Person: ZIK, BERRİN
Loading...
Email Address
Birth Date
Research Projects
Organizational Units
Job Title
Last Name
ZIK
First Name
BERRİN
Name
3 results
Search Results
Now showing 1 - 3 of 3
Publication The effect of capsaicin on IGF-I and IGF-IR expression in ovarian granulosa cells(Hellenic Veterinary Medical Soc, 2020-01-01) Güler, Sabire; Zik, Berrin; Güler, Sabire; ZIK, BERRİN; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı; AAH-9810-2021; HTY-9355-2023Capsaicin (trans-8-methyl-N-vanillyl-6-noneadamide) is a pungent ingredient in red peppers from the Capsicum family. Insulin-like growth factor-I (IGF-I) is expressed in granulosa cells and has an important role in ovarian development. However, there are no data about the IGF-I expression in ovarian granulosa cells after capsaicin treatment. The aim of this study was to investigate the expression of IGF-I and its receptor (insulin-like growth factor-I receptor [IGF-IR]) in primary rat ovarian granulosa cells after low and high doses of capsaicin treatment. For this, granulosa cells were isolated and cultured from ovaries of 30-day-old female Sprague-Dawley rats. Granulosa cell plates were divided into four groups as cell control (C), vehicle control (V), and 50 mu M and 150 mu M capsaicin groups. In experimental groups, granulosa cells were exposed to capsaicin for 24 hours and immunocytochemistry was performed afterwards using anti-IGF-I and anti-IGF-IR antibodies. Both IGF-I and IGF-IR expressions were found to be significantly increased in parallel to the capsaicin doses. Elevated levels of IGF-I may be a risk factor for ovarian development. Because of the crucial role of IGF-I in ovary development, capsaicin treatment can be effective on follicular development and/or disorders characterized by high IGF-I levels.Publication Overexpression of dual-specificity phosphatases 4 and 13 attenuates transforming growth factor β1-induced migration and drug resistance in A549 cells in vitro(Academic Press Inc Elsevier Science, 2022-03-23) Güler, Sabire; Altunok, Tuğba H.; Sarıoğlu, Aybike; Zik, Berrin; Aşmaz, Deniz; Kayapunar, Nuray; Sönmez, Öner; Tepedelen, Burcu Erbaykent; Yalçın, Abdullah; GÜLER, SABİRE; Altunok, Tuğba H.; Sarıoğlu, Aybike; ZIK, BERRİN; Aşmaz, Deniz; Kayapunar, Nuray; SÖNMEZ, ÖNER; Tepedelen, Burcu Erbaykent; YALÇIN, ABDULLAH; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Biyokimya Anabilim Dalı.; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Moleküler Biyoloji ve Genetik Anabilim Dalı.; 0000-0002-7367-6859; 0000-0003-1263-3799; 0000-0002-1062-332X; 0000-0001-8519-8375; 0000-0002-8287-6617; 0000-0001-6468-8535; AAH-8807-2021; KMY-2643-2024; S-2474-2018; AAH-9810-2021; HPG-0648-2023; GXM-5514-2022; DTN-7054-2022; AAH-6436-2021; ABI-4164-2020Transforming growth factor-beta (TGF beta) proteins induce an epithelial-mesenchymal transition (EMT) programme that is associated with increased invasive and drug-resistant phenotype of carcinoma cells. In addition to the canonical pathway involving SMAD proteins, the mitogen-activated kinase (MAPK) pathway via extracellular signal-regulated kinases 1/2 (ERK1/2) is also involved in promoting and maintaining a mesenchymal phenotype by tumor cells following TGF beta signal activation. As dual-specificity phosphatases (DUSPs) regulate ERK1/2 activity by dephosphorylation, we aimed to examine DUSPs' expression upon TGF beta stimulation and whether DUSPs play a role in the EMT and related phenotypes promoted by TGF beta 1 in A549 cells. We found that TGF beta 1 stimulation led to marked changes in several DUSP proteins, including significant decreases in DUSP4 and DUSP13 expressions. We then showed that the ectopic co-expression of DUSP4/13 suppresses TGF beta 1-induced ERK1/2 phosphorylation and protein levels of the EMT transcription factors Snail and Slug proteins. We then demonstrated that DUSP4/13 co-expression partially inhibited TGF beta 1-promoted migration, invasion, and chemoresistance in A549 cells. Collectively, this report provides data for the involvement of DUSP4/13 in malignant phenotypes regulated by TGF beta 1 in A549 cells. (C) 2022 Elsevier Inc. All rights reserved.Publication Synergistic effect of feed additives on cell proliferation and morphology in quail (coturnix coturnix japonica) duodenum(Hellenic Veterinary Medical Soc, 2022-07-01) YEŞİLBAĞ, DERYA; Asmaz, Ender Deniz; Yeşilbağ, Derya; Odabası, F.; Zık, Berrin; ZIK, BERRİN; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; AAH-9810-2021; HPG-0648-2023The present study aimed to investigate the effect of a commercial probiotic and a commercial essential oil blend and their mixture, as a natural feed additive, on the expression level of Proliferating Cell Nuclear Antigen (PCNA), proliferation index (PI), and total mucosal thickness of quails. 1-day-old Japanese (Coturnix coturnix Japon-ica) quails, including both males and females, were divided into four groups as follows: (1) control treatment without medication (n: 15); (2) 18 g/ ton-1 probiotic (n: 15); (3) 300 g/ ton-1 essential oil blend (n: 15) and (4) 18 g/ ton-1 probiotic plus 300 g/ ton-1 essential oil blend (n: 15). The study evaluated the effect of natural feed additives added to the quail diet on intestinal health by immunohistochemical and histological analysis. At the end of the experiment, the quail's duodenum was removed, fixed in 10% neutral buffered formalin, and performed a histological examination.As a result of the study, it was determined that the PCNA expressions, proliferation index, and mucosal thickness were generally significantly increased in the experimental groups compared to the control group (P<0.001). The combina-tion of additives caused a synergistic effect for this study. All data were statistically significant in the group where the probiotic and essential oil mixture was used together. It has been determined that these feed additives combination stimulates cell proliferation in the duodenum, where nutrients are absorbed and positively affect intestinal morphology.