Person: ÖZYİĞİT, MUSA ÖZGÜR
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ÖZYİĞİT
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MUSA ÖZGÜR
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Publication Her-2 positivity rate in dogs with mammary carcinoma: A systematic review and meta-analysis(Hellenic Veterinary Medical Soc, 2020-04-01) Uzabacı, Ender; UZABACI, ENDER; Özyiğit, Musa Özgür; ÖZYİĞİT, MUSA ÖZGÜR; Ercan, İlker; Arda, O.; Veteriner Fakültesi; Patoloji Ana Bilim Dalı; 0000-0002-9634-0055; 0000-0002-2382-290X; JPL-5273-2023; ABF-2367-2020; AAR-6478-2021Human epidermal growth factor receptor 2 (HER-2) plays an essential role in cell growth and survival. HER-2 overexpression occurs in 20-30% of human breast tumors and has prognostic value as it is associated with disease progression. HER-2 overexpression is also associated with tumor progression and metastasis in malignant mammary tumors of the canine. However, in the literature, different positivity classifications/scoring were used in the evaluation of HER-2 status, and there is no consensus in terms of scoring of HER-2 expression in canine mammary tumors. In this study, it was aimed to estimate the HER-2 positivity rate by evaluating the results of the study using different positivity classifications by meta-analysis. In this context, by using "HER-2 canine mammary tumor" keywords, Pubmed and Web of Science electronic databases were scanned until February 2019, and a total of 97 related studies were found. However, 20 of these studies were used for the analysis. Two different meta-analyses were performed to evaluate the HER-2 positivity status with "2+ and 3+" and "3+" scores. As a result, HER-2 positivity rates were determined at 25.87% and 25.99% for the studies using "2+/3+" scores and "3+" respectively for HER-2 positivity. Therefore, this result suggests that the rate of HER-2 positivity is similar between humans and dogs.Publication Evaluation of serum anti-Mullerian hormone concentrations following treatment with vitamin D in Holstein Friesian heifers(Wiley, 2023-10-03) Koca, Davut; Nak, Yavuz; Şendağ, Sait; Nak, Deniz; Avcılar, Talha; Şahin, Mustafa Eren; Arslan, Talha; Özyiğit, Musa Özgür; Wehrend, Axel; NAK, YAVUZ; NAK, DENİZ; Avcılar, Talha; Şahin, Mustafa Eren; ÖZYİĞİT, MUSA ÖZGÜR; Veteriner Fakültesi; Patoloji Ana Bilim Dalı; IZN-9713-2023; LRU-4161-2024; EMG-5035-2022; IOB-6085-2023; JJX-7351-2023Anti-Mullerian hormone (AMH) levels in the blood of dairy cattle are associated with the count of antral follicles (AFC), ovarian function. Numerous studies, particularly in human medicine, have shown that vitamin D3 (Vit D3) has a positive effect on AMH levels. To our knowledge, the effects of Vit D3 on serum AMH levels in heifers have not been investigated. The goal of the current study was to evaluate the concentrations of serum AMH in dairy heifers following Vit D3 treatment. The study included 20 healthy non-pregnant Holstein Friesian heifers. These animals came to estrus at least once. All heifers received a single intramuscular dosage of 5 million IU Vit D3 (Cholecalciferol). Blood samples were collected from the coccygeal veins of all animals before and after Vit D3 injection (days 7, 14, and 28) for the measurement of AMH and 25(OH)D concentrations. In our analysis, we observed that after exogenous Vit D3 administration, circular 25(OH)D increased constantly (up to day 28 after Vit D3 injection) in all animals. On day 28, AMH concentrations saw a 10% increase in comparison with those measured the day before the Vit D3 injection. However, a comparison of AMH concentrations measured across days did not reveal statistically significant differences between Day 0, 7, 14, and 28 levels (p = .10). Furthermore, no statistical correlation was observed between the pairs (age-AMH) and (weight-AMH). Considering all times, no correlation was found between 25(OH)D and blood AMH levels. These findings demonstrated that exogenous Vit D3 did not affect serum AMH in Holstein Friesian heifers.Publication Expression profile of prostaglandin enzymes in cystic endometrial hyperplasia in dogs: The results of a hypothesis in clinical trial(Kafkas Üniversitesi, 2023-01-30) Korlu, Yeşim; Yavaş, Özkan; Aktar, Ahmet; Bozkurt, Berkay; Özyiğit, Musa Özgür; Özalp, Gözde Rabia; Korlu, Yeşim; YAVAŞ, ÖZKAN; Aktar, Ahmet; ÖZYİĞİT, MUSA ÖZGÜR; ÖZALP, RABİA GÖZDE; Veteriner Fakültesi; Jinekoloji ve Doğum Ana Bilim Dalı; 0000-0002-2975-2594; AAE-3607-2019; KHD-4075-2024; CZR-5232-2022; CBC-1744-2022; HPY-3936-2023The expressions of prostaglandin synthesis enzymes and estrogen, progesterone receptors in canine cystic endometrial hyperplasia (CEH) were reported in this manuscript. Uterine tissue samples were collected from bitches with CEH (n=5), CEH-P (Cystic endometrial hyperplasia-Pyometra) (n=5) and healthy-negative control group, CG (n=5). Immunohistochemistry was carried out for the estrogen (ER) and progesterone receptor (PR) detection. Shock-frozen samples were utilized in mRNA extraction and Real-Time PCR was performed. Gene expression of PTGS2/COX2, PTGES, PTGER4, PGFS, PTGFR and PGR were detected higher in the CEH group compared with CG. The PGFS and PTGFR (FP) mRNA expressions were significantly increased in CEH compared with other groups. Expression of progesterone receptor mRNA (PGR) was highest in CEH and statistically different from the CEH-P group (P<0.05). No PR immunostaining was observed. ER staining had been detected in endometrial glands, endometrial stoma and myometrium, however hyperplasic glands in propria mucosa had lower or no ER scores. Based on the results of this study, the high levels of prostaglandin enzymes and low ER scores in CEH could be a preliminary step for the next stages of severe differentiation of endometrium.Publication The effects of hypoxia on the expression of vascular endothelial growth factor in broiler lung fibroblasts(TÜBİTAK, 2015-01-01) Özyiğit, Musa Özgür; Kahraman, Müjdat Müfit; Akkoç, Ahmet; ÖZYİĞİT, MUSA ÖZGÜR; KAHRAMAN, MÜJDAT MÜFİT; AKKOÇ, AHMET; Veteriner Fakültesi; Patoloji Ana Bilim Dalı; 0000-0002-5090-7917; AAH-2873-2021; CYB-9080-2022; DTZ-3578-2022The aim of this study was to investigate the effects of hypoxic stresses on vascular endothelial growth factor (VEGF) in cultured lung fibroblasts of meat-type chickens. Isolated and subcultured primary lung fibroblasts were exposed to hypoxia. The presence of VEGF and the effects of hypoxia on cultured lung fibroblasts and in the culture media were evaluated. Immunocytochemistry using streptavidin-biotin-peroxidase method and ELISA using commercially available primary monoclonal antibodies were used for evaluation in both 6-h and 12-h hypoxic fibroblasts. The level of VEGF was slightly higher in the 6-h hypoxic group (1.22 ng/mL) than in the 6-h control group (1.27 ng/mL) in cell culture media by ELISA. In the 6-h hypoxic group, a correlation between the decrease in immunocytochemical score and increase in medium with VEGF was observed. The presence of VEGF was shown in cultured chicken lung fibroblasts from meat-type chickens. In conclusion, a significant decrease in immunocytochemical staining score was observed along with a slight increase in the amount of VEGF in culture media under hypoxic conditions. The results suggest that VEGF might have a role in edema formation via permeability increase in the lungs of ascitic meat-type chickens.Publication Demonstration of mycobacterium bovis in cattle via molecular and cytohistopathological diagnostic tecniques and comparison of the results(Ankara Univ Press, 2019-01-01) Akdeşir, Ezgi; Özyigit, M. Özgür; ÖZYİĞİT, MUSA ÖZGÜR; Kahraman, M. Müfit; KAHRAMAN, MÜJDAT MÜFİT; Veteriner Fakültesi; Patoloji Ana Bilim Dalı; AAR-6478-2021In this study the detection of Mycobacterium bovis with classical and molecular techniques and comparisons of the test results in the thirty slaughtered cattle positive to intradermal tuberculin test (PPD) was aimed. The classical methods include macroscopic examination of the organs, microscopic examination of tissues and blood samples that were stained by HE, ZN, immunohistochemistry and immunocytochemistry and PCR as molecular investigation. Characteristic tubercle development was observed in 26 animals' macroscopic examination yet 27 and 20 cases were found compatible with tuberculosis in HE and ZN respectively. Tuberculosis positive results were also obtained from lungs, mediastinal lymph nodes and liver via immunohistochemistry (IHC), PCRtissue, immunocytochemistry of the buffy coat (ICCbc) and PCR of the buffy coat (PCRbc) in 26, 18, 3 and 14 cows respectively. The agents were observed extracytoplasmically and intracytoplasmically in macrophages, giant cells, lymphocytes and fibrocytes in IHC. ICCbc positivity was observed within the cytoplasm of mononuclear leukocytes. Furthermore, the agent was not always detected via ZN staining, which verifies the necessity of ancillary tests. Among these methods IHC has revealed significant sensitivity compared to those of PCRbc and ICCbc (Mc nemar; p<0.05 ve 0.01 respectively), and exhibited compatibility with HE and ZN (Cohen kappa; p<0.001 and 0.05 respectively). These results confirm the fact that rather than one single golden technique, combination of various techniques is necessary for accurate and rapid diagnosis of bovine tuberculosis. It has also been concluded that further investigation is necessary to clarify the efficient routine usage of molecular techniques (PCRtissue/bc) in place of other diagnostic techniques.Publication Evaluation of some systemic inflammatory biomarkers in canine malignant mammary tumors [i](Kafkas Univ, Veteriner Fakultesi Dergisi, 2023-09-01) Koca, Davut; Ekici, Zeynep Merve; Özyiğit, Musa Özgür; ÖZYİĞİT, MUSA ÖZGÜR; Nak, Deniz; NAK, DENİZ; Avcı Küpeli, Zehra; Kuruoğlu, Fikriye Ecem; Avcılar, Talha; Gümüş, Oğulcan; Nak, Yavuz; NAK, YAVUZ; Tıp Fakültesi; 0000-0002-6715-8529; 0000-0002-7962-6959; 0000-0001-7034-9644The aim of this study is to investigate whether neutrophil-to-lymphocyte ratio (NLR), lymphocyte-to-monocyte ratio (LMR), platelet-to-lymphocyte ratio (PLR), systemic immune-inflammation index (SII), albumin-to-globulin ratio (AGR), and prognostic nutritional index (PNI) parameters could be used as biomarkers for canine malignant mammary tumors (MMTs), and the changes in these parameters according to different tumor (T), lymph node (N), and metastasis (M) stages (TNM I-II-III, TNM IV, TNM V) and the number of affected mammary glands (single, multiple). Thirty-seven with MMT and 20 healthy dogs were used in this study. Complete blood count and biochemistry analysis were performed in all dogs. Tumor material is removed by tru-cut and sent to the pathology laboratory for diagnosis. NLR, PLR, and SII values increased, and LMR and PNI values decreased in dogs with MMT. Median NLR values increased and median LMR and PNI values decreased as the TNM stage progressed. In dogs with a single MMT, median NLR, and PLR values were found to be lower than in dogs with multiple MMTs, and median LMR, SII, AGR, and PNI values were higher. The present results indicated that NLR, LMR, PLR, SII, and PNI parameters could be used as biomarkers for canine MMT. Also, NLR, LMR, PLR, SII, PNI, and AGR parameters may be valuable biomarkers that reveal the degree of systemic immune response according to different TNM stages and the number of affected mammary glands.Publication First isolation and molecular characterization of pseudorabies virus detected in Turkey(Springer, 2022-01-15) AYTOĞU, GİZEM; YAVAŞ, ÖZKAN; KADİROĞLU, BERFİN; TOKER, EDA BALDAN; YEŞİLBAĞ, KADİR; Ateş, Özer; ÖZYİĞİT, MUSA ÖZGÜR; Veteriner Fakültesi; Viroloji Ana Bilim Dalı; 0000-0003-2468-3945; 0000-0001-7676-9033; KHD-4075-2024; AAH-3493-2021; ABE-9974-2020Background Pigs are the main host species for the pseudorabies virus. It causes fatal encephalitis in many species, including humans. This article aims to report the first clinical case of pseudorabies as well as isolation and molecular characterization of the virus from a hunting dog in Bursa province, Turkey. Methods and results The dog shows clinical signs including pruritus and neurological signs such as stumbling and inability to stand up compatible with pseudorabies. The virus isolates were obtained from the supernatant of fresh tissue samples from the cerebellum, cornu ammonis, spleen, salivary gland, conjunctival swab, serum, and PBMC samples. The glycoprotein C region is targeted for viral DNA amplification. Pseudorabies virus genome detected both in fresh tissues and supernatants of third passage on Vero cells. The number of PCR positive samples was dramatically increased after cell culture inoculations. Genome sequencing of strain Bursa-10303, which was isolated from a non-endemic area, identified it to belong to clade A. Conclusions This study confirms the possible presence of pseudorabies infection in the wildlife reservoirs in Turkey. Future studies may clarify the importance of the infection in Turkey region, where there is no prevalent pig production.Publication Pharmacologic overview of systemic chlorogenic acid therapy on experimental wound healing(Springer, 2014-11-01) Bağdaş, Deniz; Gül, Nihal Yaşar; Topal, Ayşe; Taş, Sibel; Özyiğit, Musa Özgür; CinkIlıç, NilÜfer; Gül, Zülfiye; Etoz, Betül Cam; Ziyanok, Sedef; İnan, Sevda; Turacozen, Özge; Gürün, Mine Sibel; Bağdaş, Deniz; GÜL SATAR, NİHAL YAŞAR; TOPAL, AYŞE; TAŞ, SİBEL; ÖZYİĞİT, MUSA ÖZGÜR; CinkIlıç, Nilüfer; Gül, Zülfiye; Etoz, Betül Cam; ZİYANOK DEMİRTAŞ, SEDEF; İnan, Sevda; Turacözen, Özge; GÜRÜN, MİNE SİBEL; Fen Edebiyat Fakültesi; Farmakoloji Ana Bilim Dalı; 0000-0001-6225-774X; 0000-0002-3595-6286; 0000-0002-8872-0074; 0000-0003-3878-3808; 0000-0001-8138-5851; AAG-8716-2019; AAH-4272-2021; AAF-9939-2020; ABE-6873-2020; JBJ-7162-2023; AAH-5296-2021; AAR-6478-2021; AAH-2873-2021; E-3364-2018; EOB-5882-2022; GGO-6894-2022; AAH-5296-2021Chlorogenic acid (CGA) is a well-known natural antioxidant in human diet. To understand the effects of CGA on wound healing by enhancing antioxidant defense in the body, the present study sought to investigate the potential role of systemic CGA therapy on wound healing and oxidative stress markers of the skin. We also aimed to understand whether chronic CGA treatment has side effects on pivotal organs or rat bone marrow during therapy. Full-thickness experimental wounds were created on the backs of rats. CGA (25, 50, 100, 200 mg/kg) or vehicle was administered intraperitoneally for 15 days. All rats were sacrificed on the 16th day. Biochemical, histopathological, and immunohistochemical examinations were performed. Possible side effects were also investigated. The results suggested that CGA accelerated wound healing in a dose-dependent manner. CGA enhanced hydroxyproline content, decreased malondialdehyde and nitric oxide levels. and elevated reduced glutathione, superoxide dismutase, and catalase levels in wound tissues. Epithelialization, angiogenesis, fibroblast proliferation, and collagen formation increased by CGA while polymorph nuclear leukocytes infiltration decreased. CGA modulated matrix metalloproteinase-9 and tissue inhibitor-2 expression in biopsies. Otherwise, high dose of CGA increased lipid peroxidation of liver and kidney without affecting the heart and muscle samples. Chronic CGA increased micronuclei formation and induced cytotoxicity in the bone marrow. In conclusion, systemic CGA has beneficial effects in improving wound repair. Antioxidant, free radical scavenger, angiogenesis, and anti-inflammatory effects of CGA may ameliorate wound healing. High dose of CGA may induce side effects. In light of these observations, CGA supplementation or dietary CGA may have benefit on wound healing.Publication Efficacy and toxicity of doxorubicin and cyclophosphamide for the neoadjuvant treatment of locally advanced stage canine mammary tumors(Kafkas Univ, Veteriner Fakultesi Dergisi, 2020-11-01) Shahzad, Abid Hussain; Kuruoglu, Fikriye Ecem; Özyiğit, Musa Özgür; ÖZYİĞİT, MUSA ÖZGÜR; Nak, Deniz; NAK, DENİZ; Küpeli, Zehra Avcı; Ekici, Zeynep Merve; Koca, Davut; KOCA, DAVUT; Avcılar, Talha; Nak, Yavuz; NAK, YAVUZ; Sahin, Mustafa Eren; ; Veteriner Fakültesi; Doğum ve Jinekoloji Ana Bilim Dalı; 0000-0002-6715-8529; 0000-0003-1853-4679; 0000-0002-7962-6959; 0000-0001-7034-9644; 0000-0002-8623-8292; AAH-5494-2021; AAH-2873-2021; AAI-6709-2021; AAR-6478-2021The aim of this study was to investigate the effect and toxicity of a doxorubicin cyclophosphamide combination for neoadjuvant use in dogs with locally advanced mammary tumors. A total of 16 dogs with locally advanced mammary tumors aged 8-14 years was used in this study. Samples were taken from all the dogs via Tru-Cut biopsy and fine needle aspiration biopsy of the lymph nodes prior to neoadjuvant chemotherapy. The samples were sent to the pathology laboratory for histopathological examination. Based on the histopathology results, mammary tumors were diagnosed as adenocarcinoma (n=13), tubulopapillary carcinoma (n=2) and malignant mixed tumors (n=1). For the neoadjuvant chemotherapy, doxorubicin (25-30 mg/m(2)) and cyclophosphamide (100 mg/m(2)) were administered via slow IV injection 4 times at 3-week intervals. Clinical findings such as fever, vomiting, anorexia, lethargy, weight loss, alopecia, enteritis, hematuria, and skin ulceration and laboratory findings such as a decrease in neutropenia and hematocrit values were observed. Based on the longest diameter of the tumors before and after four weeks of chemotherapy, a complete response was detected in 2 dogs (12.5%), a partial response was observed in 9 dogs (56.25%) and the disease was stable in 5 dogs (31.25%). According to results, the toxicity, and efficacy of a doxorubicin and cyclophosphamide combination for neoadjuvant chemotherapy in dogs with locally advanced mammary tumors is acceptable, and the treatment is promising.Publication Cholangiocarcinoma in a spayed female cat(Indian Veterinary Journal, 2003-04-01) Sönmez, Gürsel; Kennerman, Engin; Gölcü, E; Özyigit, Musa Özgür; SÖNMEZ, GÜRSEL; KENNERMAN, ENGİN; Gölcü, E; ÖZYİĞİT, MUSA ÖZGÜR; Veteriner Fakültesi; Dahiliye Bölümü; 0000-0002-2524-3274; AAP-7233-2020; AAR-6478-2021; FDU-5862-2022; CQX-8601-2022