Publication:
Choline increases acetylcholine release and protects against the stimulation-induced decrease in phosphatide levels within membranes of rat corpus striatum

dc.contributor.authorWurtman, Richard J.
dc.contributor.authorMauron, Charlotte
dc.contributor.authorBlusztajn, Jan Krzysztof
dc.contributor.buuauthorUlus, İsmail Hakkı
dc.contributor.departmentTıp Fakültesi
dc.contributor.departmentFarmakoloji Ana Bilim Dalı
dc.contributor.orcid0000-0001-8438-3122tr_TR
dc.contributor.researcheridD-5340-2015tr_TR
dc.date.accessioned2021-06-21T09:00:35Z
dc.date.available2021-06-21T09:00:35Z
dc.date.issued1988-04-10
dc.description.abstractThis study examined the possibility that membrane phospholipids might be a source of choline used for acetylcholine (ACh) synthesis. Slices of rat striatum or cerebellum were superfused with a choline-free or choline-containing (10, 20 or 40 μM) physiological solution with eserine, for alternating 20 min periods of rest or electrical stimulation. Superfusion media were assayed for choline and ACh, and slice samples taken before and after stimulation were assayed for choline, ACh, various phospholipids, protein and DNA. The striatal slices were able to sustain the stimulation-induced release of ACh, releasing a total of about 3 times their initial ACh contents during the 8 periods of stimulation and rest. During these 8 cycles, 885 pmol/μg DNA free choline was released from the slices into the medium, an amount about 45-fold higher than the initial or final free choline levels in the slices. Although repeated stimulation of the striatal slices failed to affect tissue levels of free choline or ACh, this treatment did cause significant, dose-related (i.e., number of stimulation periods) stoichiometric decreases in tissue levels of phosphatidylcholine (PC) and of the other major phospholipids; tissue protein levels also declined significantly. Addition of exogenous choline to the superfusion medium produced dose-related increases in resting and evoked ACh release. The choline also fully protected the striatal slices from phospholipid depletion for as many as 6 stimulation periods. Cerebellar slices liberated large amounts of free choline into the medium but did not release measurable quantities of ACh; their phospholipid and protein levels did not decline with electrical stimulation. These data show that membrane phospholipids constitute a reservoir of free choline that can be used for ACh synthesis. When free choline is in short supply, ACh synthesis and release are sustained at the expense of this reservoir. The consequent reduction in membrane PC apparently is associated with a depletion of cellular membrane. The use of free choline by cholinergic neurons for two purposes, the syntheses of both ACh and membrane phospholipids, may thus impart vulnerability to them in situations where the supply of free choline is less than that needed for acetylation.en_US
dc.identifier.citationUlus, İ. H.vd. (1989). "Choline increases acetylcholine release and protects against the stimulation-induced decrease in phosphatide levels within membranes of rat corpus striatum". Brain Research, 484(1-2), 217-227.en_US
dc.identifier.endpage227tr_TR
dc.identifier.issn0006-8993
dc.identifier.issue1-2tr_TR
dc.identifier.pubmed2713682tr_TR
dc.identifier.scopus2-s2.0-0024598955tr_TR
dc.identifier.startpage217tr_TR
dc.identifier.urihttps://doi.org/10.1016/0006-8993(89)90364-8
dc.identifier.urihttps://www.sciencedirect.com/science/article/abs/pii/0006899389903648
dc.identifier.urihttp://hdl.handle.net/11452/20672
dc.identifier.volume484tr_TR
dc.identifier.wosA1989U226100022
dc.indexed.pubmedPubmeden_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.collaborationYurt dışıtr_TR
dc.relation.journalBrain Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCholineen_US
dc.subjectAcetylcholineen_US
dc.subjectPhospholipiden_US
dc.subjectPhosphatidylcholinen_US
dc.subjectPrecursoren_US
dc.subjectStriatumen_US
dc.subject.emtreeCholineen_US
dc.subject.emtreeDNAen_US
dc.subject.emtreePhosphatidylcholineen_US
dc.subject.emtreePhospholipiden_US
dc.subject.emtreeRadioisotopeen_US
dc.subject.emtreeAcetylcholine releaseen_US
dc.subject.emtreeAnimal cellen_US
dc.subject.emtreeCorpus striatumen_US
dc.subject.emtreeMaleen_US
dc.subject.emtreeNonhumanen_US
dc.subject.emtreeRaten_US
dc.subject.meshAcetylcholineen_US
dc.subject.meshAnimalen_US
dc.subject.meshCerebellumen_US
dc.subject.meshCholineen_US
dc.subject.meshCorpus Striatumen_US
dc.subject.meshElectric stimulationen_US
dc.subject.meshIn vitroen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane lipidsen_US
dc.subject.meshPhospholipidsen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, inbred strainsen_US
dc.subject.wosNeurosciencesen_US
dc.titleCholine increases acetylcholine release and protects against the stimulation-induced decrease in phosphatide levels within membranes of rat corpus striatumen_US
dc.typeArticle
dspace.entity.typePublication
local.contributor.departmentTıp Fakültesi/Farmakoloji Ana Bilim Dalıtr_TR
local.indexed.atScopus

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