2021 Cilt 40 Sayı 1

Permanent URI for this collectionhttps://hdl.handle.net/11452/27898

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The case of gastrointestinal nematodiosis forming a nodule in a gazelle’s (gazella subgutturosa) abomasum
(Bursa Uludağ Üniversitesi, 2021-05-05) Yılmaz, Rahşan
This study has reported the case of abomasal teladorsagiosis in gazelle (Gazella subgutturosa) that was found dead while under care at a wildlife reserve area in Sanliurfa, Turkey. Necropsy was performed on six-year-old, male gazelle, which was found dead in its natural environment and brought to the Department of Pathology, Faculty of Veterinary Medicine, Harran University. At necropsy were observed became whitish in color and thickened the plicae spirals on the abomasum mucosa. In the microscopic examination, parasites were encountered on the mucosa abo masum and lumen of the glands in lamina propria. Also in the lumen of the glands with parasited were detected necrotic debris and neutrophil leukocyte accumulation and to increase in the connective tissue between the glands. In the scannig electron microscopic examination, parasites were observed on the abomasum mucosa. This study is the first report case describing gastrointestinal nematodiosis forming a nodule in the abomasum of in a gazelle in Turkey.
Comparison of growth curve in male layer chickens
(Bursa Uludağ Üniversitesi, 2020-12-24) Gunawan, Andrean Amar; Putra, Widya Pintaka Bayu; Riaz, Roshan; Orman, Abdülkadir; Ziraat Fakültesi; Hayvan Bilimleri Bölümü; 0000-0002-1102-6447; 0000-0002-0524-9994; 0000-0001-9138-4422
This study was aimed to obtain the growth curve of body weight in male layer chickens (Lohman MB 202) based on Logistic and Gompertz models. The frequently data of body weight from one day age to adult age were used for growth curve prediction. Total of one thousand birds from private sector poultry farm in Tasikmalaya Regency, Indonesia were used in this study as the data source. The growth curve estimation was calculated based on Logistic (L) and Gompertz (G) models using Curve Expert 1.4. computer program. The final weight (asymptote) in birds was reached of 1111.27 g (L) and 1685.13 g (G). Therefore, the weight of inflection (Wi) of birds were reached of 555.64 g (L) and 619.53 g (G). The time of inflection (ti) and maximum growth rate (GR) of birds in both models were 6 weeks and 19 g/week respectively. In conclusion, both models had similar coefficient of determination (R2) value. However, the growth curve of Gompertz model was confirmed as better growth curve for body weight of birds than that of Logistic model due to lower of root mean square error (RMSE) value.
Effect of ambient temperature on death rate, reject rate, dressing percentage, and economic loss in broilers during transport to slaughterhouse
(Bursa Uludağ Üniversitesi, 2021-05-12) Çavuşoğlu, Enver; Riaz, Roshan; Veteriner Fakültesi; Hayvan Bilimleri Ana Bilim Dalı; 0000-0002-9018-3511; 0000-0002-0524-9994
The process of transporting broilers from the farm to the slaughterhouse is critical for animal welfare and for the production economy. During the transport process, the animal may suffer and die and this situation negatively affects animal welfare and profitability. The present study aimed to assess the impact of ambient temperature on the death rate, reject rate, and total economic loss in broilers during transportations in Turkey. The data of a total of 1,015,336 broilers transported for a two-year period were collected from a commercial abattoir. The average ambient temperature of the exact days of consignments was divided into three subgroups: Temperature (T), T1 (10.0-14.0 °C), T2 (14.1-19.0 °C), T3 (19.1-24.0 °C). The average death rates were 1.890%, 1.393%, 0.326%, and reject rates were 1.151%, 1.611%, and 1.441% found for T1, T2, and T3, respectively. Analysis of data showed the significant (p<0.001) effect of the ambient temperature on the death rate, and the higher impact was observed for T1 and lower for the T3. The reject rate among all groups was statistically significant (p=0.044), and the highest reject rate was found in T2 while the lowest reject rate was found in T1. The net economic loss due to rejected birds and death were 35,403 US$ and 30,692 US$, respectively, while the total economic loss was 66,095 US$. In conclusion, the results of the present study showed that death rate, reject rates, and dressing percentage are impacted by the ambient temperature. Furthermore, death rates, rejection rates, and potential economic losses of the birds are linked with transportation from farm to the processing plant, and there is a need for management strategies to be refined through research by assessing the on-farm to processing plant risk factors, their linkages, as well as their potential contributions in Turkish integrated broiler production system.
The effect of gender factor on mandibular morphometry in Abaza goats
(Bursa Uludağ Üniversitesi, 2021-07-08) Dalga, Semine; Aslan, Kadir
In In this study, the mandibles of Abaza goats were examined macroanatomically and morphometrically. A total of 14 adult (7 female / 7 male) Abaza goat mandibles of both sexes of different age and of body weight were used. Mandibles was removed from the head and cleaned of skin and rough meat, then macerated with hydrogen peroxide for 25-30 minutes and the lengths of 16 different points were measured by electronic caliper (0.00, BTS, UK) according to the literature. Mean, standard deviation and correlation values of all measurements were determined in SPSS (20.0) version program. As a result of the statistics, the mean length of mandible in females was 158,73 mm and in males 148,72 mm. The mean height was 90.46 mm in females and 88.60 mm in males. Morphometric measurements showed that all lengths except L15 were higher in female Abaza goats. Only a significant difference was found between mandibular height (L16) at the level of the anterior alveolar edge of the second premolar tooth (P<0,01).
Effect of the parity and the production year on the longevity of the kids in saneen dairy goats
(Bursa Uludağ Üniversitesi, 2021-01-22) Çavuşoğlu, Enver; Riaz, Roshan; Omar, Mohamed Yusuf; Demir, Mehmet; Orman, Abdülkadir; Veteriner Fakültesi; Hayvan Bilimleri Ana Bilim Dalı; 0000-0002-9018-3511; 0000-0002-0524-9994; 0000-0002-5481-729X; 0000-0002-9279-3911; 0000-0001-9138-4422
The basic objective of the dairy goat farmers is to produce healthy and fertile animals at appropriate age, with maximum genetic expression and long productive duration. Longevity is an important economical parameter in the breeding program of the dairy goats. There are various factors which directly influence the production and longevity of the newborns such as age at birth, season, herds flock and breed. The present study aimed to study the effect of the parity and the production year on the kids’ longevity in Saanen dairy goats. The 5 year data (2012 to 2016) of total 222 Saanen female goats collected from the Research and Applied Center at the Faculty of Veterinary Medicine, Bursa Uludag University. The effect of parity on the kids’ longevity was found non-significant (P>0.05). Non-significant (P>0.05) difference was observed while comparing the data of kids from primiparous and multiparous within the production year. However, a significant (P<0.05) difference among the mean values was found while making comparison between the production years. The litter size in the present study ranged from 1.57 to 1.75 and non-significant (P>0.05) difference was found in the kids born over the evaluated years. The present study did not investigate the voluntary and involuntary culling reasons and the management practices at the farm are more standardized than a normal commercial farm. Hence, further research is required at the commercial farms having large number of the animals and herds in order to use the parity and longevity in the present genetic evaluation programs.
Genetic variability of FABP4 c.328 G>A (rs110652478) polmorphism and its association with slaugter weight and carcass traits in aberdeen angus and hereford bulls imported into Turkey
(Bursa Uludağ Üniversitesi, 2021-06-09) Ardıçlı, Sena; Üstüner, Hakan; Arslan, Öznur; Veteriner Fakültesi; Genetik Ana Bilim Dalı; 0000-0003-2758-5945; 0000-0002-4341-5842; 0000-0002-4402-2434
The study aimed to determine the genotypic distribution of the c.328 G>A (rs110652478) polymorphism in the bovine fatty acid-binding pro tein 4 (FABP4) gene and to determine its effect on slaughter weight in Aberdeen Angus and Hereford bulls imported into Turkey. A total of randomly selected 95 bulls were used in this study. Bulls were housed for fattening in semi-open pens for approximately nine months with the same management procedures. Genotyping of the FABP4 c.328 G>A (rs110652478) polymorphism, located in exon 3, was performed by the PCR-RFLP method. Allele and genotype frequencies were calculated by the standard procedures and the Hardy-Weinberg equilibrium was tested by comparing expected and observed genotype frequencies using chi-square and Fisher’s exact tests. Indices of genetic diversity were calculated based on the allelic frequencies. The least-squares method as applied in a GLM procedure was used to test the association between FABP4 c.328 G>A and phenotypic traits including slaughter weight, hot and cold carcass weight, chilling loss, dressing percentage, and carcass pH. Results revealed that the most frequent genotype was GG (49.18% and 52.94% for Angus and Herefords, respectively) and the minor allele frequency (A allele) was 0.32 in the total population. The genotype distribution was in agreement with Hardy Weinberg equilibrium (P>0.05). The moderately low levels of genetic variability observed in this study may be caused as a result of eventual inbreeding. PIC levels suggest that FABP4 c.328 G>A can be a mildly informative genetic marker for the tested population. Association analysis indicated that the effect of the FABP4 on slaughter weight was statistically significant (P<0.05) in the total population. However, this association was not substantiated in breed-specific analyzes. There was no significant effect of the marker on any remaining phenotypic traits. Consequently, it is important to emphasize that analysis of FABP4 c.328 G>A may provide valuable, but limited, information on imported beef cattle raised in Turkey conditions.
A geometric and mechanical description of the talus and cochlea tibiae in horse and ox
(Bursa Uludağ Üniversitesi, 2021-06-29) İşbilir, Fatma; Çelik, A. Burcu; Arslan, Kadri; Bulca, Betül; Yıldız, Bahri; Fen Edebiyat Fakültesi; Anatomi Ana Bilim Dalı; 0000-0002-6110-1302; 0000-0002-6948-8281; 0000-0002-1440-7050; 0000-0001-5861-0184; 0000-0002-6096-7833
In this study, geometrical and mechanical identification of the talus and cochlea tibiae in horse and ox is presented. The shape of the expressed bones of these animals can be considered as rotational surfaces of planar curves. The model is established based on the bolt-nut mechanism while interpreting the relationship between talus and tibiae, and describing geometrical differences between ox and horse in functional anatomy. The results obtained from the geometric and mechanical data have led to the conclusion that lateral and friction forces applied on talus and cochlea tibiae of the horse are higher than that in ox. According to these results, the data obtained helps the horses to stand longer.
Investigating burnout levels and its determinants among the veterinary medicine students: The case of Istanbul University
(Bursa Uludağ Üniversitesi, 2021-05-24) Öztürk, Nursen; Kılıç, Halil İbrahim; Ekiz, Bülent
This study was conducted to examine burnout levels and their determinants among veterinary medicine students. Answers (n = 447) to a survey formed the data of this study. Maslach Burnout Inventory - Student Scale, which was adapted to the Turkish language, was used to determine the burnout levels of the students. As a result, burnout levels were obtained as 13.31, 7.82, and 7.69 for Emotional Exhaustion (EE), Depersonalization (DP), and Personal Accomplishment (PA) components, respectively. Year of study and having a hobby affected three of the burnout components. In order to determine the relationship between students’ burnout levels and students’ tendency to drop out, to work in a different profession after graduation and academic failure, a logistic regression analysis was conducted. Results revealed that year of study, weighted grade point average (WGPA), EE, DP, PA, repeating academic semester(s), number of the repeated academic semester(s), and perceived difficulty of veterinary education were significantly associated with the students’ intention to drop out of the faculty. Gender, living situation, monthly income, EE, DP and PA affected education-occupation mismatch. Furthermore, gender, year of study, WGPA, living situation, monthly income, EE, DP, PA, and perceived difficulty level of the veterinary education influenced repeating academic semester(s). The results of this study reveal that burnout syndrome would lead to students’ dropping out of the faculty, education-occupation mismatch and academic failure. Future studies must be conducted throughout the country to assess burnout syndrome in veterinary students.
Liyofilizasyon amaçlı kullanılan sulandırıcıların koç spermasının DNA bütünlüğü üzerine etkisi
(Bursa Uludağ Üniversitesi, 2021-04-27) Birler, Sema; Öztürk, Gül Bakırer; Papuccuoğlu, Serhat; Üstüner, Burcu; Zık, Berrin; Alçay, Selim; Güler, Sabire; Sağırkaya, Hakan; Nur, Zekariya; Veteriner Fakültesi; Histoloji Embriyoloji Ana Bilim Dalı; 0000-0001-5999-4685; 0000-0002-1053-6250; 0000-0002-2472-8157; 0000-0002-7367-6859; 0000-0001-6619-3229; 0000-0002-1438-221X
DNA hasarının belirlenebilmesi, sperma saklama yöntemlerinin geliştirilmesi açısından önemlidir. Biz çalışmamızda farklı liyofilizasyon medyumları kullanılarak saklanan sperm hücrelerinin DNA bütünlüğünün değerlendirilmesinde akridin oranj (AO) ve therminal deoxynucleotidyl transferase-medited dUDP nick and labelling (TUNEL) floresan boyama yöntemlerini kullandık. Koçlardan alınan sperma dört gruba bölündü ve her bir grup: I) %10 fötal buzağı serumu (FCS) içeren TCM 199 solüsyonu II) %10 FCS ve 0,2 mol/L trehaloz içeren TCM 199 solüsyonu III) 50 mmol/L NaCl ve EGTA [Ethylen glycol-bis (β-aminoethyl ether)-N, N, N, N,-tetraacetic acid] 10 mmol/L Tris solüsyonu ve IV) %20 yumurta sarısı ve %7 gliserol içeren Tris bazlı solüsyonlardan biri ile konsantrasyonu 10 x 106 spermatozoa/100 μL olacak şekilde sulandırıldı. Taze alınan spermada DNA fragmentasyon oranı AO ve TUNEL yöntemlerinde sırasıyla %2,0 ±1,2 ve %3,8±3,7 olarak tespit edildi (P>0.05). AO ve TUNEL yöntemiyle değerlendirilen liyofilizasyon sonrası DNA bütünlüğüne sahip spermatozoa oranlarının, sulandırıcı farklılığına göre etkilenmediği tespit edildi (P>0.05). Aynı şekilde her iki boyama yöntemi karşılaştırıldığında, liyofilizasyon sonrası elde edilen DNA bütünlüğünün değerlendirilmesinde AO ve TUNEL sonuçlarının istatistiksel olarak farklı olmadığı belirlendi (P>0.05). Çalışmamızın sonuçları; sulandırıcı gruplarından her birinin koç spermasının liyofilizasyonu için uygun olduğunu ve her iki boyama yönteminin de tercih edilebileceğini göstermektedir.
Sığır karkas ve sakatatlarında hijyen profilinin yasal mevzuat kriterleri ile değerlendirilmesi
(Bursa Uludağ Üniversitesi, 2021-02-19) Çetin, Ece; Ertekin, Ayşegül; Coşkun, Ahmet Gökhan; Temelli, Seran; Eyigör, Ayşegül; Sağlık Bilimleri Enstitüsü; Besin Hijyeni ve Teknolojisi Ana Bilim Dalı; 0000-0002-5121-2631; 0000-0002-5181-7577; 0000-0002-8869-4929; 0000-0002-2707-3117
Çalışmada, kasaplık sığırların karkas ve sakatatlarının Türk Gıda Kodeksi (TGK) Mikrobiyolojik Kriterler Yönetmeliği Üretim Hijyeni Kriter leri gereklilikleri dahilinde mikrobiyolojik kalitesinin belirlenmesi hedeflendi. Bu amaç ile, Marmara Bölgesinde 2013-2015 yılları arasında, 3 kombina ve 1 mezbahadan toplam 400 adet örnek alındı. Alınan 100 adet karkas örneği, Aerobik Koloni Sayısı (AKS) ve Enterobacteriaceae sayısı (ES)’nın belirlenmesi ve Salmonella varlığı yönünden, her bir karkasa ait olmak üzere toplamda 300 adet yenilebilir sakatat (100 ka raciğer, 100 dalak, 100 böbrek) örneği ise Salmonella varlığı açısından, ilgili uluslararası standartlar olan ISO 4833-1:2003 (AKS), ISO 21528- 2:2004 (ES) ve ISO 6579:2002 (Salmonella) kullanılarak analiz edildi. İncelenen karkas örneklerinde AKS, 3,0x102- 4,0x105 kob/cm2 aralığında olup ortalama 3,1 x 104 kob/cm2, ES ise 0,1 x 101- 8,5 x 102 kob/ cm2 aralığında olup ortalama 1,9 x 102 kob/cm2 olarak bulundu. AKS ve ES sonuçlarının birbirinden bağımsız olarak ilgili yönetmeliğe göre değerlendirilmesi sonucunda, örneklerin AKS yönünden; %34’ü ‘Uygun’, %56’sı ‘Kabul Edilir’ ve %10’u ‘Uygun Değil’, ES yönünden de %57’si ‘Uygun’, %34’ü ‘Kabul Edilir’ ve %9’u ‘Uygun Değil’ olduğu tespit edildi. TGK Mikrobiyolojik Kriterler Yönetmeliği Üretim Hijyeni Kriterleri’ne göre AKS ve ES yönünden sırasıyla %90 ve %91, AKS ve ES sonuçları birlikte değerlendirildiğinde ise %84 oranında uygun bulunan sığır karkaslarının, ayrıca sakatatları dahil Salmonella içermediği göz önünde bulundurulduğunda, örnekleme yapılan dönem içinde hijyenik kalitesinin oldukça iyi düzeyde olduğuna karar verildi.
Tulareminin serolojik tanısı için in house enzyme- linked immunosorbent assay (ELISA) prototipi ve mikroaglütinasyon Test (MAT) antijeninin geliştirilmesi
(Bursa Uludağ Üniversitesi, 2021-05-04) Gürbilek, Sevil Erdenliğ; Tel, Osman Yaşar; Yücetepe, Ayfer Güllü; Keskin, Oktay
Bu çalışmada, Francisella tularensis (F. tularensis)’e karşı gelişen antikorları saptamak için bir Enzyme- Linked Immunosorbent Assay (ELI SA) prototipi ve kristal viyole boyalı bir mikroaglütinasyon test (MAT) antijeni geliştirildi. Ayrıca konfirme edici Western blot (WB) tekniği de serumlara uygulandı. Alınan sonuçlara göre MAT, ELISA ve WB testlerinin tanısal duyarlılıkları %100 olarak bulunurken, özgüllükleri sırası ile %100, %98 ve %96 olarak bulundu. Geliştirilen MAT ve ELISA ile 72 insan serumunda seropozitiflik oranı her iki teste de %4.2 olarak bulunurken, 190 koyun serumunda MAT ve ELISA seropozitifliği sırası ile %3.2 ve %4.7 olarak bulundu. Alınan sonuçlara göre ülke mizde tularemi insan ve hayvanlarda varlığını sürdüren bir infeksiyondur. Ancak daha sağlıklı epidemiyolojik yorum yapabilmek için daha çok sayıda seruma ve farklı hayvan türleri ile çalışılmasına ihtiyaç bulunmaktadır. Sonuç olarak, ELISA ve konfirme edici Western blotting kombinasyonunun tulareminin serolojik tanısında kullanılabilecek uygun bir kombinasyon olduğu düşünülmektedir.

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