Person: AKKOÇ, AHMET
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AKKOÇ
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AHMET
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Publication Evaluation of some blood parameters and percentage of CD4+or CD8+T cells from spleen and liver from the experimental autoimmune encephalomyelitis mouse model(Wiley, 2021-08-01) Arslan, Gözde; Karacay, Mehmet; Bayram, Gökçen Güvenç; Özoğlu, Efe; Dombaz, Fatma; Etgü, Onur; Yumuşak, Ezgi; Ermiş, Diğdem Yöyen; Akkoç, Ahmet; Yalçın, Murat; Oral, Haluk Barbaros; Arslan, Gözde; Karaçay, Mehmet; ÖZOĞLU, EFE; Dombaz, Fatma; Etgü, Onur; Yumuşak, Ezgi; YÖYEN ERMİŞ, DİĞDEM; AKKOÇ, AHMET; YALÇIN, MURAT; ORAL, HALUK BARBAROS; Sağlık Bilimleri Enstitüsü; Patoloji Ana Bilim Dalı; 0000-0001-7288-3250; 0000-0002-5090-7917; 0000-0002-5600-8162; 0000-0003-0463-6818; K-7285-2012; AAG-6956-2021; JFS-2013-2023; HKW-7185-2023; CXY-4200-2022; FSU-7707-2022; DWR-5356-2022; JIJ-1849-2023; GYL-2038-2022; DTZ-3578-2022Publication The effects of hypoxia on the expression of vascular endothelial growth factor in broiler lung fibroblasts(TÜBİTAK, 2015-01-01) Özyiğit, Musa Özgür; Kahraman, Müjdat Müfit; Akkoç, Ahmet; ÖZYİĞİT, MUSA ÖZGÜR; KAHRAMAN, MÜJDAT MÜFİT; AKKOÇ, AHMET; Veteriner Fakültesi; Patoloji Ana Bilim Dalı; 0000-0002-5090-7917; AAH-2873-2021; CYB-9080-2022; DTZ-3578-2022The aim of this study was to investigate the effects of hypoxic stresses on vascular endothelial growth factor (VEGF) in cultured lung fibroblasts of meat-type chickens. Isolated and subcultured primary lung fibroblasts were exposed to hypoxia. The presence of VEGF and the effects of hypoxia on cultured lung fibroblasts and in the culture media were evaluated. Immunocytochemistry using streptavidin-biotin-peroxidase method and ELISA using commercially available primary monoclonal antibodies were used for evaluation in both 6-h and 12-h hypoxic fibroblasts. The level of VEGF was slightly higher in the 6-h hypoxic group (1.22 ng/mL) than in the 6-h control group (1.27 ng/mL) in cell culture media by ELISA. In the 6-h hypoxic group, a correlation between the decrease in immunocytochemical score and increase in medium with VEGF was observed. The presence of VEGF was shown in cultured chicken lung fibroblasts from meat-type chickens. In conclusion, a significant decrease in immunocytochemical staining score was observed along with a slight increase in the amount of VEGF in culture media under hypoxic conditions. The results suggest that VEGF might have a role in edema formation via permeability increase in the lungs of ascitic meat-type chickens.Publication Isolation and culture of chicken embryonic synovial fibroblasts(Ist Zooprofilattico Sperimentale Abruzzo & Molise G Caporale-izs A&m, 2023-01-01) Dayı, Belma; Sevimli, Alper; Akkoç, Ahmet; AKKOÇ, AHMET; Yumuşak, Ezgi; Veteriner Fakültesi; Patoloji Ana Bilim Dalı; 0000-0002-7773-3419Cells obtained from chicken embryos are often preferred for in vitro studies. These cells, which easily adapt to rapid and continuous growth in the appropriate cell culture environment, are thought to be one of the effective methods in the investigation of leg diseases that are frequently observed in poultry. Leg diseases, especially affecting the joints in chickens, cause locomotor problems and adversely affect animal welfare. In addition, they cause significant health problems and increase mortality. It is known that synovial fibroblasts play an important role in joint diseases. In this study, chicken embryonic synovial fibroblasts were isolated from tissue explants taken from the tibio-metatarsal joint region of brown layer chicken embryos. Characterization of cells was evaluated by immunocytochemistry and hemacolor staining. chicken embryonic synovial fibroblasts showed a strong positive reaction to the vimentin antibody. As a result of hemacolor staining, it was noted that the cell morphology was spindle-shaped. The absence of macrophages in chicken embryonic synovial fibroblast culture was confirmed by the carbon powder uptake. In this present study, we aim to present a useful cell culture protocol such as primary culture, passage, and characterization suitable for chicken embryonic synovial fibroblast to be used in the new scientific research.Publication The histopathological and molecular diagnosis of myocardial sarcocystosis in sheep, and the immunohistochemical correlation of parasitic lesions with mmp-2 and mmp-9(Univ Zagreb Vet Faculty, 2022-01-01) Yilmaz, Rahsan; Ayan, Adnan; Yumusak, Nihat; Akkoc, Ahmet; AKKOÇ, AHMET; Veteriner Fakültesi; Patoloji Ana Bilim Dalı; DTZ-3578-2022This study was aimed at the histopathological and immunohistochemical level investigate cardiac tissue lesions caused by Sarcocystis spp. in sheep, and thereby, to collect detailed information on the presence of these parasites and the pathogenesis of sarcocystosis in ovine animals. PCR, a molecular diagnostic method, was used for the identification of the parasite species. Microscopic examination revealed that out of the 45 ovine cardiac tissue samples examined 23 contained Sarcocystis spp. cysts. The PCR analysis results showed that out of the 23 cardiac tissue specimens infected with sarcocysts, 7 were determined to be infected with Sarcocystis gigantea and 16 with Sarcocystis tenella. Immunohistochemical examination demonstrated that, semiquantitatively, MMP -9 staining was stronger than MMP -2 staining in the periphery of the parasite cysts.Publication Mastocytoma on the tail of a cow(British Cattle Veterinary Assoc, 2008-03-01) Çeçen, G.; Akkoç, A.; Kabakaya, G. U.; Görgül, O. S.; AKKOÇ, AHMET; Çeçen, Gökşen ; Kabakaya, Gülsüm Ülke; Görgül, Osman Sacit; Veteriner Fakültesi; Cerrahi Ana Bilim Dalı; 0000-0001-8537-0761; 0000-0002-5090-7917; 0000-0002-4542-315X; AAK-9543-2020; H-7709-2013; DTZ-3578-2022; CRG-1499-2022Mastocytoma was diagnosed in a six year old Holstein cow. The mass had been enlarging for six months. The enlarging mass was clinically determined to be metastatic to the both subiliac lymph nodes. Cytological examination of both sites revealed mast cell neoplasia.Publication A new protocol for collagen-induced local arthritis model in balb/c mice(Turkish Soc Immunology, 2018-01-01) Güvenç, Gökçen; Karacay, Mehmet; Çiftçi, Kübra; Akkoç, Ahmet; AKKOÇ, AHMET; Oral, Haluk Barbaros; ORAL, HALUK BARBAROS; Yalçın, Murat; YALÇIN, MURAT; Veteriner Fakültesi; Patoloji Ana Bilim Dalı; 0000-0002-1413-3651; 0000-0002-5090-7917; 0000-0003-0463-6818; 0000-0002-5600-8162; AAR-6815-2021; K-7285-2012; AAG-6956-2021Introduction: Numerous models have been described to study the pathogenesis of rheumatoid arthritis, and to develop new therapies; but each of these models has their own limitations. Nowadays, the collagen-induced arthritis (CIA) mouse model is the most commonly used model for rheumatoid arthritis studies in certain mouse strain like DBA/1 mouse. This study aimed to describe a new protocol for local induction of arthritis in Balb/c mice, including the monitoring of clinical arthritis and the protocols for histological examination of paws of mice.Materials and Methods: For the induction of local arthritis in 40 Balb/c mice, they were immunized intra-articularly with type II collagen and subcutaneous complete Freund's adjuvant in 0 day. As the boost immunization, the animals had same injections on day 14. Mice were divided 4 groups, and they were clinically and histopathologically examined for arthritis on 0, 14, 21 and 30 days of arthritis induction.Results: The first signs ol local arthritis appeared in this model 1 week after boost immunization (day 21). The CIA induced paw clinically showed severe erythema and swelling all around the hindquarter on day 21 and 30. The paw reached almost 4 times thicker than the other paws and histopathological examination confirmed the clinical arthritis on day 21 and 30.Conclusion: Using the protocol described, the investigators may reproducibly and economically induce a high incidence of local CIA in Balb/c mice. The described local CLA model may be used to unravel pathophysiological or immunological mechanisms of arthritis, and can also be used to study the effect of new therapeutics.Publication PFKFB2 regulates glycolysis and proliferation in pancreatic cancer cells(Springer, 2020-05-15) Özcan, Selahattin C.; Sarıoğlu, Aybike; Altunok, Tuğba H.; Akkoç, Ahmet; Güzel, Saime; Güler, Sabire; Imbert-Fernandez, Yoannis; Muchut, Robertino J.; Iglesias, Alberto A.; Gürpınar, Yunus; Clem, Amy L.; Chesney, Jason A.; Yalçın, Abdullah; Sarıoğlu, Aybike; Altunok, Tuğba H.; AKKOÇ, AHMET; GÜZEL, SAİME; GÜLER, SABİRE; Gürpınar, Yunus; YALÇIN, ABDULLAH; Veteriner Fakültesi; Biyokimya Ana Bilim Dalı; 0000-0002-8287-6617; 0000-0003-1263-3799; 0000-0003-0796-5000; 0000-0002-7698-0872; 0000-0001-8519-8375; S-2474-2018; GCY-0775-2022; DTZ-3578-2022; AAH-4275-2021; HNI-3945-2023; ABI-4164-2020Tumor cells increase glucose metabolism through glycolysis and pentose phosphate pathways to meet the bioenergetic and biosynthetic demands of rapid cell proliferation. The family of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatases (PFKFB1-4) are key regulators of glucose metabolism via their synthesis of fructose-2,6-bisphosphate (F2,6BP), a potent activator of glycolysis. Previous studies have reported the co-expression of PFKFB isozymes, as well as the mRNA splice variants of particular PFKFB isozymes, suggesting non-redundant functions. Majority of the evidence demonstrating a requirement for PFKFB activity in increased glycolysis and oncogenic properties in tumor cells comes from studies on PFKFB3 and PFKFB4 isozymes. In this study, we show that the PFKFB2 isozyme is expressed in tumor cell lines of various origin, overexpressed and localizes to the nucleus in pancreatic adenocarcinoma, relative to normal pancreatic tissue. We then demonstrate the differential intracellular localization of two PFKFB2 mRNA splice variants and that, when ectopically expressed, cytoplasmically localized mRNA splice variant causes a greater increase in F2,6BP which coincides with an increased glucose uptake, as compared with the mRNA splice variant localizing to the nucleus. We then show that PFKFB2 expression is required for steady-state F2,6BP levels, glycolytic activity, and proliferation of pancreatic adenocarcinoma cells. In conclusion, this study may provide a rationale for detailed investigation of PFKFB2's requirement for the glycolytic and oncogenic phenotype of pancreatic adenocarcinoma cells.Publication Tarantula cubensis extract (Theranekron®) inhibits inflammation in carrageenan-induced acute paw edema in rats(Arquivo Brasileiro Medicina Veterinaria Zootecnia, 2022-01-01) İpek, Volkan; Özfırat, E. C.; ÖZFIRAT, NEVZAT; Topal, A.; Satar, N. Y. G.; GÜL SATAR, NİHAL YAŞAR; Pamukçular, O.; Uçkan, E. M.; Akkoç A.; AKKOÇ, AHMET; Veteriner Fakültesi; 0000-0002-5090-7917; 0000-0001-5874-7797The aim of this study was to investigate the anti-inflammatory effect of alcoholic extract of Tarantula cubensis alcoholic extract (TCAE) in experimentally induced inflammation in rats. Fifty-four adult Sprague-Dawley male rats were randomly divided into nine groups. Paw edema was induced by 0.2mL subplantar (s.p.) injection of 1% carrageenan (CAR) into the right hind paw. Rats were treated with the nonsteroidal anti-inflammatory drug (NSAID) indomethacin (INDO) (10mg/kg, p.o.) or TCAE at different doses (1, 10 or 100 mu g/kg) injected s.c. for systemic or s.p. for local anti-inflammatory effect. Saline was used as control. Changes in paw thickness, volume, and weight were calculated as percentages. Formalin-fixed paws were used for histopathological examination. We detected that TCAE applied s.c. at 10 mu g/kg and 100 mu g/kg doses resulted in thinner paw thickness, lower paw volume, and lower paw weights four hours after the induction of inflammation when compared with the INDO group (p<0.05). The paw edema inhibitory effect of TCAE applied at a dose of 10 mu g/kg, s.c. was 68% when compared with the INDO which had an inhibitory effect of 56%. These results were verified with similar histopathological findings. The anti-inflammatory feature of 10 mu g/kg of TCAE given systematically was similar to the effects of INDO. Our results suggest that TCAE has anti-inflammatory effects by reducing edema and decreasing inflammatory reaction. These results may be attributed to the inhibition of the production of proinflammatory mediators. Thus, TCAE may be considered as a potential anti-inflammatory agent for treating acute inflammatory conditions.Publication Immunohistochemical demonstration of mast cells in bovine papillomatous digital dermatitis of holstein-friesian dairy cattle(Hellenic Veterinary Medical Soc, 2023-10-01) Özgüden-Akkoç, C. G.; ÖZGÜDEN AKKOÇ, CANSEL GÜZİN; Akkoç, A.; AKKOÇ, AHMET; Veteriner Fakültesi; Patoloji Ana Bilim Dalı; 0000-0003-0712-0892; F-2822-2017Mast cells (MC) are unique members of immune system; their location and functions are of great importance in health and disease status. This study aims to evaluate MCs distribution and heterogeneity in healthy and digital dermatitis lesions of dairy cattle for the first time. A total of 50 skin samples, 25 healthy and 25 with digital dermatitis (DD) lesions were sampled in Holstein-Friesian dairy cattle. All samples were stained with hematoxy-lin-eosin (H&E) for microscopic examination and also stained with Toluidine blue for MCs demonstration. Epidermal acanthosis and hyperkeratosis with dermal inflammatory cell infiltrations were in consistent with digital dermatitis lesions. Spirochetal agents were successfully demonstrated with Warthin-Starry staining in 22 out of 25 DD samples. Increased number of mast cell was observed in digital dermatitis samples when compared with healthy skin samples. The average number of intact MCs was 4.6 +/- 2 and 9.3 +/- 1 in healthy and digital dermatitis samples, respectively. Two-fold increase in the number of intact MCs in digital dermatitis samples was observed. Degranulated mast cell numbers in tissue sections were also higher in digital dermatitis samples and 4.25-fold increase was recorded in affected skin samples. Immunophenotype of MCs in skin samples were identified by immunohistochemical staining with anti-tryptase and chymase antibodies. Only tryptase positive MCs were observed in healthy and DD samples. In the statistical analysis, differences in the mean intact and degranulated MCs were found to be significant (mean intact: p<0.05, mean degranulated: p<0.01). Our results possibly suggest that MCs may have important roles in the pathogenesis of bovine digital dermatitis.Publication Evaluation of monocytic/granulocytic cells from spleen and liver in the experimental autoimmune encephalomyelitis mouse model(Wiley, 2021-08-01) Karaçay, Mehmet; Arslan, Gözde; Bayram, Gökçen Güvenç; Dombaz, Fatma; Etgü, Onur; Yumuşak, Ezgi; Ermiş, Diğdem Yöyen; Akkoç, Ahmet; Yalçın, Murat; Oral, Haluk Barbaros; Karacay, Mehmet; Arslan, Gozde; Dombaz, Fatma; Etgu, Onur; Yumusak, Ezgi; YÖYEN ERMİŞ, DİĞDEM; AKKOÇ, AHMET; YALÇIN, MURAT; ORAL, HALUK BARBAROS; Sağlık Bilimleri Enstitüsü; Fizyoloji Ana Bilim Dalı; 0000-0001-7288-3250; 0000-0002-5090-7917; 0000-0002-5600-8162; 0000-0003-0463-6818; JFS-2013-2023; K-7285-2012; HKW-7185-2023; AAG-6956-2021; CXY-4200-2022; DWR-5356-2022; CPT-2053-2022; GYL-2038-2022; DTZ-3578-2022