Publication:
Evaluation of 2015-2016 MOTAKK HBV DNA and HCV RNA external quality assessment national program results

dc.contributor.authorKaratayli, Ersin
dc.contributor.authorSoydemir, Ege
dc.contributor.authorAksoy, Zeynep Busra
dc.contributor.authorKizilpinar, Mehtap
dc.contributor.authorAltay Kocak, Aylin
dc.contributor.authorKaratayli, Senem Ceren
dc.contributor.authorYurdcu, Esra
dc.contributor.authorYildirim, Umut
dc.contributor.authorGuriz, Haluk
dc.contributor.authorBozdayi, Gulendam
dc.contributor.authorYurdaydin, Cihan
dc.contributor.authorIlhan, Osman
dc.contributor.authorYildirim, Yasin
dc.contributor.authorBozdayi, A. Mithat
dc.contributor.authorOguz, Acelya Yalcintas
dc.contributor.authorBaris, Ahmet
dc.contributor.authorAlp, Alpaslan
dc.contributor.authorAksozek, Alper
dc.contributor.authorSayiner, Arzu
dc.contributor.authorKaragul, Aydan
dc.contributor.authorOrdu, Aylin
dc.contributor.authorIstanbullu, Aye
dc.contributor.authorOtlu, Baris
dc.contributor.authorAridogan, Buket
dc.contributor.authorAksu, Burak
dc.contributor.authorBuruk, C. Kurtulus
dc.contributor.authorKarahan, Ceren
dc.contributor.authorGuney, Cakir
dc.contributor.authorToksoz, Devrim
dc.contributor.authorYildirim, Dilara
dc.contributor.authorColak, Dilek
dc.contributor.authorDaglar, Duygu Eren
dc.contributor.authorFindik, Duygu
dc.contributor.authorKas, Elif
dc.contributor.authorCaliskan, Emel
dc.contributor.authorZeyrek, Fadile Yildiz
dc.contributor.authorArslan, Fatma
dc.contributor.authorDemir, Feyza
dc.contributor.authorMilletli, Fikriye
dc.contributor.authorKibar, Filiz
dc.contributor.authorOzdincer, Furkan
dc.contributor.authorDundar, Gulnur
dc.contributor.authorArslan, Hande
dc.contributor.authorAgca, Harun
dc.contributor.authorAliskan, Hikmet Eda
dc.contributor.authorGuducuoglu, Huseyin
dc.contributor.authorFidan, Isil
dc.contributor.authorAkyar, Isin
dc.contributor.authorAfsar, Ilhan
dc.contributor.authorKaleli, Ilknur
dc.contributor.authorDonmez, Ismail
dc.contributor.authorYanik, Kemalettin
dc.contributor.authorMidilli, Kenan
dc.contributor.authorCubukcu, Kivanc
dc.contributor.authorOzdemir, Mehmet
dc.contributor.authorAcar, Melek
dc.contributor.authorYalinay, Meltem
dc.contributor.authorKuskucu, Mert Ahmet
dc.contributor.authorBakici, Mustafa Zahir
dc.contributor.authorAydin, Neriman
dc.contributor.authorYilmaz, Neziha
dc.contributor.authorCeken, Nihan
dc.contributor.authorZiyade, Nihan
dc.contributor.authorYilmaz, Nisel
dc.contributor.authorOzgumus, Osman Birol
dc.contributor.authorGitmisoglu, Ozlem
dc.contributor.authorDemirgan, Recep
dc.contributor.authorKesli, Recep
dc.contributor.authorGuckan, Ridvan
dc.contributor.authorSertoz, Ruchan
dc.contributor.authorAkgun, Sadik
dc.contributor.authorAksaray, Sebahat
dc.contributor.authorTezcan, Seda
dc.contributor.authorKaygusuz, Sedat
dc.contributor.authorGokahmetoglu, Selma
dc.contributor.authorMese, Sevim
dc.contributor.authorBayik, Seyit Ahmet
dc.contributor.authorAkcali, Sinem
dc.contributor.authorGurcan, Saban
dc.contributor.authorKarsligil, Tekin
dc.contributor.authorUs, Tercan
dc.contributor.authorOzekinci, Tuncer
dc.contributor.authorPilgir, Tulin
dc.contributor.authorAslan, Ugur
dc.contributor.authorDinc, Ugur
dc.contributor.authorCoskun, Umut Safiye Say
dc.contributor.authorCetinkol, Yeliz
dc.contributor.authorKeskin, Yusuf
dc.contributor.authorAyaydin, Zeynep
dc.contributor.authorToraman, Zulal Asci
dc.contributor.buuauthorAĞCA, HARUN
dc.contributor.departmentUludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji Anabilim Dalı.
dc.contributor.orcid0000-0002-2651-2034
dc.contributor.researcheridISU-9626-2023
dc.date.accessioned2024-10-03T11:25:53Z
dc.date.available2024-10-03T11:25:53Z
dc.date.issued2018-10-01
dc.description.abstractMOTAKK, as a national external quality control program has been launched to evaluate the molecular detection of viral infections including HBV DNA and HCV RNA in molecular microbiology diagnostic laboratories in Turkey. This program is prepared in compliance with ISO 17043:2010 (Conformity assessment general requirements for proficiency testing) standards, and aims to take the place of external quality control programs from abroad, contributing to standardization and accuracy of molecular diagnostic tests in our country. The aim of this study was to evaluate 2015 and 2016 results of the MOTAKK External Quality Control Program for HBV DNA and HCV RNA viral load. The calls were announced on the web page of MOTAKK (www.motakk.org). The quality control samples were sent to participating laboratories in 2015 and 2016. Main stocks were prepared from patients with chronic hepatitis B and C who had viral load detection with reference methods according to WHO reference materials for viral load studies to improve quality control sera. From these main stocks, samples with different viral loads were prepared from dilutions of plasma with HBV, HCV, HAV, HIV, Parvovirus B19 and CMV negative serologic markers. Quality control samples were sent to the participating laboratories along with the negative samples in the cold chain. The laboratories accomplished the related tests within 2-3 weeks and entered their results on the MOTAKK web page. These results were analysed according to ISO 13528 (Statistical methods for use in proficiency testing by interlaboratory comparison) and scoring reports were created by a software developed by MOTAKK and sent to participating labs. Each laboratory evaluated their own results in comparison with the other laboratory results, reassessed the tests via observing the distance from the mean result and the reference values. The number of laboratories participating in the HBV DNA and HCV RNA external quality control program was 70-73 in 2015-2016. Participants were able to comply with the program tools, registering, entering results and receiving the results reports problem. In HBV panel, 72.6-89.1% and 84.7-90.3% of the participant laboratories were in 1 standard deviation (SD) in 2015-2016, respectively. In HCV panel, 70.8-89.1% and 84.7-90.3% of the participant laboratories were in 1 SD in 2015-2016, respectively. A national external quality control program for HBV DNA and HCV RNA in Turkey has been prepared for the first time with this project and implemented successfully. All the data provided in the MOTAKK external quality control program final report, compensate all the data provided by the quality control program final reports from abroad; additionally, the report allows comparison of used technologies and commercial products.
dc.identifier.doi10.5578/mb.67081
dc.identifier.endpage366
dc.identifier.issn0374-9096
dc.identifier.issue4
dc.identifier.startpage348
dc.identifier.urihttps://doi.org/10.5578/mb.67081
dc.identifier.urihttp://www.mikrobiyolbul.org/linkout.aspx?pmid=30522421
dc.identifier.urihttps://hdl.handle.net/11452/45795
dc.identifier.volume52
dc.identifier.wos000454987700003
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherAnkara Microbiology Soc
dc.relation.journalMikrobiyoloji Bülteni
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.relation.tubitak213S130 - 1001
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectReal-time pcr
dc.subjectHepatitis-b
dc.subjectClinical microbiology
dc.subjectProficiency
dc.subjectTests
dc.subjectHbv dna
dc.subjectHcv rna
dc.subjectExternal quality control
dc.subjectViral load
dc.subjectMicrobiology
dc.titleEvaluation of 2015-2016 MOTAKK HBV DNA and HCV RNA external quality assessment national program results
dc.typeArticle
dspace.entity.typePublication
relation.isAuthorOfPublicationeeb102e3-a297-417f-962b-8b6991f5b89b
relation.isAuthorOfPublication.latestForDiscoveryeeb102e3-a297-417f-962b-8b6991f5b89b

Files

Collections