2023 Cilt 42 Sayı 1

Permanent URI for this collectionhttps://hdl.handle.net/11452/41030

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Characterization and computational ınvestigation of polymorphisms in the TIR domain of the TLR4 protein in holsteins raised in Turkey
(Bursa Uludağ Üniversitesi, 2023-03-01) Atalay, Sertaç
Increasing environmental temperatures due to climate change adversely affect livestock welfare and health. Moreover, temperatures increase thedistribution and survival of parasites and infectious agents. Livestock diseases that cause significant economic losses are a worldwide concern.Toll-like receptor 4 (TLR4) is an ideal marker gene candidate, due to its critical role it plays in initiating the immune response against pathogens.In this study, the toll-interleukin-1 receptor (TIR) domain polymorphisms were investigated in the Holsteins raised in Turkey. by DNAsequencing. The effects of polymorphisms on the protein structure and function were evaluated by computational tools (I-Mutant Suite, ProjectHope and PyMOL). The rs8193069 C>T polymorphism was detected in the TIR domain. The SNP causes Threonine to Isoleucine substitutionat position 674 of the TLR4 protein which is the second amino acid of the TIR domain. The I-Mutant Suite predicted that Thr674Ile substitutioncould decrease protein stability (DDG= -0.40 Kcal/mol). Project Hope results showed that mutant and wild-type amino acids have differentproperties and that this can disturb the TIR domain. The Thr674Ile polymorphism observed in the TIR domain of TLR4 protein in Holsteinsraised in Turkey might affect the function of the TLR4 by causing physicochemical changes.
Comparison of beta-hydroxybutyrate levels in the first three weeks after calving in four healthy different breeds of cows
(Bursa Uludağ Üniversitesi, 2023-01-18) CİHAN, HÜSEYİN; KASAP, SEVİM; ŞENTÜRK, SEZGİN; Veteriner Fakültesi; İç Hastalıkları Ana Bilim Dalı; 0000-0003-1544-6438; 0000-0001-9501-9983; 0000-0002-2465-9913
The objective of this study was to determine the differences between the healthy Holsteın-Friesian, Jersey, Simmental, and Aberdeen Angusbreeds Beta-hydroxybutyrate (BHBA) levels at the first three weeks after calving. The blood samples were collected for analysis between 10-20 days postpartum. In this study, 80 animals were used in different regions in total. BHBA was analyzed by Ketosite® (Ketosite®, Stanbio LabrotoryInc., Boerne, TX USA). Milk fat and protein levels were immediately measured by an electronic milk analyzer (Milkoscan FT1 FOSS Industries, Denmark). Statistical analyses of the study were performed using Sigma Plot 12 (Systat Software Inc., San Lose, CA). The means of BHBA levelswere compared by One Way Anova. As in Holstein cows, blood BHBA levels increased in the early lactation period and also in other breeds. Theresults of this study presented that serum BHBA levels were significantly higher in Holsteın-Friestein cows than in Simmental and AberdeenAngus cows.
Comparison of glucose levels in blood samples taken from coccygeal, jugular and mammary vein of cows in lactation
(Bursa Uludağ Üniversitesi, 2023-01-23) Akgül, Gülşah; Cihan, Hüseyin; Kasap, Sevim; Evci, Fatma Zehra; Şentürk, Sezgin; Veteriner Fakültesi; İç Hastalıkları Bölümü; 0000-0003-1544-6438; 0000-0001-9501-9983; 0000-0002-2767-7690; 0000-0002-2465-9913
Blood glucose level is one of the most commonly used parameters in assessing the energy metabolism in dairy cows. The presented study aimed to compare the glucose concentrations in blood samples taken from the coccygeal vein, jugular vein, and mammary vein from the sameanimals at the same time. Healthy Holstein-Friesian dairy cows (n=25) in their second and third lactation were selected for the study. Theblood samples were taken from the coccygeal vein, jugular vein, and V. subcutanea abdominis (mammary vein) at the same time from eachanimal. To analyze blood glucose, Free Style Optium Blood Glucose Test Strip (Abbott, Istanbul, TURKIYE) was used. In comparison to theblood taken from both the jugular vein and coccygeal vein, glucose levels in blood samples taken from the mammary vein were significantly low (P<0.001). As a result, the determination of glucose levels in blood samples taken from the mammary veins may cause erroneous interpretations.The refore, the mammary vein for blood glucose must not be used as a sampling site.
Gender determination by PCR assay for the sex-determining region Y(SRY) gene amplification in linnaeus’s two-toed sloth (Choloepus Didactylus)
(Bursa Uludağ Üniversitesi, 2023-06-15) Bozkurt, Berkay; Vatansever, Ezgi; ARDIÇLI, SENA; Veteriner Fakültesi; Genetik Ana Bilim Dalı; 0000-0003-2758-5945
In Linnaeus’s two-toed sloths (Choloepus didactylus), there is no distinct sexual dimorphism. It is an obstacle for gender determination fromthe external genitalia, especially in newborns or young sloths. Hence, easy, rapid, and reliable genetics-based methods for gender identificationof the sloths are needed to continue captive breeding more successfully. In this study, a PCR-based technique that allows gender determinationof two-toed sloths by using a sex-determining region Y (SRY) gene marker was described. The hair samples from young (suspectgender) and adult sloths (known gender) were used in genetic analysis. Initially, genomic DNA was isolated from hair root samples using Roche high pure PCR template preparation kit. The SRY primers were specifically designed based on the NCBI and Ensembl databases, andthey were verified with the BLAST program concerning the two-toed sloth genome. PCR amplification with the SRY-specific primers wascarried out by a programmable thermal cycler device using FastStart High Fidelity PCR System, Roche dNTPack. The samples were then electrophoresedon 2% agarose gels and were visualized by a gel documentation and analysis system. A specific band in the electrophoresis patternis diagnostic for a male individual with a partial SRY region. Hence, the analysis demonstrated that the samples belonged to a male two-toedsloth. Two-toed sloth species are commonly preferred animals in zoos. Gender determination is inevitable for these animals in captivity tobe raised successfully and healthily. Molecular genetic techniques allow high efficiency in taxonomic evaluations and gender identification inspecies that do not display sexual dimorphism. The PCR assay described here may be helpful for a rapid genetic analysis that can be widelyused in gender determination for two-toed sloths.
Preventive effects of bovine gallstone on murine colon neoplasia: Room for improvement?
(Bursa Uludağ Üniversitesi, 2023-05-04) Demirkan, İbrahim; Kavas, Ece; Bozkurt, Mehmet; Korkmaz, Musa; Kılıç, İbrahim; Kavas, Taner
Bovine gallstone (BGS) has been suggested to be used in the treatment of many diseases including neoplastic maladies in traditional medicine.Thus, this study aimed at evaluating the possible protective effects of BGS in mice colon tumor models. In the experiment, 8-week-old 30 male BALB/c mice weighing 25-30 g were randomly allocated into the control group (n=6), tumor group ((n=6; tumor induced by 1,2- dimethylhydrazine?[DMH]), BGS>DMH group (n=6; BGS given before DMH challenge), BGS//DMH group (n=6; simultaneous administration of BGS andDMH) and DMH>BGS group (n=6; tumor induced by DMH then BGS given). The colonic tumor was induced by weekly subcutaneous injectionof DMH at a 20 mg/kg/mouse dose for consecutive 11 weeks. BGS preparations were given to mice cyclically. Atypical cryptic foci formation inthe colon was examined by histopathological staining. The lowest rate of colon tumor was noted in BGS>DMH group where BGS was given priorto tumor challenge. In addition, simultaneous administration of DMH and BGS to mice inhibited tumor formation and development more significantlythan those in other groups (except for BGS>DMH group). It was concluded that BGS significantly prevented colonic tumor formationif given to subjects before or during tumor challenge however, therapeutic capacity of BGS was not promising in this study.

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