Browsing by Author "Ziyanok, Sedef"

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Effects of green tea on serum paraoxonase/arylesterase activities in streptozotocin-induced diabetic rats
(Pergamon-Elsevier Science, 2005-12) Taş, Sibel; Sarandöl, Emre; Ziyanok, Sedef; Aslan, Kemal; Dirican, Melahat; Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Biyoloji Bölümü.; Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; 0000-0002-2593-7196; AAH-4272-2021; ABE-1716-2020; AAG-6985-2021; ABE-6873-2020
In recent years, green tea has become a subject of interest because of its beneficial effects on human health. The purpose of this study was to determine the effects of green tea on serum paraoxonase/arylesterase activities and lipoprotein oxidizability in streptozotocin-induced diabetic rats (65 mg/kg [intraperitoneal]). Green tea was given in tap water (2%) for 3 and 6 weeks to control (CGT-3w and CGT-6w) and diabetic (DGT-3w and DGT-6w) rats, and they were compared with the control and diabetic groups (D-3w and D-6w), respectively. Serum insulin level was significantly increased in the DGT-6w group; serum lipid and plasma and tissue malondialdehyde levels were reduced in the DGT-3w and DGT-6w groups. Oxidizability of apolipoprotein B-containing lipoprotein fraction was found to be significantly reduced in the DGT-6w group. Serum total antioxidant capacity showed a significant increase in the CGT-6w and DGT-6w groups. Paraoxonase activity was significantly reduced in the D-3w and D-6w groups and increased in the DGT-6w group. We conclude that green tea might have antilryperlipidernic and antioxidative effects and may slow the progression of atherogenesis by reducing oxidation of lipoproteins and preserving paraoxonase activity.
In vivo systemic chlorogenic acid therapy under diabetic conditions: Wound healing effects and cytotoxicity/genotoxicity profile
(Pergamon-Elsevier Science, 2015-07) Bağdaş, Deniz; Etöz, Betül Cam; Gül, Zülfiye; Ziyanok, Sedef; İnan, Sevda; Turaçözen, Özge; Gül, Nihal; Topal, Ayşe B.; Çinkılıç, Nilüfer; Taş, Sibel; Özyiğit, Musa Özgür; Gürün, Mine Sibel; Uludağ Üniversitesi/Tıp Fakültesi/Deney Hayvanları Yetiştirme ve Araştırma Merkezi.; Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji Anabilim Dalı.; Uludağ Üniversitesi/Fen ve Edebiyat Fakültesi/Biyoloji Bölümü.; Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Cerrahi Anabilim Dalı.; 0000-0002-8872-0074; 0000-0002-3595-6286; 0000-0001-8138-5851; AAR-6478-2021; AAF-9939-2020; AAG-8716-2019; E-3364-2018; ABE-6873-2020; AAH-4272-2021; AAH-2873-2021; AAH-5296-2021; 15062425700; 56427863700; 56086542900; 9735158200; 56320836200; 56320248500; 8387784600; 56357211200; 26533892300; 7004343411; 6507338060; 55664349700
Oxidative stress occurs following the impairment of pro-oxidant/antioxidant balance in chronic wounds and leads to harmful delays in healing progress. A fine balance between oxidative stress and endogenous antioxidant defense system may be beneficial for wound healing under redox control. This study tested the hypothesis that oxidative stress in wound area can be controlled with systemic antioxidant therapy and therefore wound healing can be accelerated. We used chlorogenic acid (CGA), a dietary antioxidant, in experimental diabetic wounds that are characterized by delayed healing. Additionally, we aimed to understand possible side effects of CGA on pivotal organs and bone marrow during therapy. Wounds were created on backs of streptozotocin-induced diabetic rats. CGA (50 mg/kg/day) was injected intraperitoneally. Animals were sacrificed on different days. Biochemical and histopathological examinations were performed. Side effects of chronic antioxidant treatment were tested. CGA accelerated wound healing, enhanced hydroxyproline content, decreased malondialdehyde/nitric oxide levels, elevated reduced-glutathione, and did not affect superoxide dismutase/catalase levels in wound bed. While CGA induced side effects such as cyto/genotoxicity, 15 days of treatment attenuated blood glucose levels. CGA decreased lipid peroxidation levels of main organs. This study provides a better understanding for antioxidant intake on diabetic wound repair and possible pro-oxidative effects.
Pharmacologic overview of systemic chlorogenic acid therapy on experimental wound healing
(Springer, 2014-11-01) Bağdaş, Deniz; Gül, Nihal Yaşar; Topal, Ayşe; Taş, Sibel; Özyiğit, Musa Özgür; CinkIlıç, NilÜfer; Gül, Zülfiye; Etoz, Betül Cam; Ziyanok, Sedef; İnan, Sevda; Turacozen, Özge; Gürün, Mine Sibel; Bağdaş, Deniz; GÜL SATAR, NİHAL YAŞAR; TOPAL, AYŞE; TAŞ, SİBEL; ÖZYİĞİT, MUSA ÖZGÜR; CinkIlıç, Nilüfer; Gül, Zülfiye; Etoz, Betül Cam; ZİYANOK DEMİRTAŞ, SEDEF; İnan, Sevda; Turacözen, Özge; GÜRÜN, MİNE SİBEL; Uludağ Üniversitesi/Tıp Fakültesi/Deney Hayvanları Yetiştirme ve Araştırma Merkezi; Uludağ Üniversitesi Veteriner Fakültesi, Cerrahi Anabilim Dalı; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü; Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji Anabilim Dalı; 0000-0001-6225-774X; 0000-0002-3595-6286; 0000-0002-8872-0074; 0000-0003-3878-3808; 0000-0001-8138-5851; AAG-8716-2019; AAH-4272-2021; AAF-9939-2020; ABE-6873-2020; JBJ-7162-2023; AAH-5296-2021; AAR-6478-2021; AAH-2873-2021; E-3364-2018; EOB-5882-2022; GGO-6894-2022; AAH-5296-2021
Chlorogenic acid (CGA) is a well-known natural antioxidant in human diet. To understand the effects of CGA on wound healing by enhancing antioxidant defense in the body, the present study sought to investigate the potential role of systemic CGA therapy on wound healing and oxidative stress markers of the skin. We also aimed to understand whether chronic CGA treatment has side effects on pivotal organs or rat bone marrow during therapy. Full-thickness experimental wounds were created on the backs of rats. CGA (25, 50, 100, 200 mg/kg) or vehicle was administered intraperitoneally for 15 days. All rats were sacrificed on the 16th day. Biochemical, histopathological, and immunohistochemical examinations were performed. Possible side effects were also investigated. The results suggested that CGA accelerated wound healing in a dose-dependent manner. CGA enhanced hydroxyproline content, decreased malondialdehyde and nitric oxide levels. and elevated reduced glutathione, superoxide dismutase, and catalase levels in wound tissues. Epithelialization, angiogenesis, fibroblast proliferation, and collagen formation increased by CGA while polymorph nuclear leukocytes infiltration decreased. CGA modulated matrix metalloproteinase-9 and tissue inhibitor-2 expression in biopsies. Otherwise, high dose of CGA increased lipid peroxidation of liver and kidney without affecting the heart and muscle samples. Chronic CGA increased micronuclei formation and induced cytotoxicity in the bone marrow. In conclusion, systemic CGA has beneficial effects in improving wound repair. Antioxidant, free radical scavenger, angiogenesis, and anti-inflammatory effects of CGA may ameliorate wound healing. High dose of CGA may induce side effects. In light of these observations, CGA supplementation or dietary CGA may have benefit on wound healing.
Tip 2 diyabet oluşturulmuş sıçanlarda silimarin, oleuropein ve saksagliptinin oksidan–antioksidan sistemler üzerine etkisi
(Uludağ Üniversitesi, 2014-05) Ziyanok, Sedef; Taş, Sibel; Uludağ Üniversitesi/Fen Bilimleri Enstitüsü/Biyoloji Anabilim Dalı.
Diyabetes mellitus'ta kan glukoz ve lipit düzeylerindeki artış sonucu oluşan oksidatif stres, diyabete bağlı komplikasyonların gelişmesinde önemli rol oynamaktadır. Olea europaea (zeytin) yaprağı ve Silybum marianum (devedikeni = Meryem ana) ekstratı ve Saksagliptin kan glukozu ve lipit seviyelerini düşürücü ve ayrıca antioksidan özelliklerine bağlı olarak oksidatif stresi azaltabilir. Bu çalışmada, streptozotosin-nikotinamit ile tip 2 diyabet oluşturulmuş sıçanlarda oleuropein, silimarin ve saksagliptinin hipoglisemik, hipolipidemik, oksidan ve antioksidan sistemler üzerine etkisi araştırıldı. Nikotinamitin (45mg/kg) intraperitoneal enjeksiyonundan 15 dk sonra streptozotosin (65mg/kg) enjeksiyonu ile tip 2 diyabet oluşturuldu. Olea europaea ve Silybum marianum ekstraktları (%1) ve saksagliptin (1.5mg/gün) içme suyuna 5 hafta süre ile eklendi. 100 adet Wistar türü erkek sıçanlar rastgele kendi aralarında on gruba ayrıldı; kontrol (K), kontrol + Olea europaea ekstraktı (K + OEE), kontrol + Silybum marianum ekstraktı (K + SME), kontrol + Olea europaea + Silybum marianum ekstraktı (K + OEE + SME), diyabet (D), diyabet + Saksagliptin (D + S), diyabet + Olea europaea ekstraktı (D + OEE), diyabet + Silybum marianum ekstraktı (D + SME), diyabet + Olea eruopea + Silybum marianum ekstraktı (D + OEE + SME), diyabet + Saksagliptin + Olea europaea + Silybum marianum ekstraktı (D + S + OEE + SME).K + OEE grubunda K grubuna göre kan süperoksit dismutaz, glutatyon peroksidaz ve aril esteraz enzim aktivitesinde anlamlı artış, K +SME grubunda K grubuna göre yüksek dansiteli lipoprotein, kan süperoksit dismutaz, glutatyon peroksidaz ve aril esteraz enzim aktivitesinde anlamlı artış, K + OEE + SME grubunda K grubuna göre total kolesterol seviyesinde anlamlı azalma, yüksek dansiteli lipoprotein, kan süperoksit dismutaz, glutatyon peroksidaz, paraoksonaz ve aril esteraz enzim aktivitesinde anlamlı artış saptandı. D + S grubunda D grubuna göre serum total kolesterol, trigliserit, kan glukoz düzeylerinde anlamlı azalma, plazma gastrik inhibitör peptit, glukagon benzeri peptit-1, yüksek dansiteli lipoprotein, serum insülin, kan süperoksit dismutaz, glutatyon peroksidaz ve aril esteraz enzim aktivitesinde anlamlı artış, D + OEE, D + SME, D + OEE + SME ve D + S + OEE + SME gruplarının her birinde D grubuna göre serum total kolesterol, trigliserit, kan glukoz, doku (kalp ve karaciğer) malondialdehit seviyelerinde anlamlı azalma, yüksek dansiteli lipoprotein, serum insülin, kan süperoksit dismutaz, glutatyon peroksidaz ve aril esteraz enzim aktivitesinde anlamlı artış saptandı. Sonuç olarak çalışmamızda, bir dipeptidil peptidaz inhibibitörü olan saksagliptin tedavisiyle birlikte, Olea europaea ve Silybum marianum ekstraktlarının antihiperglisemik, antihiperlipidemik ve antioksidan özelliklerinden dolayı tip 2 diyabette oluşan oksidatif strese karşı koruyucu ve/veya önleyici etkisinin olduğu ve diyabette tedaviye destek amaçlı kullanılmasının yararlı olabileceği sonucuna varıldı.