groups. Body temperature did not differ on day 14 and 21 (P > 0.05), whereas there was a difference in body temperature on day 28 (P = 0.01) between groups. The numbers of kids with pneumonia and kids treated for pneumonia and diarrhoea were similar for CG and EG. Kid losses during the study were the same for CG and EG. The incidence of diarrhoea was not affected by inulin supplementation (P > 0.05). Inulin supplemented to kids did not adversely affect faecal score. The effect of inulin on faecal pH was not consistent. The results of our study suggested that daily dose (0.6 g) of inulin might not be enough to observe effects of it. Our data will be useful to determine the dose and timing of inulin supplementation in future studies investigating the effects of inulin on the parameters associated with performance and health status in kids and other young ruminants.Publication Extended spectrum beta-lactamase activity and multidrug resistance of Salmonella serovars isolated from chicken carcasses from different regions of Turkey(Ankara Üniversitesi, 2015-01-01) Ata, Zafer; Dinç, Gökcen; Yibar, Artun; Mustak, Hamit Kaan; Şahan, Özlem; Ata, Zafer; YIBAR, ARTUN; Uludağ Üniversitesi/Veteriner Fakültesi/Gıda Hijyeni ve Teknolojileri Bölümü.; 0000-0001-9510-5734; W-7994-2019; ABE-9200-2022This research was conducted to investigate the extended spectrum beta-lactamase activity and multidrug resistance of Salmonella serovars isolated from chicken carcasses. For this purpose, 99 Salmonella isolates from 930 chicken carcasses were tested against 12 different antimicrobials. The resistance rates of Salmonella isolates to antimicrobials were as follows: 35.3% (35/99) to ampicillin, 33.3% (33/99) to tetracycline, 29.2% to amoxicillin-clavulanic acid, 18.1% (18/99) to nalidixic acid, 17.1% (17/99) to chloramphenicol, 16.1% (16/99) to aztreonam, 12.1% (12/99) to trimethoprim-sulfamethoxazole, 4% (4/99) to gentamicin, 1.0% (1/99) to ceftazidime. Of the isolates 46.4% (46/99) were found to be resistant to two or more antimicrobials as a multidrug resistance. Extended spectrum beta-lactamase activity was detected in 1.0% (1/99) of the isolates. Furthermore, S. Typhimurium 26.2% (28/99), S. Infantis 16.1% (16/99), S. Hadar 12.1% (10/99) and S. Branderburg 9.0% (9/99) were found to be the predominant serovars. In conclusion, antimicrobial resistance and also multidrug resistance rates of Salmonella isolates in this study, indicated that monitoring of antimicrobial resistance profiles is important for Salmonella infections to plan treatment strategies.Item The effect of volatile oil mixtures on the performance and ilio-caecal microflora of broiler chickens(Taylor & Francis, 2016-04-28) Çetin, Ece; Yibar, Artun; Yeşi̇lbağ, Derya; Çetin, İrene; Cengiz, Şerife Şule; Uludağ Üniversitesi/Veteriner Fakültesi/Gıda Hijyeni ve Teknolojisi Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Hayvan Besleme Anabilim Dalı.; 0000-0003-0708-3833; 0000-0002-9217-3034; AAK-5370-2020; B-1526-2018; 57200978804; 38762296900; 9246817500; 56329475100; 70060829711. The aim of this study was to investigate the individual and combined effects of rosemary, oregano and fennel volatile oil (FVO) supplementation on the performance and ilio-caecal bacteriological flora of broiler chickens.A total of 800 male Ross-308 broiler chickens were divided equally into 8 groups; each contained 100 chickens. The study included a control treatment (NC) with no dietary additives that was supplemented with oils according to the following 7 treatments: 200mg -tocopherol acetate/kg (PC), 100mg oregano volatile oil (OVO)/kg, 100mg rosemary volatile oil (RVO)/kg, 100mg FVO/kg and an equal mixture of oregano+rosemary+fennel VO (100, 200, 400mg/kg, VOM-1, VOM-2 and VOM-3, respectively). The experiment lasted for 6weeks.At the end of the experiment, dietary supplementation with -tocopherol, oregano, rosemary and FVO and two different volatile oil mixtures (VOMs) (VOM-2; VOM-3) significantly increased the body weights (BWs) of broilers at 7, 14 and 21d of age compared to the negative control (NC) (-) and VOM-1 groups. At 0-42d, birds fed on VOM-3 were considerably heavier and also gained more weight than NC (-) and VOM-1 groups.The blend of VOs at 400mg/kg significantly increased Lactobacillus spp. in faeces. The blends of oregano, rosemary and FVOs (VOM-3) at 400mg/kg concentration and also VOM-3 group exhibited stronger antibacterial activity against coliform bacteria compared to the NC (-) group.In conclusion, the blend of oregano, rosemary and fennel VOs at higher concentrations (400mg/kg concentration) in diets can be used to stimulate the growth and can improve the intestinal microbial balance (including a reduction of coliform bacteria and an increase in Lactobacillus spp. counts) of broiler chickens.