Publication:
Olea europaea leaf extract suppress stemness-characteristics of gastric cancer via long non-coding rnas

dc.contributor.authorTekin, Çağla
dc.contributor.authorErçelik, Melis
dc.contributor.authorTezcan, Gülçin
dc.contributor.authorAksoy, Seçil Ak
dc.contributor.authorEgeli, Ünal
dc.contributor.authorÇeçener, Gülşah
dc.contributor.authorTunca, Berrin
dc.contributor.buuauthorTekin, Çağla
dc.contributor.buuauthorErçelik, Melis
dc.contributor.buuauthorTEZCAN, GÜLÇİN
dc.contributor.buuauthorAksoy, Seçil Ak
dc.contributor.buuauthorEGELİ, ÜNAL
dc.contributor.buuauthorÇEÇENER, GÜLŞAH
dc.contributor.buuauthorTUNCA, BERRİN
dc.contributor.departmentBursa Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyoloji Anabilim Dalı.
dc.contributor.departmentBursa Uludağ Üniversitesi/Diş Hekimliği Fakültesi/Temel Bilimler Bölümü.
dc.contributor.departmentBursa Uludağ Üniversitesi/İnegöl Meslek Yüksekokulu.
dc.contributor.orcid0000-0002-5956-8755
dc.contributor.orcid0000-0001-7904-883X
dc.contributor.orcid0000-0002-3820-424X
dc.contributor.orcid0000-0002-1619-6680
dc.contributor.researcheridAAH-3843-2020
dc.contributor.researcheridAAH-1420-2021
dc.contributor.researcheridADM-8457-2022
dc.contributor.researcheridAAP-9988-2020
dc.contributor.researcheridABI-6078-2020
dc.contributor.researcheridGDC-6329-2022
dc.contributor.researcheridEUG-3329-2022
dc.date.accessioned2024-09-24T05:51:04Z
dc.date.available2024-09-24T05:51:04Z
dc.date.issued2022-01-04
dc.description.abstractIntroduction: 5-Fluorouracil (5-FU) and Cisplatin (Cis) are insufficient to fight against stem-like cancer cell (CSC)-phenotype Gastric cancer (GC). Therefore, new therapeutic approaches are required to treat CSC-GC patients. Although the anticancer effect of Olea europaea leaf extract (OLE) has been described for multiple types of cancer, including GC, the potential of OLE to be used as a complementary to 5-FU and Cis to treat CSC-GC remains largely unknown. This study uniquely investigated the potential of OLE to be used as complementary to 5-FU-Cis therapy to alleviate the CSC-mediated aggressiveness of GC.Methods: The AGS cells, A GC cell line was treated with OLE-only and its combinations with 5-FU-Cis. Tumor aggressiveness, colony formation, and vascularization were analyzed using tumorsphere and clonogenic assays and ex-vivo analyses. In addition, their effect on the expression of CSC markers, including CD133, OCT4, NANOG, and SOX2 and LncRNA, PVT1, MALAT1, H19, and SNHG16, was investigated using RT-qPCR.Results: OLE-only and involvement of OLE to 5-FU+Cis treatment reduced the size of tumor-spheres (p < 0.0001), number of colonies (p < 0.0001), and vascularization. In addition, the OLE-5-FU-Cis combination decreased mRNA expression levels CSC markers and LncRNA, PVT1, H19, and SNHG16 expression levels compared to 5-FU+Cis (p < 0.05).Conclusion: OLE reduced the survival capacity of CSC phenotype cells by decreasing LncRNA PVT1, MALAT1, and H19 and facilitates the aggressive features of GC cells. Further analysis and validations are required; current findings suggest that OLE could be complementarily used to improve the effect of 5-FU+Cis therapy for GC.
dc.identifier.doi10.1016/j.eujim.2022.102099
dc.identifier.issn1876-3820
dc.identifier.urihttps://doi.org/10.1016/j.eujim.2022.102099
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S1876382022000014?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/11452/45086
dc.identifier.volume49
dc.identifier.wos000784299400018
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherElsevier Science Inc
dc.relation.bapTGA-2020-174
dc.relation.bapHDP (IIF)-2020/18
dc.relation.bap100/2000
dc.relation.journalEuropean Journal of Integrative Medicine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCells
dc.subjectExpression
dc.subjectAdenocarcinoma
dc.subject5-fluorouracil
dc.subjectAngiogenesis
dc.subjectFluorouracil
dc.subjectMetastasis
dc.subjectMechanisms
dc.subjectResponses
dc.subjectExposure
dc.subjectAnticancer drug
dc.subjectStem-like cancer cell
dc.subjectGastric cancer
dc.subjectLong non-coding rnas
dc.subjectTumor sphere
dc.subjectIntegrative & complementary medicine
dc.titleOlea europaea leaf extract suppress stemness-characteristics of gastric cancer via long non-coding rnas
dc.typeArticle
dspace.entity.typePublication
relation.isAuthorOfPublicatione171a866-0a2e-4df4-9f4b-d9058971c979
relation.isAuthorOfPublication051cf631-d214-4c8f-b1f5-fa1d27d5269c
relation.isAuthorOfPublicationae26ce61-4a33-4336-9fe3-b40d1138c397
relation.isAuthorOfPublication121a3732-be5d-4aff-9195-357c8347daca
relation.isAuthorOfPublication.latestForDiscoverye171a866-0a2e-4df4-9f4b-d9058971c979

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