Selection of salt tolerant lines at cell level using gamma ray with callus and suspension culture techniques in black carrots (Daucus carota L. ssp. sativus var. atrorubens Alef.)

Abstract

The main objective was to select salt tolerant lines at the cell level of Hatay region's black carrot (Daucus carota L. ssp. sativus var. atrorubens Alef.) using callus and suspension culture techniques combined with gamma rays. Hypocotyl explants of in vitro grown plantlets was used for callus induction. Effective mutation dose was determined by gamma radiation treatment at various doses (0, 5, 10, 20, 30, 40, 50, 60 Gy) to black carrot calli after in vitro optimization steps. According to regression analysis, the number of plants regenerated from calli was found 8.36 Gy as effective dose. In the ongoing study, calli with 7 Gy, 8 Gy and 9 Gy gamma rays were multiplied by subculture for 5 times. Shoot induction was achieved in medium containing 1 mg L-1 BAP concentration. Average plant height, root length and branching number parameters of plants regenerated from calli were determined. Salt stress was applied to the plants acclimatized from in vitro to the climate chambers. changes in the amount of peroxidase (POD) and superoxide dismutase (SOD) activities of antioxidant enzymes and the changes in lipid peroxidation were revealed in leaf samples taken from plants that continued to live in a salty environment after the 14 days of the treatment. At the end of the study, salt tolerance increased in mutant plants have the plant number of 8-21, 9-19, 7-9, 9-2 and 9-8 compared to the control, and these were determined as possible mutant plants.