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Browsing by BUU Author "Abdou, Maoulida"

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    Lipase from new isolate bacillus cereus ATA179: Optimization of production conditions, partial purification, characterization and its potential in the detergent industry
    (Tübitak Bilimsel ve Teknolojik Araştırma Kurumu, 2021-01-01) Demirkan, Elif; Aybey Çetinkaya, Aynur; Abdou, Maoulida; DEMİRKAN, ELİF; Aybey Çetinkaya, Aynur; Abdou, Maoulida; Fen Edebiyat Fakültesi; Biyoloji Bölümü; 0000-0002-5292-9482; 0000-0003-2743-9745; ABI-4472-2020; CJN-1872-2022; CAU-1487-2022
    In this study, 341 Bacillus sp. strains were isolated from agricultural soils of Turkey. The potent extracellular lipase producer was selected. It was identified by 16S rRNA, named as Bacillus cereus ATA179. Optimal nutritional and physical parameters for lipase production were determined. Sucrose as the carbon source, (NHIHPO, as the nitrogen source, CaCl2 as the metal ion were obtained. The best results of physical parameters were stated at 45 degrees C, pH 7.0, shaking rate 50 rpm, inoculation amount 7% and inoculum age 24 h. ATA179 strain showed a 51% increase in enzyme production in the modified medium created by optimizing nutritional and physical conditions. Optimum pH value and temperature were found as 6.0 and 55 degrees C, respectively. CaCl2, Tween 20, Triton X-100 had an activating effect on enzyme activity. V-max and K-m kinetic values were found as 18.28 U/mL and 0.11 mM, respectively. The molecular weight was determined as 47 kDa. Lipase was found to be stable up to 75 days at -20 degrees C. The potential of the enzyme in detergent industry was also investigated. It was not affected by detergent additives, but was found to be effective in removing oils from contaminated fabrics. This new lipase may have potential to be used in detergent industry.
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    Optimization of culture medium for the production and partial purification and characterization of an antibacterial activity from brevibacillus laterosporus strain EA62
    (Ars Docendi, 2019-07-01) Usta, Alev A. K.; Demirkan, Elif; Cengiz, Murat; Sevgi, Tuba; Zeren, Behice; Abdou, Maoulida; Usta, Alev A. K.; DEMİRKAN, ELİF; CENGİZ, MURAT; SEVGİ, TUBA; Zeren, Behice; Abdou, Maoulida; Veteriner Fakültesi; Biyoloji Bölümü; 0000-0002-5292-9482; ABI-4472-2020; ABE-5935-2020; IPA-7484-2023; ISY-3462-2023; EID-6407-2022; CAU-1487-2022
    The present study was performed to report the bacterial identification, optimization of culture conditions and characterization of a novel antibiotic substance from a Bacillus sp. EA62 strain isolated from soil. The EA62 strain was identified based on 16S rRNA analysis. The new isolate EBD 9-1 showed 100% sequence identity with Brevibacillus laterosporus. The antibacterial activity of the new substance was examined against five pathogenic bacteria and was observed to be the most effective against Escherichia coli. An agar diffusion assay was performed to evaluate the antibacterial activity of the substance. The effects of some nutritional (amino acid, carbon, nitrogen and metal sources) and physical factors (pH and temperature) and incubation time on the antibiotic activity were studied. Antibiotic activity in basal medium reached the maximum levels after 72 h of incubation. The best antibiotic activity was obtained in the presence of glucose as a carbon source, yeast extract as a nitrogen source, glutamic acid as an amino acid source and MgSO4+CaCO3 as metal ion sources. For physical parameters, the best results were obtained at 37 degrees C, pH 7.0. The antibiotic substance was partially purified, and the estimated molecular weight was 6.3 kDa. The minimum inhibitory concentration (MIC) values determined against five pathogen bacteria were >256 mu g/ml. The substance was identified by thin-layer chromatography, and its Rf value was measured as 0.04 cm.