Person: KAÇAR, YİĞİT
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KAÇAR
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YİĞİT
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Publication Evaluation of passive transfer in goat kids with brix refractometer and comparison with other semiquantitative tests(Tübitak Bilimsel ve Teknolojik Araştırma Kurumu, 2019-01-01) Batmaz, Hasan; Kaçar, Yiğit; Topal, Onur; Mecitoglu, Zafer; Gümüşsoy, Kadir Semih; Kaya, Feyyaz; BATMAZ, HASAN; KAÇAR, YİĞİT; TOPAL, ONUR; Kaya, Feyyaz; Veteriner Fakültesi; İç Hastalıkları Ana Bilim Dalı; 0000-0002-1933-7354; 0000-0001-8820-1509; 0000-0003-1991-8957; 0000-0002-8389-4833; 0000-0002-5557-121X; AAH-1712-2021; AAH-4972-2021; IXN-7700-2023; AAH-1677-2021; S-8278-2017The aim of this study was to evaluate the passive transfer (PT) status of goat kids by Brix refractometry and compare the results with other semiquantitative tests (total protein - TP, glutaraldehyde coagulation test - GCT, and gamma-glutamyltransferase - GGT) and immunoglobulin G (IgG). The study was conducted on 75 goat kids born from 47 Saanen goats. The blood samples were collected from the kids on day 0 (presuckling) and on the 1st, 2nd, and 3rd days after birth. The Brix% and TP concentrations were measured with refractometers, and GGT activity was measured using a dry chemistry system. The duration of the GCT was determined in the first 60 min. The serum IgG concentration was measured by goat IgG ELISA kit. On the 1st and 2nd days, serum Brix% in the kids was measured as 9.33 +/- 0.17% and 9.17 +/- 0.14%, respectively. In the 1st and 2nd day serum samples of the kids, IgG was 817.76 +/- 37.34 mg/dL and 1173.29 +/- 47.81 mg/dL, respectively, and GGT was 1298.07 +/- 133.29 IU/L and 692.26 +/- 79.86 IU/L, respectively. The Brix refractometer was found to be more sensitive for detection of PT status in kids on the first and second days after birth, such as TP and GCT, whereas GGT, as an early indicator of PT, was useful only on the first day after birth. We conclude that the Brix refractometer could be used to determine the PT status in goat kids and Brix measurements lower than 8.6%, 9.2%, and 9.3% indicate failure of PT in 1-, 2-, and 3-day-old kids, respectively.Publication Evaluation of the differences in proteomics of high-quality bovine colostrum and low-quality bovine colostrum(Wiley, 2023-09-19) Kaçar, Yiğit; Batmaz, Hasan; KAÇAR, YİĞİT; BATMAZ, HASAN; Veteriner Fakültesi; Dahiliye Kliniği; AAH-4972-2021; HXD-1722-2023BackgroundAlthough there are studies on colostrum and milk proteomics of different species in the literature, there is no published report about different quality bovine colostrums' proteomics.ObjectivesThe aim of this study was to compare the proteome content of high- and low-quality bovine colostrums for the first time.MethodsColostrum samples were collected from 32 Holstein cows from the same farm that had just calved. Brix% levels of colostrums were measured, and then, those with a Brix% value of & GE;27% were classified as high-quality and those with a Brix% value of <22% as low-quality. Three samples from high-quality and low-quality colostrums were selected and proteomic analyses were performed by pooling separately.ResultsTotally 95 proteins were identified in the colostrums, and 19 of them showed significant changes between high- and low-quality colostrums. Expressions in colostrum of glycosylation-dependent cell adhesion molecule-1, cofilin-1, alpha-S2-casein, alpha-lactalbumin, alpha-1B-glycoprotein, actin_cytoplasmic-1, nucleobindin-1, cathelicidin-4, inter-alpha-trypsin inhibitor heavy chain H4, chitinase-3-like protein 1 and monocyte differentiation antigen CD14 were lower, whereas tetranectin, secreted frizzled-related protein-1 (SFRP1), perilipin-2, coatomer subunit epsilon (COPE), butyrophilin subfamily 1 member A1, polyubiquitin-B, lactadherin and albumin levels were higher in high-quality colostrum than low-quality colostrum. Moreover, SFRP1, COPE and cathelicidin-4 proteins were identified for the first time in bovine colostrum. In high-quality colostrum, the most prominently down-regulated proteins were cathelicidin-4 (26.01-fold) and cofilin-1 (17.42-fold), and the most prominently up-regulated proteins were COPE (3.37-fold) and tetranectin (3.07-fold).ConclusionsIt was detected that the proteome contents of high- and low-quality bovine colostrums were different from each other. As new functions are added to the protein databases regarding these proteins detected in colostrums, the interactions of proteins with each other and with other molecules will be detailed and the effects of high-quality colostrums on passive transfer immunity and calf health will be understood in full detail.In the presented study, proteomic differences between high- and low-quality colostrums were investigated for the first time. Totally 95 proteins were identified in the colostrums, and 19 showed significant changes between different quality colostrums. Significantly changing proteins have been found to be involved mainly in biological regulation, organization of cellular components, biogenesis, cellular, developmental, immune system, metabolic, multi-organism, multi-cellular processes, localization, locomotion, stimulus-response and signalling pathways. imagePublication Evaluation of the efficacy of cow colostrum in the treatment and its effect on serum proteomes in calves with cryptosporidiosis(Elsevier, 2022-04-29) Baykal, Ahmet Tarık; Kaçar, Yiğit; AYDIN, LEVENT; KAÇAR, YİĞİT; Aydın, Levent; BATMAZ, HASAN; Batmaz, Hasan; Veteriner Fakültesi; İç Hastalıkları Ana Bilim Dalı; 0000-0002-8814-7351; AAH-4972-2021This study aimed to investigate the effect of using high-quality colostrum in addition to paromomycin on the treatment outcomes and serum proteomes of calves naturally affected by cryptosporidiosis. Thirty Holstein calves infected with only Cryptosporidium spp. were divided into three equal groups. Calves in the PC group received paromomycin orally at a dose of 100 mg/kg once daily for 5 days. Calves in PCOL and PBCOL groups received 250 ml colostrum 3 h after feeding twice a day for 3 days. The PBCOL group was also given 6 g of sodium bicarbonate 15 min before colostrum administration. While the fecal scores of all calves were evaluated daily for 10 days from the initiation of the treatment, fecal oocyst counts were determined on the 0, 1, 2, 3, 5, 7, and 10th days. Brix%, total protein (TP), immunoglobulin G (IgG), gamma-glutamyltransferase (GGT) levels, and proteomic analyses were performed on the 0 and 3rd days' sera. Considering pretreatment values, fecal scores (8th, 2nd, and 2nd day), and fecal oocyst counts (10th, 3rd, and 2nd day) improved in a significantly (p < 0.05) shorter time in the colostrum groups than in the control group. By serum proteomic analysis, 99, 93, and 83 proteomes were detected in PC, PCOL, and PBCOL groups, respectively. Although the significant changes in any protein in Group PC were absent, significant changes were observed in Alpha-1B-glycoprotein (A1BG), Zinc transporterZIP11 (S39AB), Cathelicidin-1 (CTHL1), Actin_ cytoplasmic-1 (ACTB), and Apolipoprotein A-IV (APOA4) proteins in Group PCOL and Alpha-1-antiproteinase (A1AT), Serum amyloid A protein (SAA), Actin-cytoplasmic-2 (ACTG), Protein HP-20 homolog (HP20) proteins in Group PBCOL with colostral treatment, which indicated that the use of colostrum had an effect on calf serum proteomes. The more pronounced healing and shorter clinical improvement time in the colostrum groups especially colostrum with sodium bicarbonate revealed that these proteomes have positive effects in the treatment with their systemic and local effects in the intestines.Publication Comparing the effect of ammonium molybdate versus ammonium molybdate and menbutone on hepatic functions of sheep with subclinical copper poisoning(Wiley, 2022-09-27) Kaçar, Yiğit; Mecitoğlu, Zafer; Batmaz, Hasan; KAÇAR, YİĞİT; MECİTOĞLU, ZAFER; BATMAZ, HASAN; Veteriner Fakültesi; İç Hastalıkları Ana Bilim Dalı; AAH-4972-2021; FQB-3477-2022; HXD-1722-2023This study aimed to investigate the effect of using menbutone in addition to ammonium molybdate on liver enzymes in sheep naturally poisoned with copper. Merino lambs (n = 30), naturally poisoned with copper and which also had high liver enzyme levels, were divided into two groups, each with 15 lambs. The AM + MEN group received ammonium molybdate and menbutone and the AM group received only ammonium molybdate solution. Both groups received 1.7% ammonium molybdate solution (1 mL per 10 kg body weight [BW]) subcutaneously on 0, 2nd and 4th days of the study. Menbutone (Genabil (R), Boehringer Ingelheim, Germany) was administered intramuscularly at a dose of 10 mg/kg BW on days 0 and 2, in addition to ammonium molybdate in the AM + MEN group. Blood samples were collected on days 0 and 7, and aspartate aminotransferase (AST), gamma-glutamyltranspeptidase (GGT) and creatinine levels were evaluated. Over 7 days, AST levels decreased from 351.04 +/- 63.50 IU/L to 286.40 +/- 55.68 IU/L in the AM group (P > 0.05) and from 425.00 +/- 119.25 IU/L to 240.83 +/- 29.62 IU/L in the AM + MEN group (P <= 0.05). GGT levels decreased from 121.16 +/- 15.88 IU/L to 110.39 +/- 10.13 IU/L in the AM group (P > 0.05) and 124.52 +/- 15.50 to 98.60 +/- 9.08 IU/L in the AM + MEN group (P <= 0.05). Based on these findings, the use of menbutone, in addition to ammonium molybdate, has significantly reduced the level of liver enzymes.Publication Evaluation of passive transfer in goat kids with Brix refractometer and comparison with other semiquantitative tests(TÜBİTAK, 2019-01-01) Batmaz, Hasan; Kaçar, Yiğit; Topal, Onur; Mecitoğlu, Zafer; Gümüşsoy, Kadir Semih; Kaya, Feyyaz; BATMAZ, HASAN; KAÇAR, YİĞİT; TOPAL, ONUR; MECİTOĞLU, ZAFER; Kaya, Feyyaz; Veteriner Fakültesi; İç Hastalıkları Ana Bilim Dalı; 0000-0002-1933-7354; 0000-0001-8820-1509; AAH-4972-2021; AAH-1712-2021; IXN-7700-2023; AAH-1677-2021; S-8278-2017The aim of this study was to evaluate the passive transfer (PT) status of goat kids by Brix refractometry and compare the results with other semiquantitative tests (total protein - TP, glutaraldehyde coagulation test - GCT, and gamma-glutamyltransferase - GGT) and immunoglobulin G (IgG). The study was conducted on 75 goat kids born from 47 Saanen goats. The blood samples were collected from the kids on day 0 (presuckling) and on the 1st, 2nd, and 3rd days after birth. The Brix% and TP concentrations were measured with refractometers, and GGT activity was measured using a dry chemistry system. The duration of the GCT was determined in the first 60 min. The serum IgG concentration was measured by goat IgG ELISA kit. On the 1st and 2nd days, serum Brix% in the kids was measured as 9.33 +/- 0.17% and 9.17 +/- 0.14%, respectively. In the 1st and 2nd day serum samples of the kids, IgG was 817.76 +/- 37.34 mg/dL and 1173.29 +/- 47.81 mg/dL, respectively, and GGT was 1298.07 +/- 133.29 IU/L and 692.26 +/- 79.86 IU/L, respectively. The Brix refractometer was found to be more sensitive for detection of PT status in kids on the first and second days after birth, such as TP and GCT, whereas GGT, as an early indicator of PT, was useful only on the first day after birth. We conclude that the Brix refractometer could be used to determine the PT status in goat kids and Brix measurements lower than 8.6%, 9.2%, and 9.3% indicate failure of PT in 1-, 2-, and 3-day-old kids, respectively.