Uluslararası Bilim, Teknoloji ve Tasarım Dergisi

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    Development of alternative methods for determination of acrylamide and caffeine in coffee samples
    (Bursa Uludağ Üniversitesi, 2020-08-21) Kuruca, Büşra; İzgi, Belgin; Fen Edebiyat Fakültesi; Kimya Bölümü Lisans Öğrencisi; 0000-0002-9177-6992; 0000-0002-1074-3612
    Acrylamide which has gained attention in recent years is a composition resulting from the heat treatment process of foods. By the IARC, it is listed in Group 2A as a possible carcinogenic substance for humans. Caffeine is a nitrogenous natural compound of the alkaloid group, which has crucial physiological effects. There are numerous analytical methods within the literature for acrylamide and caffeine determination. These methods are GC-MS, LC-MS, voltammetry, ion chromatography, FTIR, GC-EDC, LC- MS/MS, and GC-IT/MS. Also, organic molecules could be identified via the complexation with metals. It is well known that the Job Method is determining the formation rate of the complex between metals and organic molecules in a certain mixture. In this project, an alternative method was tried to determine the CA and AA amount in coffee samples. First of all, the metal complexes of CA and AA formed with Cu and Co were determined as 1:1 according to Job's Method by UV-VIS Spectrophotometer. After optimum conditions were set, standard solutions and coffee samples were measured at 252.0 nm (for CA) and 272.0 nm (for AA). Based on the data obtained, the amounts of acrylamide and caffeine were determined in commercial coffee samples.