Publication: Olea europaea L. leaf extract Attenuates Temozolomide-induced senescence-associated secretion phenotype in glioblastoma
dc.contributor.author | Erçelik, Melis | |
dc.contributor.author | Tunca, Berrin | |
dc.contributor.author | Ak Aksoy, Seçil | |
dc.contributor.author | Tekin, Çağla | |
dc.contributor.author | Tezcan, Gülçin | |
dc.contributor.buuauthor | Erçelik, Melis | |
dc.contributor.buuauthor | TUNCA, BERRİN | |
dc.contributor.buuauthor | Ak Aksoy, Seçil | |
dc.contributor.buuauthor | Tekin, Çağla | |
dc.contributor.buuauthor | TEZCAN, GÜLÇİN | |
dc.contributor.department | Bursa Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyoloji Anabilim Dalı. | |
dc.contributor.department | Bursa Uludağ Üniversitesi/İnegöl Meslek Yüksekokulu. | |
dc.contributor.department | Bursa Uludağ Üniversitesi/Diş Hekimliği Fakültesi/Temel Bilimler Bölümü. | |
dc.contributor.orcid | 0000-0002-1619-6680 | |
dc.contributor.orcid | 0000-0002-5956-8755 | |
dc.contributor.researcherid | AAH-3843-2020 | |
dc.contributor.researcherid | ABI-6078-2020 | |
dc.contributor.researcherid | JKU-0358-2023 | |
dc.contributor.researcherid | IVC-4516-2023 | |
dc.contributor.researcherid | JKO-1775-2023 | |
dc.date.accessioned | 2024-09-26T08:36:13Z | |
dc.date.available | 2024-09-26T08:36:13Z | |
dc.date.issued | 2023-04-01 | |
dc.description.abstract | Objectives: The purpose of this study was to investigate the effect of Olea europaea L. leaf extract (OLE) on senescence and senescence-associated secretory phenotype (SASP) caused by temozolomide (TMZ) in glioblastoma (GB).Materials and Methods: A senescence beta-galactosidase assay and a colony formation assay were used to determine the effects of OLE, TMZ, and OLE + TMZ on the cellular senescence and aggressiveness of GB cell lines T98G and U87MG. mRNA expression levels of p53, a senescence factor, interleukin (IL)-6, matrix metalloproteinases (MMP)-9, and nuclear factor kappa B1 (NF-kappa B1) as SASP factors and Bcl-2 and Bax as senolytic markers were assessed using quantitative reverse transcription-real-time polymerase chain reaction. Cells were double-stained with acridine orange and propidium iodide to observe the cell morphology.Results: TMZ increased the senescence rate of GB cells (p<0.001). Besides, OLE + TMZ reduced the proportion of senescent cells (p<0.001) and their capability to form colonies compared to TMZ-only-treated cells. Additionally, OLE + TMZ co-treatment elevated the mRNA expression levels of MMP-9, IL-6, NF-kappa B1, p53, and the Bax/Bcl-2 ratio compared to TMZ-only treatment. Especially in U87MG cells, involvement of OLE in TMZ treatments increased more than six times in the Bax/Bcl-2 ratio compared to TMZ-only, which induced the apoptosis-like morphological features (p<0.0001).Conclusion: Collectively, our findings presented the inhibitory effect of OLE on TMZ-mediated SASP-factor production in GB and, accordingly, its potential contribution to elongate the time of recurrence. | |
dc.description.sponsorship | Yükseköğretim Kurulu - 100/2000 | |
dc.identifier.doi | 10.4274/tjps.galenos.2022.57639 | |
dc.identifier.endpage | 77 | |
dc.identifier.issn | 1304-530X | |
dc.identifier.issue | 2 | |
dc.identifier.startpage | 68 | |
dc.identifier.uri | https://doi.org/10.4274/tjps.galenos.2022.57639 | |
dc.identifier.uri | https://turkjps.org/articles/doi/tjps.galenos.2022.57639 | |
dc.identifier.uri | https://hdl.handle.net/11452/45296 | |
dc.identifier.volume | 20 | |
dc.identifier.wos | 000995975600001 | |
dc.indexed.wos | WOS.ESCI | |
dc.language.iso | en | |
dc.publisher | Galenos Yayınevi | |
dc.relation.journal | Turkish Journal of Pharmaceutical Sciences | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | |
dc.relation.tubitak | 118S799 | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | Cell senescence | |
dc.subject | In-vitro | |
dc.subject | Apoptosis | |
dc.subject | Expression | |
dc.subject | Differentiation | |
dc.subject | Methylation | |
dc.subject | Improves | |
dc.subject | P53 | |
dc.subject | Glioblastoma | |
dc.subject | Olea europaea leaf extract | |
dc.subject | Temozolomide | |
dc.subject | Senescence | |
dc.subject | Sasp | |
dc.subject | Pharmacology & pharmacy | |
dc.title | Olea europaea L. leaf extract Attenuates Temozolomide-induced senescence-associated secretion phenotype in glioblastoma | |
dc.type | Article | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | 121a3732-be5d-4aff-9195-357c8347daca | |
relation.isAuthorOfPublication | e171a866-0a2e-4df4-9f4b-d9058971c979 | |
relation.isAuthorOfPublication.latestForDiscovery | 121a3732-be5d-4aff-9195-357c8347daca |
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