Person: AYDIN, CENK
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AYDIN
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CENK
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Publication Erythrocyte potassium, sodium and GSH concentrations of Saanen and Turkish hair breeds of goats(Zoological Soc Pakistan, 2008-01-01) Aydın, Cenk; Doğrutekin, Deniz; Udum, Duygu; Çetin, Meltem; AYDIN, CENK; Doğrutekin, Deniz; UDUM, DUYGU; Çetin, Meltem; Veteriner Fakültesi; Fizyoloji Ana Bilim Dalı; 0000-0002-3090-0099; 0000-0001-7052-1694; AAH-5167-2021; DWC-5118-2022; ESX-0896-2022; HQE-4960-2023Biochemical variation of three genetic markers, namely erythrocyte potassium (K-e), erythrocyte sodium (Na-c) and erythrocyte glutathione (GSH) in sixty Saanen and Turkish Hair Goats was analysed. The potassium and sodium concentrations were determined by flame photometry. The level of GSH in erythrocytes was determined by the DTNB method. The frequencies of the mentioned loci have been calculated and compared with some of the goat breeds studied so far by other authors. Our results indicated that there were bimodal distributions in K-e and GSH concentrations in Saanen goat breeds, whereas there was only GSH polymorphism in Turkish Hair Goats. The distribution of K-e concentrations Suggested the presence of high-K/low-K (HK/LK) polymorphism, based on the predetermined dividing value of 50 meq/L of K-e concentration in erythrocytes of Saanen Goats. Both types are shown to be controlled genetically by an autosomal locus with two alleles HK and LK, with HK being dominant over LK. While majority of the Turkish Hair Goat population (93%) had low (GSH(h)) or 'deficient' erythrocyte GSH concentrations (<50 mg/100 mL erythrocyte), Saanen breeds (93%) had high (GSH H) erythrocyte GSH levels. Relationships between the parameters were also evaluated. In Saanen Goats, erythrocyte K-e and Na-e values exhibited positive correlation with the total monovalent cation concentrations (K-e + Na-e) while in Turkish Hair Goats only K-e value showed correlation with the same monovalent cations.Publication Dietary intake of Spirulina platensis alters HSP70 gene expression profiles in the brain of rats in an experimental model of mixed stress(Indian Acad Sciences, 2022-10-26) Seyidoğlu, Nilay; ARDIÇLI, SENA; Ardıçlı, Sena; Gurbanli, Rovshan; Köseli, Eda; Aydin, Cenk; AYDIN, CENK; Veteriner Fakültesi; ; 0000-0003-2758-5945; 0000-0002-4812-4024; 0000-0002-3090-0099; KIC-9651-2024; O-3394-2019; JTT-0803-2023; AAS-2344-2020; GRJ-2026-2022Spirulina platensis has gradually gained more attention for its therapeutic, antioxidant, and anti-inflammatory potential worldwide. However, the current molecular knowledge about the effects of spirulina on stress-related genes is rather limited. The effects of dietary intake of spirulina on the HSP70 gene expression were assessed in a controlled in vivo experimental design. Moreover, alterations in serum corticosterone levels, IL-2, IL-4, IFN-gamma, triglyceride, ALT, AST, relative gene expression values, and the correlations between them were evaluated. A total of 36 rats were divided into four groups: control group, stress-only group, spirulina group, and spirulina+stress group. To control the dose administration, S. platensis was applied by a gastric gavage in stress groups. Crowded environment stress and hosting alone stress were applied to the stress-only group and spirulina + stress group. RNA was extracted from brain samples using TRlpure and the relative gene expression assessment was performed using Roche-LightCycler-480-II real-time PCR-System. Gene expression values were remarkably different among the four experimental groups. The differences between stress-only and the spirulina groups were statistically significant (P<0.05). The correlation between the HSP70 gene expression and the IFN-gamma was found to be statistically significant (P<0.05; r=0.50). Results indicate a novel effect of spirulina on the HSP70 expression related to the stress-response. Data presented in this study may be useful for further studies to define not only the molecular genetic aspects through dietary S. platensis but also the effects of spirulina on stress-response and animal welfare.Publication The preventive role of spirulina platensis (Arthrospira platensis) in immune and oxidative insults in a stress-induced rat model(Walter, 2021-06-01) Seyidoğlu, Nilay; Köşeli, Eda; Gurbanlı, Rovshan; Aydın, Cenk; Köşeli, Eda; Gurbanlı, Rovshan; AYDIN, CENK; Veteriner Fakültesi; Fizyoloji Bölümü; 0000-0002-4812-4024; DWC-5118-2022; CRR-4475-2022; GRJ-2026-2022Introduction: There is a balance between oxidative stress, antioxidant capacity and immune response. Their roles in physiological and behavioural mechanisms are important for the maintenance of the organism's internal equilibrium. This study aimed to evaluate the antioxidant effects of the exogenous alga Spirulina platensis (Arthrospira platensis) in a stress-induced rat model, and to describe its possible mechanism of action. Material and Methods: Thirty-six adult male Sprague Dawley rats were separated into four groups: control (C), stress (S), S. platensis (Sp), and S. platensis + stress (SpS). The rats in groups Sp and SpS were fed with 1,500 mg/kg b.w./day Spirulina platensis for 28 days. All rats were exposed to prolonged light phase conditions (18 h light:6 h dark) for 14 days. The SpS and S groups were exposed to stress by being kept isolated and in a crowded environment. Blood samples were obtained by puncturing the heart on the 28th day. The effect of stress on serum corticosterone, oxidative stress markers (TOS, TAC, PON1, OSI) and immunological parameters (IL-2, IL-4, IFN-gamma) were tested. Also, the brain, heart, intestines (duodenum, ileum, and colon), kidney, liver, spleen, and stomach of the rats were weighed. Results: Serum corticosterone levels were higher in the S group than in the C group, and significantly lower in the SpS group than in the S group. Mean total antioxidant capacity were lower in the S group than in the C group, and Spirulina reversed this change. Although not significantly different, IL-2 was lower in the S group than in the C group. However, in the SpS group, IL-2 increased due to Spirulina platensis mitigating effects of stress. Conclusion: Male rats fed a diet with Spirulina platensis could experience significantly milder physiological changes during stress, although stress patterns may be different. Exogenous antioxidant supplements merit further investigation in animals and humans where the endogenous defence mechanism against stress may not be sufficient.CPublication Thermoregulatory, cardiovascular, and metabolic responses to mild caloric restriction in the Brown Norway rat(Wiley, 2013-07-01) Aydın, Cenk; Gordon, Christopher J.; AYDIN, CENK; Veteriner Fakültesi; Fizyoloji Ana Bilim Dalı; 0000-0002-3090-0099; DWC-5118-2022Caloric restriction (CR) has been demonstrated to prolong the life span of a variety of species. CR-induced reduction in core temperature (Tc) is considered a key mechanism responsible for prolonging life span in rodents; however, little is known about the regulation of CR-induced hypothermia as a function of the circadian cycle. We assessed how mild CR that resulted in a 10% reduction in body weight affected the 24 h patterns of Tc as well as heart rate (HR) and motor activity (MA) of the Brown Norway rat. Telemetered rats were allowed to feed for 20 weeks ad libitum (AL) or given a CR diet. Tc, HR, and MA of CR rats exhibited nocturnal reductions and diurnal elevations, opposite to that of AL rats. The effects of CR appeared to peak at similar to 4 weeks. Metabolic rate (MR) and respiratory exchange ratio (RER) were measured overnight after 18 weeks of CR. MR and RER were elevated markedly at the time of feeding in CR rats and then declined during the night. We found that the pattern of Tc was altered with CR, characterized by elimination of high nocturnal Tc's typically observed in AL animals. In terms of mechanisms to prolong life span in CR animals, we suggest that the shift in the pattern of Tc during CR (i. e., elimination of high Tc's) may be as critical as the overall mean reduction in Tc. Future studies should address how the time of feeding may affect the thermoregulatory response in calorically restricted rats.Publication Caloric restriction in lean and obese strains of laboratory rat: Effects on body composition, metabolism, growth and overall health(Wiley, 2015-11-01) Aydın, Cenk; Jarema, K. A.; Phillips, P. M.; Gordon, C. J.; AYDIN, CENK; Veteriner Fakültesi; Fizyoloji Ana Bilim Dalı; CCF-5728-2022New FindingsWhat is the central question of this study? How do lean and obese rats respond physiologically to caloric restriction?What is the main finding and its importance? Obese rats show marked benefits compared with lean animals.Reduced body fat is associated with improved longevity with caloric restriction (CR) in rodents. Little is known regarding effects of CR in genetically lean versus obese strains. Long-Evans (LE) and Brown Norway (BN) rats make an ideal comparison for a CR study because the percentage body fat of young adult LE rats is double that of BN rats. Male LE and BN rats were either fed ad libitum (AL) or were calorically restricted to 80 or 90% of their AL weight. The percentages of fat, lean and fluid mass were measured non-invasively at 2- to 4-week intervals. Metabolic rate and respiratory quotient were measured after 3, 6, 9 and 12months of CR. Overall health was scored monthly. The percentage of fat of the LE strain decreased with CR, whereas the percentage of fat of the BN strain remained above the AL group for several months. The percentage of lean mass increased above the AL for both strains subjected to CR. The percentage of fluid was unaffected by CR. The average metabolic rate over 22h of the BN rats subjected to CR was reduced, whereas that of LE rats was increased slightly above the AL group. The respiratory quotient of BN rats was decreased with CR. Overall health of the CR LE group was significantly improved compared with that of the AL group, whereas health of the CR BN rats was impaired compared with the AL group. Overall, the lean BN and obese LE strains differ markedly in fat utilization and metabolic response to prolonged CR. There appears to be little benefit of CR in the lean strain.